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Detection of benzodiazepines in decomposing rabbit tissues and certain necrophagic dipteran species of forensic importance.
Saudi J Biol Sci. 2020 Jul; 27(7):1691-1698.SJ

Abstract

The determination of benzodiazepines (carbamazepine and clobazam) in rabbit carcass tissues and larvae of three Calliphorid flies is described. After feeding the rabbits with lethal and toxic doses, samples of larvae and carcass tissues were studied. Residual drugs were determined using Ultra-high-performance liquid chromatography - quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS). Benzodiazepines and its main active metabolites have been detected in the rabbit tissues at different retention times depending on the dosage used (lethal or toxic). A total of 1150 insects were collected and 800 larvae of the flies Chrysomya albiceps, Lucilia sericata and L. silvarum were used in the analysis. The presence of benzodiazepines in the rabbit tissues has been shown to typically affect the larval development cycle of the three necrophagous flies. Chrysomya albiceps larvae feed on drugs developed faster, while the development of L. sericata and L. silvarum larvae slowed. These results indicate that drugs have an impact on the life cycles of insects, which suggests that the presence of these substances is a factor that needs to be taken into account when estimating the post-mortem interval (PMI).

Authors+Show Affiliations

Department of Natural Sciences and Life, Faculty of Science, University of August 20th 1955 Skikda, Algeria.Department of Natural Sciences and Life, Faculty of Science, University of August 20th 1955 Skikda, Algeria.Department of Natural Sciences and Life, Faculty of Science, University of August 20th 1955 Skikda, Algeria.Department of Zoology, College of Sciences, King Saud University, Riyadh 11451, Saudi Arabia.National Institute of Forensic Sciences and Criminology (INCC), Algiers, Algeria.National Institute of Forensic Sciences and Criminology (INCC), Algiers, Algeria.Department of Zoology, Faculty of Science, Minia University, El-Minia 61519, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32565684

Citation

Boulkenafet, Fouzi, et al. "Detection of Benzodiazepines in Decomposing Rabbit Tissues and Certain Necrophagic Dipteran Species of Forensic Importance." Saudi Journal of Biological Sciences, vol. 27, no. 7, 2020, pp. 1691-1698.
Boulkenafet F, Dob Y, Karroui R, et al. Detection of benzodiazepines in decomposing rabbit tissues and certain necrophagic dipteran species of forensic importance. Saudi J Biol Sci. 2020;27(7):1691-1698.
Boulkenafet, F., Dob, Y., Karroui, R., Al-Khalifa, M., Boumrah, Y., Toumi, M., & Mashaly, A. (2020). Detection of benzodiazepines in decomposing rabbit tissues and certain necrophagic dipteran species of forensic importance. Saudi Journal of Biological Sciences, 27(7), 1691-1698. https://doi.org/10.1016/j.sjbs.2020.04.044
Boulkenafet F, et al. Detection of Benzodiazepines in Decomposing Rabbit Tissues and Certain Necrophagic Dipteran Species of Forensic Importance. Saudi J Biol Sci. 2020;27(7):1691-1698. PubMed PMID: 32565684.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of benzodiazepines in decomposing rabbit tissues and certain necrophagic dipteran species of forensic importance. AU - Boulkenafet,Fouzi, AU - Dob,Yasmine, AU - Karroui,Roumaissa, AU - Al-Khalifa,Mohammed, AU - Boumrah,Yacine, AU - Toumi,Moussa, AU - Mashaly,Ashraf, Y1 - 2020/05/07/ PY - 2020/02/11/received PY - 2020/03/15/revised PY - 2020/04/27/accepted PY - 2020/6/23/entrez PY - 2020/6/23/pubmed PY - 2020/6/23/medline KW - Benzodiazepines KW - Calliphoridae KW - Development cycle KW - Drugs KW - Entomotoxicology SP - 1691 EP - 1698 JF - Saudi journal of biological sciences JO - Saudi J Biol Sci VL - 27 IS - 7 N2 - The determination of benzodiazepines (carbamazepine and clobazam) in rabbit carcass tissues and larvae of three Calliphorid flies is described. After feeding the rabbits with lethal and toxic doses, samples of larvae and carcass tissues were studied. Residual drugs were determined using Ultra-high-performance liquid chromatography - quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS). Benzodiazepines and its main active metabolites have been detected in the rabbit tissues at different retention times depending on the dosage used (lethal or toxic). A total of 1150 insects were collected and 800 larvae of the flies Chrysomya albiceps, Lucilia sericata and L. silvarum were used in the analysis. The presence of benzodiazepines in the rabbit tissues has been shown to typically affect the larval development cycle of the three necrophagous flies. Chrysomya albiceps larvae feed on drugs developed faster, while the development of L. sericata and L. silvarum larvae slowed. These results indicate that drugs have an impact on the life cycles of insects, which suggests that the presence of these substances is a factor that needs to be taken into account when estimating the post-mortem interval (PMI). SN - 1319-562X UR - https://www.unboundmedicine.com/medline/citation/32565684/Detection_of_benzodiazepines_in_decomposing_rabbit_tissues_and_certain_necrophagic_dipteran_species_of_forensic_importance L2 - https://linkinghub.elsevier.com/retrieve/pii/S1319-562X(20)30166-2 DB - PRIME DP - Unbound Medicine ER -
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