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Studies on DNA-related enzymes to elucidate molecular mechanisms underlying genetic information processing and their application in genetic engineering.
Biosci Biotechnol Biochem. 2020 Jun 21 [Online ahead of print]BB

Abstract

Recombinant DNA technology, in which artificially "cut and pasted" DNA in vitro is introduced into living cells, contributed extensively to the rapid development of molecular biology over the past 5 decades since the latter half of the 20th century. Although the original technology required special experiences and skills, the development of polymerase chain reaction (PCR) has greatly eased in vitro genetic manipulation for various experimental methods. The current development of a simple genome-editing technique using CRISPR-Cas9 gave great impetus to molecular biology. Genome editing is a major technique for elucidating the functions of many unknown genes. Genetic manipulation technologies rely on enzymes that act on DNA. It involves artificially synthesizing, cleaving, and ligating DNA strands by making good use of DNA-related enzymes present in organisms to maintain their life activities. In this review, I focus on key enzymes involved in the development of genetic manipulation technologies.

Authors+Show Affiliations

Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32567488

Citation

Ishino, Yoshizumi. "Studies On DNA-related Enzymes to Elucidate Molecular Mechanisms Underlying Genetic Information Processing and Their Application in Genetic Engineering." Bioscience, Biotechnology, and Biochemistry, 2020, pp. 1-18.
Ishino Y. Studies on DNA-related enzymes to elucidate molecular mechanisms underlying genetic information processing and their application in genetic engineering. Biosci Biotechnol Biochem. 2020.
Ishino, Y. (2020). Studies on DNA-related enzymes to elucidate molecular mechanisms underlying genetic information processing and their application in genetic engineering. Bioscience, Biotechnology, and Biochemistry, 1-18. https://doi.org/10.1080/09168451.2020.1778441
Ishino Y. Studies On DNA-related Enzymes to Elucidate Molecular Mechanisms Underlying Genetic Information Processing and Their Application in Genetic Engineering. Biosci Biotechnol Biochem. 2020 Jun 21;1-18. PubMed PMID: 32567488.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on DNA-related enzymes to elucidate molecular mechanisms underlying genetic information processing and their application in genetic engineering. A1 - Ishino,Yoshizumi, Y1 - 2020/06/21/ PY - 2020/6/23/entrez KW - DNA polymerase KW - genetic engineering KW - genome editing KW - nuclease KW - polymerase chain reaction SP - 1 EP - 18 JF - Bioscience, biotechnology, and biochemistry JO - Biosci. Biotechnol. Biochem. N2 - Recombinant DNA technology, in which artificially "cut and pasted" DNA in vitro is introduced into living cells, contributed extensively to the rapid development of molecular biology over the past 5 decades since the latter half of the 20th century. Although the original technology required special experiences and skills, the development of polymerase chain reaction (PCR) has greatly eased in vitro genetic manipulation for various experimental methods. The current development of a simple genome-editing technique using CRISPR-Cas9 gave great impetus to molecular biology. Genome editing is a major technique for elucidating the functions of many unknown genes. Genetic manipulation technologies rely on enzymes that act on DNA. It involves artificially synthesizing, cleaving, and ligating DNA strands by making good use of DNA-related enzymes present in organisms to maintain their life activities. In this review, I focus on key enzymes involved in the development of genetic manipulation technologies. SN - 1347-6947 UR - https://www.unboundmedicine.com/medline/citation/32567488/Studies_on_DNA-related_enzymes_to_elucidate_molecular_mechanisms_underlying_genetic_information_processing_and_their_application_in_genetic_engineering L2 - http://www.tandfonline.com/doi/full/10.1080/09168451.2020.1778441 DB - PRIME DP - Unbound Medicine ER -
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