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[Advances in gene editing of Corynebacterium glutamate].
Sheng Wu Gong Cheng Xue Bao. 2020 May 25; 36(5):820-828.SW

Abstract

Corynebacterium glutamicum, an important microorganism to produce amino acids and organic acids, has been widely applied in food and medicine fields. Therefore, using editing tools to study the function of unknown genes in C. glutamicum has great significance for systematic development of industrial strain with efficient and novel production capability. Recently, gene editing has been greatly developed. Traditional gene editing based on homologous recombination and gene editing mediated by nuclease are successfully applied in C. glutamicum. Among these, the CRISPR system has been developed to be a main tool used for gene knockout of C. glutamicum due to its advantages of efficiency, simplicity and good target specificity. However, more efficient and reliable knockout system is still urgently demanded, to help develop high-performing strains in industrial application.

Authors+Show Affiliations

School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300451, China.School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300451, China.School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300451, China.School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300451, China.School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300451, China.School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300451, China.School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300451, China.School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300451, China.

Pub Type(s)

Journal Article
Review

Language

chi

PubMed ID

32567265

Citation

Yang, Juanjuan, et al. "[Advances in Gene Editing of Corynebacterium Glutamate]." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 36, no. 5, 2020, pp. 820-828.
Yang J, Ma X, Wang X, et al. [Advances in gene editing of Corynebacterium glutamate]. Sheng Wu Gong Cheng Xue Bao. 2020;36(5):820-828.
Yang, J., Ma, X., Wang, X., Zhang, Z., Wang, S., Qin, H., Mao, S., & Lu, F. (2020). [Advances in gene editing of Corynebacterium glutamate]. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 36(5), 820-828. https://doi.org/10.13345/j.cjb.190403
Yang J, et al. [Advances in Gene Editing of Corynebacterium Glutamate]. Sheng Wu Gong Cheng Xue Bao. 2020 May 25;36(5):820-828. PubMed PMID: 32567265.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Advances in gene editing of Corynebacterium glutamate]. AU - Yang,Juanjuan, AU - Ma,Xiaoyu, AU - Wang,Xiaorui, AU - Zhang,Zhaohui, AU - Wang,Shan, AU - Qin,Huimin, AU - Mao,Shuhong, AU - Lu,Fuping, PY - 2020/6/23/entrez PY - 2020/6/23/pubmed PY - 2020/6/25/medline KW - CRISPR KW - Corynebacterium glutamicum KW - gene editing KW - homologous recombination SP - 820 EP - 828 JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JO - Sheng Wu Gong Cheng Xue Bao VL - 36 IS - 5 N2 - Corynebacterium glutamicum, an important microorganism to produce amino acids and organic acids, has been widely applied in food and medicine fields. Therefore, using editing tools to study the function of unknown genes in C. glutamicum has great significance for systematic development of industrial strain with efficient and novel production capability. Recently, gene editing has been greatly developed. Traditional gene editing based on homologous recombination and gene editing mediated by nuclease are successfully applied in C. glutamicum. Among these, the CRISPR system has been developed to be a main tool used for gene knockout of C. glutamicum due to its advantages of efficiency, simplicity and good target specificity. However, more efficient and reliable knockout system is still urgently demanded, to help develop high-performing strains in industrial application. SN - 1872-2075 UR - https://www.unboundmedicine.com/medline/citation/32567265/[Advances_in_gene_editing_of_Corynebacterium_glutamate] L2 - https://doi.org/10.13345/j.cjb.190403 DB - PRIME DP - Unbound Medicine ER -