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A single dose of ChAdOx1 MERS provides protective immunity in rhesus macaques.
Sci Adv. 2020 06; 6(24):eaba8399.SA

Abstract

Developing a vaccine to protect against the lethal effects of the many strains of coronavirus is critical given the current global pandemic. For Middle East respiratory syndrome coronavirus (MERS-CoV), we show that rhesus macaques seroconverted rapidly after a single intramuscular vaccination with ChAdOx1 MERS. The vaccine protected against respiratory injury and pneumonia and reduced viral load in lung tissue by several orders of magnitude. MERS-CoV replication in type I and II pneumocytes of ChAdOx1 MERS-vaccinated animals was absent. A prime-boost regimen of ChAdOx1 MERS boosted antibody titers, and viral replication was completely absent from the respiratory tract tissue of these rhesus macaques. We also found that antibodies elicited by ChAdOx1 MERS in rhesus macaques neutralized six different MERS-CoV strains. Transgenic human dipeptidyl peptidase 4 mice vaccinated with ChAdOx1 MERS were completely protected against disease and lethality for all different MERS-CoV strains. The data support further clinical development of ChAdOx1 MERS.

Authors+Show Affiliations

Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.The Jenner Institute, University of Oxford, Oxford, UK.The Jenner Institute, University of Oxford, Oxford, UK. Transmission Biology Group, The Pirbright Institute, Pirbright, Woking, UK.The Jenner Institute, University of Oxford, Oxford, UK.The Jenner Institute, University of Oxford, Oxford, UK.Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32577525

Citation

van Doremalen, Neeltje, et al. "A Single Dose of ChAdOx1 MERS Provides Protective Immunity in Rhesus Macaques." Science Advances, vol. 6, no. 24, 2020, pp. eaba8399.
van Doremalen N, Haddock E, Feldmann F, et al. A single dose of ChAdOx1 MERS provides protective immunity in rhesus macaques. Sci Adv. 2020;6(24):eaba8399.
van Doremalen, N., Haddock, E., Feldmann, F., Meade-White, K., Bushmaker, T., Fischer, R. J., Okumura, A., Hanley, P. W., Saturday, G., Edwards, N. J., Clark, M. H. A., Lambe, T., Gilbert, S. C., & Munster, V. J. (2020). A single dose of ChAdOx1 MERS provides protective immunity in rhesus macaques. Science Advances, 6(24), eaba8399. https://doi.org/10.1126/sciadv.aba8399
van Doremalen N, et al. A Single Dose of ChAdOx1 MERS Provides Protective Immunity in Rhesus Macaques. Sci Adv. 2020;6(24):eaba8399. PubMed PMID: 32577525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A single dose of ChAdOx1 MERS provides protective immunity in rhesus macaques. AU - van Doremalen,Neeltje, AU - Haddock,Elaine, AU - Feldmann,Friederike, AU - Meade-White,Kimberly, AU - Bushmaker,Trenton, AU - Fischer,Robert J, AU - Okumura,Atsushi, AU - Hanley,Patrick W, AU - Saturday,Greg, AU - Edwards,Nick J, AU - Clark,Madeleine H A, AU - Lambe,Teresa, AU - Gilbert,Sarah C, AU - Munster,Vincent J, Y1 - 2020/06/10/ PY - 2020/01/09/received PY - 2020/04/16/accepted PY - 2020/6/25/entrez PY - 2020/6/25/pubmed PY - 2020/9/22/medline SP - eaba8399 EP - eaba8399 JF - Science advances JO - Sci Adv VL - 6 IS - 24 N2 - Developing a vaccine to protect against the lethal effects of the many strains of coronavirus is critical given the current global pandemic. For Middle East respiratory syndrome coronavirus (MERS-CoV), we show that rhesus macaques seroconverted rapidly after a single intramuscular vaccination with ChAdOx1 MERS. The vaccine protected against respiratory injury and pneumonia and reduced viral load in lung tissue by several orders of magnitude. MERS-CoV replication in type I and II pneumocytes of ChAdOx1 MERS-vaccinated animals was absent. A prime-boost regimen of ChAdOx1 MERS boosted antibody titers, and viral replication was completely absent from the respiratory tract tissue of these rhesus macaques. We also found that antibodies elicited by ChAdOx1 MERS in rhesus macaques neutralized six different MERS-CoV strains. Transgenic human dipeptidyl peptidase 4 mice vaccinated with ChAdOx1 MERS were completely protected against disease and lethality for all different MERS-CoV strains. The data support further clinical development of ChAdOx1 MERS. SN - 2375-2548 UR - https://www.unboundmedicine.com/medline/citation/32577525/A_single_dose_of_ChAdOx1_MERS_provides_protective_immunity_in_rhesus_macaques_ L2 - https://doi.org/10.1126/sciadv.aba8399 DB - PRIME DP - Unbound Medicine ER -