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Application of platelet-rich plasma in the in vitro production of bovine embryos.
Trop Anim Health Prod. 2020 Jun 23 [Online ahead of print]TA

Abstract

The aim of this study was to replace fetal bovine serum (FBS) with platelet-rich plasma (PRP) for in vitro production of bovine embryos. The maturation media (TCM-199 medium) for the cumulus-oocyte complexes (COCs) was supplemented with 5% (G5) and 10% (G10) PRP or 10% FBS (GC). After fertilization, the presumed zygotes were randomly distributed in culture medium supplemented with 5% (G5) and 10% (G10) PRP or 10% FBS (GC) for 7 days. Cumulus cell (CC) expansion was greater (P < 0.05) in the GC (88.9%) group than in G5 (34.1%) or G10 (50.0%). Nevertheless, the expansion of CCs in group G10 was greater than in G5 (P < 0.05). Cleavage was higher in group G5 (86.0%) than in G10 (79.0%) (P < 0.05) and did not differ from group GC (82.0%). The percentage of blastocysts in group G5 (50.0%) was higher than in CG (40.2%) and G10 (34.2%) (P < 0.05). In addition, the number of blastomeres was higher in G5 (159.0 ± 4.18) than in GC (132.4 ± 4.11) and in G10 (127.1 ± 5.88) (P < 0.05). The addition of PRP into the oocytes maturation medium is not beneficial. On the other hand, the PRP addition into the embryo culture medium at 5% concentration is recommended where it increased the quantity and quality of in vitro-produced bovine embryos.

Authors+Show Affiliations

Laboratório de Biotécnicas Reprodutivas, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil. pamramosd@gmail.com.Laboratório de Biotécnicas Reprodutivas, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.Laboratório de Biotécnicas Reprodutivas, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.Laboratório de Biotécnicas Reprodutivas, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.Laboratório de Biotécnicas Reprodutivas, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.Laboratório de Biotécnicas Reprodutivas, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.Apoyar Biotecnologia da Reprodução Animal, Juiz de Fora, MG, Brazil.Instituto Agronômico de Pernambuco, Arcoverde, PE, Brazil.Laboratório de Biotécnicas Reprodutivas, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.Laboratório de Biotécnicas Reprodutivas, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32572854

Citation

Ramos-Deus, Pamela, et al. "Application of Platelet-rich Plasma in the in Vitro Production of Bovine Embryos." Tropical Animal Health and Production, 2020.
Ramos-Deus P, Santos Nascimento P, Vieira JIT, et al. Application of platelet-rich plasma in the in vitro production of bovine embryos. Trop Anim Health Prod. 2020.
Ramos-Deus, P., Santos Nascimento, P., Vieira, J. I. T., Chaves, M. S., Albuquerque, K. A., Ferreira-Silva, J. C., Grázia, J. G. V., Santos Filho, A. S., Batista, A. M., Teixeira, V. W., & Oliveira, M. A. L. (2020). Application of platelet-rich plasma in the in vitro production of bovine embryos. Tropical Animal Health and Production. https://doi.org/10.1007/s11250-020-02307-5
Ramos-Deus P, et al. Application of Platelet-rich Plasma in the in Vitro Production of Bovine Embryos. Trop Anim Health Prod. 2020 Jun 23; PubMed PMID: 32572854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of platelet-rich plasma in the in vitro production of bovine embryos. AU - Ramos-Deus,Pamela, AU - Santos Nascimento,Pábola, AU - Vieira,Joane Isis Travassos, AU - Chaves,Maiana Silva, AU - Albuquerque,Karoline Antunes, AU - Ferreira-Silva,José Carlos, AU - Grázia,João Grabriel Viana, AU - Santos Filho,Antonio Santana, AU - Batista,André Mariano, AU - Teixeira,Valéria Wanderley, AU - Oliveira,Marcos Antonio Lemos, Y1 - 2020/06/23/ PY - 2020/01/19/received PY - 2020/05/20/accepted PY - 2020/6/24/entrez KW - Blastocyst KW - Cleavage KW - Growth factor KW - Oocyte JF - Tropical animal health and production JO - Trop Anim Health Prod N2 - The aim of this study was to replace fetal bovine serum (FBS) with platelet-rich plasma (PRP) for in vitro production of bovine embryos. The maturation media (TCM-199 medium) for the cumulus-oocyte complexes (COCs) was supplemented with 5% (G5) and 10% (G10) PRP or 10% FBS (GC). After fertilization, the presumed zygotes were randomly distributed in culture medium supplemented with 5% (G5) and 10% (G10) PRP or 10% FBS (GC) for 7 days. Cumulus cell (CC) expansion was greater (P < 0.05) in the GC (88.9%) group than in G5 (34.1%) or G10 (50.0%). Nevertheless, the expansion of CCs in group G10 was greater than in G5 (P < 0.05). Cleavage was higher in group G5 (86.0%) than in G10 (79.0%) (P < 0.05) and did not differ from group GC (82.0%). The percentage of blastocysts in group G5 (50.0%) was higher than in CG (40.2%) and G10 (34.2%) (P < 0.05). In addition, the number of blastomeres was higher in G5 (159.0 ± 4.18) than in GC (132.4 ± 4.11) and in G10 (127.1 ± 5.88) (P < 0.05). The addition of PRP into the oocytes maturation medium is not beneficial. On the other hand, the PRP addition into the embryo culture medium at 5% concentration is recommended where it increased the quantity and quality of in vitro-produced bovine embryos. SN - 1573-7438 UR - https://www.unboundmedicine.com/medline/citation/32572854/Application_of_platelet-rich_plasma_in_the_in_vitro_production_of_bovine_embryos L2 - https://dx.doi.org/10.1007/s11250-020-02307-5 DB - PRIME DP - Unbound Medicine ER -
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