Citation
Chi, Xiangyang, et al. "A Neutralizing Human Antibody Binds to the N-terminal Domain of the Spike Protein of SARS-CoV-2." Science (New York, N.Y.), vol. 369, no. 6504, 2020, pp. 650-655.
Chi X, Yan R, Zhang J, et al. A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2. Science. 2020;369(6504):650-655.
Chi, X., Yan, R., Zhang, J., Zhang, G., Zhang, Y., Hao, M., Zhang, Z., Fan, P., Dong, Y., Yang, Y., Chen, Z., Guo, Y., Zhang, J., Li, Y., Song, X., Chen, Y., Xia, L., Fu, L., Hou, L., ... Chen, W. (2020). A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2. Science (New York, N.Y.), 369(6504), 650-655. https://doi.org/10.1126/science.abc6952
Chi X, et al. A Neutralizing Human Antibody Binds to the N-terminal Domain of the Spike Protein of SARS-CoV-2. Science. 2020 08 7;369(6504):650-655. PubMed PMID: 32571838.
TY - JOUR
T1 - A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2.
AU - Chi,Xiangyang,
AU - Yan,Renhong,
AU - Zhang,Jun,
AU - Zhang,Guanying,
AU - Zhang,Yuanyuan,
AU - Hao,Meng,
AU - Zhang,Zhe,
AU - Fan,Pengfei,
AU - Dong,Yunzhu,
AU - Yang,Yilong,
AU - Chen,Zhengshan,
AU - Guo,Yingying,
AU - Zhang,Jinlong,
AU - Li,Yaning,
AU - Song,Xiaohong,
AU - Chen,Yi,
AU - Xia,Lu,
AU - Fu,Ling,
AU - Hou,Lihua,
AU - Xu,Junjie,
AU - Yu,Changming,
AU - Li,Jianmin,
AU - Zhou,Qiang,
AU - Chen,Wei,
Y1 - 2020/06/22/
PY - 2020/05/08/received
PY - 2020/06/17/accepted
PY - 2020/6/24/pubmed
PY - 2020/8/25/medline
PY - 2020/6/24/entrez
SP - 650
EP - 655
JF - Science (New York, N.Y.)
JO - Science
VL - 369
IS - 6504
N2 - Developing therapeutics against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could be guided by the distribution of epitopes, not only on the receptor binding domain (RBD) of the Spike (S) protein but also across the full Spike (S) protein. We isolated and characterized monoclonal antibodies (mAbs) from 10 convalescent COVID-19 patients. Three mAbs showed neutralizing activities against authentic SARS-CoV-2. One mAb, named 4A8, exhibits high neutralization potency against both authentic and pseudotyped SARS-CoV-2 but does not bind the RBD. We defined the epitope of 4A8 as the N-terminal domain (NTD) of the S protein by determining with cryo-eletron microscopy its structure in complex with the S protein to an overall resolution of 3.1 angstroms and local resolution of 3.3 angstroms for the 4A8-NTD interface. This points to the NTD as a promising target for therapeutic mAbs against COVID-19.
SN - 1095-9203
UR - https://www.unboundmedicine.com/medline/citation/32571838/A_neutralizing_human_antibody_binds_to_the_N_terminal_domain_of_the_Spike_protein_of_SARS_CoV_2_
DB - PRIME
DP - Unbound Medicine
ER -