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Impact of heat-inactivation on the detection of SARS-CoV-2 IgM and IgG antibody by ELISA.
Clin Chim Acta. 2020 Oct; 509:288-292.CC

Abstract

BACKGROUND

To establish a safe and accurate method for detecting SARS-CoV-2 IgM and IgG, we assessed the impact of sera after heat-inactivation on the SARS-CoV-2 IgM and IgG levels measured by ELISA-immunoassay.

METHODS

The serum samples of 62 patients with COVID-19 and 18 healthy controls were collected in Hankou's Hospital of Wuhan from February 27 to March 6, 2020. Before and after the samples were inactivated, the levels of IgM and IgG antibodies were measured.

RESULTS

The indexes of antibodies after inactivated were significantly higher than those in fresh sera, while the positive rates in all participants or in patients with COVID-19 did not change. The positive coincidence rate, negative coincidence rate and total coincidence rate of IgM antibodies before and after inactivation were 100.00% (55/55), 96.00% (24/25) and 98.75% (79/80), respectively (κ = 0.971, P < 0.001), while those for IgG antibodies were 98.21% (55/56), 91.67% (22/24) and 98.75% (79/80) respectively (κ = 0.910, P < 0.001). These results showed a good consistency.

CONCLUSIONS

Heating-activation does not decrease the diagnostic efficacy of SARS-CoV-2 IgM or IgG antibodies. Sera inactivated by heating at 56 °C for 30 min should be recommended to minimize the risk of virus contamination of laboratory staff.

Authors+Show Affiliations

Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280 Guangdong, China.Department of Medicine Laboratory, Hankou Hospital of Wuhan, Wuhan 430010, China.Department of Medicine Laboratory, Hankou Hospital of Wuhan, Wuhan 430010, China. Electronic address: whshkyyjyk@outlook.com.Hui Qiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: pbh@smu.edu.cn.Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280 Guangdong, China. Electronic address: wangqian@smu.edu.cn.Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: nfyyzhenglei@smu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32569631

Citation

Hu, Xiumei, et al. "Impact of Heat-inactivation On the Detection of SARS-CoV-2 IgM and IgG Antibody By ELISA." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 509, 2020, pp. 288-292.
Hu X, Zhang R, An T, et al. Impact of heat-inactivation on the detection of SARS-CoV-2 IgM and IgG antibody by ELISA. Clin Chim Acta. 2020;509:288-292.
Hu, X., Zhang, R., An, T., Li, Q., Situ, B., Ou, Z., Wu, C., Yang, B., Tian, P., Hu, Y., Ping, B., Wang, Q., & Zheng, L. (2020). Impact of heat-inactivation on the detection of SARS-CoV-2 IgM and IgG antibody by ELISA. Clinica Chimica Acta; International Journal of Clinical Chemistry, 509, 288-292. https://doi.org/10.1016/j.cca.2020.06.032
Hu X, et al. Impact of Heat-inactivation On the Detection of SARS-CoV-2 IgM and IgG Antibody By ELISA. Clin Chim Acta. 2020;509:288-292. PubMed PMID: 32569631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of heat-inactivation on the detection of SARS-CoV-2 IgM and IgG antibody by ELISA. AU - Hu,Xiumei, AU - Zhang,Ruyi, AU - An,Taixue, AU - Li,Qiang, AU - Situ,Bo, AU - Ou,Zihao, AU - Wu,Changmeng, AU - Yang,Biao, AU - Tian,Peifu, AU - Hu,Yuhai, AU - Ping,Baohong, AU - Wang,Qian, AU - Zheng,Lei, Y1 - 2020/06/20/ PY - 2020/05/11/received PY - 2020/06/09/revised PY - 2020/06/18/accepted PY - 2020/6/23/pubmed PY - 2020/9/1/medline PY - 2020/6/23/entrez KW - COVID-19 KW - ELISA KW - Heat-inactivation KW - SARS-CoV-2 SP - 288 EP - 292 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 509 N2 - BACKGROUND: To establish a safe and accurate method for detecting SARS-CoV-2 IgM and IgG, we assessed the impact of sera after heat-inactivation on the SARS-CoV-2 IgM and IgG levels measured by ELISA-immunoassay. METHODS: The serum samples of 62 patients with COVID-19 and 18 healthy controls were collected in Hankou's Hospital of Wuhan from February 27 to March 6, 2020. Before and after the samples were inactivated, the levels of IgM and IgG antibodies were measured. RESULTS: The indexes of antibodies after inactivated were significantly higher than those in fresh sera, while the positive rates in all participants or in patients with COVID-19 did not change. The positive coincidence rate, negative coincidence rate and total coincidence rate of IgM antibodies before and after inactivation were 100.00% (55/55), 96.00% (24/25) and 98.75% (79/80), respectively (κ = 0.971, P < 0.001), while those for IgG antibodies were 98.21% (55/56), 91.67% (22/24) and 98.75% (79/80) respectively (κ = 0.910, P < 0.001). These results showed a good consistency. CONCLUSIONS: Heating-activation does not decrease the diagnostic efficacy of SARS-CoV-2 IgM or IgG antibodies. Sera inactivated by heating at 56 °C for 30 min should be recommended to minimize the risk of virus contamination of laboratory staff. SN - 1873-3492 UR - https://www.unboundmedicine.com/medline/citation/32569631/Impact_of_heat_inactivation_on_the_detection_of_SARS_CoV_2_IgM_and_IgG_antibody_by_ELISA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-8981(20)30294-1 DB - PRIME DP - Unbound Medicine ER -