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Effect of phenotypic switching on biofilm traits in Candida tropicalis.
Microb Pathog. 2020 Jun 17; 149:104346.MP

Abstract

Candida tropicalis can undergo multiple forms of phenotypic switching. We have reported a switching system in C. tropicalis that is associated with changes in virulence attributes. We aimed to assess biofilm formation by distinct switch states of C. tropicalis and evaluate whether their sessile cells exhibit altered virulence traits. C. tropicalis strains included the parental phenotype (a clinical isolate) and four switch phenotypes (crepe, rough, revertant of crepe and revertant of rough). Biofilm formation and adhesion capability of sessile cells on polystyrene were assessed through quantification of total biomass. Filamentous forms were characterized by direct counting of sessile cells. A virulence assay was conducted using the Galleria mellonella infection model. Switch variants (crepe and rough) and their revertant counterparts produced higher biofilm biomass (P < 0.05) than the parental strain. Additionally, filamentous forms were enriched among sessile cells of switched strains compared to those observed for sessile cells of the parental strain, with the exception of the revertant of rough. Sessile cells of switched strains showed higher adhesion to polystyrene compared to the parental strain. Sessile cells of the crepe variant and its revertant strain (RC) exhibited higher virulence against G. mellonella larvae than sessile cells of the parental strain. Our findings indicate that switching events in C. tropicalis affect biofilm development and that sessile cells of distinct switch states may exhibit increased adhesion ability and enhanced virulence towards G. mellonella larvae.

Authors+Show Affiliations

Department of Microbiology, Paraná State University of Londrina, Paraná, Brazil.Department of Microbiology, Paraná State University of Londrina, Paraná, Brazil.Department of Microbiology, Paraná State University of Londrina, Paraná, Brazil.Department of Food Technology, Technological Federal University of Paraná, Londrina, Paraná, Brazil.Department of Microbiology, Paraná State University of Londrina, Paraná, Brazil. Electronic address: furlaneto@uel.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32562809

Citation

Moralez, AlaneT P., et al. "Effect of Phenotypic Switching On Biofilm Traits in Candida Tropicalis." Microbial Pathogenesis, vol. 149, 2020, p. 104346.
Moralez AP, Perini HF, Paulo EA, et al. Effect of phenotypic switching on biofilm traits in Candida tropicalis. Microb Pathog. 2020;149:104346.
Moralez, A. P., Perini, H. F., Paulo, E. A., Furlaneto-Maia, L., & Furlaneto, M. C. (2020). Effect of phenotypic switching on biofilm traits in Candida tropicalis. Microbial Pathogenesis, 149, 104346. https://doi.org/10.1016/j.micpath.2020.104346
Moralez AP, et al. Effect of Phenotypic Switching On Biofilm Traits in Candida Tropicalis. Microb Pathog. 2020 Jun 17;149:104346. PubMed PMID: 32562809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of phenotypic switching on biofilm traits in Candida tropicalis. AU - Moralez,AlaneT P, AU - Perini,Hugo F, AU - Paulo,Eloiza A, AU - Furlaneto-Maia,Luciana, AU - Furlaneto,Marcia C, Y1 - 2020/06/17/ PY - 2020/04/05/received PY - 2020/06/02/revised PY - 2020/06/11/accepted PY - 2020/6/21/pubmed PY - 2020/6/21/medline PY - 2020/6/21/entrez KW - Adhesion KW - Galleria mellonella KW - Sessile cells KW - Virulence KW - switch States SP - 104346 EP - 104346 JF - Microbial pathogenesis JO - Microb. Pathog. VL - 149 N2 - Candida tropicalis can undergo multiple forms of phenotypic switching. We have reported a switching system in C. tropicalis that is associated with changes in virulence attributes. We aimed to assess biofilm formation by distinct switch states of C. tropicalis and evaluate whether their sessile cells exhibit altered virulence traits. C. tropicalis strains included the parental phenotype (a clinical isolate) and four switch phenotypes (crepe, rough, revertant of crepe and revertant of rough). Biofilm formation and adhesion capability of sessile cells on polystyrene were assessed through quantification of total biomass. Filamentous forms were characterized by direct counting of sessile cells. A virulence assay was conducted using the Galleria mellonella infection model. Switch variants (crepe and rough) and their revertant counterparts produced higher biofilm biomass (P < 0.05) than the parental strain. Additionally, filamentous forms were enriched among sessile cells of switched strains compared to those observed for sessile cells of the parental strain, with the exception of the revertant of rough. Sessile cells of switched strains showed higher adhesion to polystyrene compared to the parental strain. Sessile cells of the crepe variant and its revertant strain (RC) exhibited higher virulence against G. mellonella larvae than sessile cells of the parental strain. Our findings indicate that switching events in C. tropicalis affect biofilm development and that sessile cells of distinct switch states may exhibit increased adhesion ability and enhanced virulence towards G. mellonella larvae. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/32562809/Effect_of_phenotypic_switching_on_biofilm_traits_in_Candida_tropicalis L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(20)30712-9 DB - PRIME DP - Unbound Medicine ER -
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