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Pluripotency-associated genes reposition during early embryonic developmental stages in pigs.
Anim Sci J. 2020 Jan; 91(1):e13408.AS

Abstract

We examined the allelic expression and positioning of two pluripotency-associated genes, OCT4 and SOX2, and two housekeeping genes, ACTB and TUBA, in 4- and 8-cell porcine embryos utilizing RNA and DNA fluorescence in situ hybridization (FISH) in single blastomeres. The proportion of blastomeres expressing SOX2 bi-allelically increased from 45% at the 4-cell stage to 60% at the 8-cell stage. Moreover, in 8-cell embryos, SOX2 was expressed bi-allelically in significantly more blastomeres than was the case for OCT4, and this was associated with a tendency for SOX2 alleles to move toward the nuclear interior during 4- to 8-cell transition. However, the radial location of OCT4 alleles did not change significantly during this transition. The locations of active and inactive alleles based on DNA and RNA FISH signals were also calculated. Inactive OCT4 alleles were located in very close proximity to the nuclear membrane, whereas active OCT4 alleles were more centrally disposed in the nucleus. Nevertheless, the nuclear location of active and inactive SOX2 alleles did not change in either 4- or 8-cell blastomeres. Our RNA and DNA FISH data provide novel information on the allelic expression patterns and positioning of pluripotency-associated genes, OCT4 and SOX2, during embryonic genome activation in pigs.

Authors+Show Affiliations

Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan. Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.Institute of Physics, Vietnam Academy of Science and Technology, Hanoi, Vietnam.Institute of Physics, Vietnam Academy of Science and Technology, Hanoi, Vietnam.Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan. The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32578338

Citation

Nguyen, Hiep Thi, et al. "Pluripotency-associated Genes Reposition During Early Embryonic Developmental Stages in Pigs." Animal Science Journal = Nihon Chikusan Gakkaiho, vol. 91, no. 1, 2020, pp. e13408.
Nguyen HT, Nghia NT, Lien NTH, et al. Pluripotency-associated genes reposition during early embryonic developmental stages in pigs. Anim Sci J. 2020;91(1):e13408.
Nguyen, H. T., Nghia, N. T., Lien, N. T. H., Dang-Nguyen, T. Q., Men, N. T., Viet Linh, N., Xuan Nguyen, B., Noguchi, J., Kaneko, H., & Kikuchi, K. (2020). Pluripotency-associated genes reposition during early embryonic developmental stages in pigs. Animal Science Journal = Nihon Chikusan Gakkaiho, 91(1), e13408. https://doi.org/10.1111/asj.13408
Nguyen HT, et al. Pluripotency-associated Genes Reposition During Early Embryonic Developmental Stages in Pigs. Anim Sci J. 2020;91(1):e13408. PubMed PMID: 32578338.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pluripotency-associated genes reposition during early embryonic developmental stages in pigs. AU - Nguyen,Hiep Thi, AU - Nghia,Nguyen Trong, AU - Lien,Nghiem Thi Ha, AU - Dang-Nguyen,Thanh Quang, AU - Men,Nguyen Thi, AU - Viet Linh,Nguyen, AU - Xuan Nguyen,Bui, AU - Noguchi,Junko, AU - Kaneko,Hiroyuki, AU - Kikuchi,Kazuhiro, PY - 2020/04/07/received PY - 2020/05/13/revised PY - 2020/05/22/accepted PY - 2020/6/25/entrez PY - 2020/6/25/pubmed PY - 2020/6/25/medline KW - allelic expression KW - gene positioning KW - pluripotency-associated genes KW - porcine embryos SP - e13408 EP - e13408 JF - Animal science journal = Nihon chikusan Gakkaiho JO - Anim. Sci. J. VL - 91 IS - 1 N2 - We examined the allelic expression and positioning of two pluripotency-associated genes, OCT4 and SOX2, and two housekeeping genes, ACTB and TUBA, in 4- and 8-cell porcine embryos utilizing RNA and DNA fluorescence in situ hybridization (FISH) in single blastomeres. The proportion of blastomeres expressing SOX2 bi-allelically increased from 45% at the 4-cell stage to 60% at the 8-cell stage. Moreover, in 8-cell embryos, SOX2 was expressed bi-allelically in significantly more blastomeres than was the case for OCT4, and this was associated with a tendency for SOX2 alleles to move toward the nuclear interior during 4- to 8-cell transition. However, the radial location of OCT4 alleles did not change significantly during this transition. The locations of active and inactive alleles based on DNA and RNA FISH signals were also calculated. Inactive OCT4 alleles were located in very close proximity to the nuclear membrane, whereas active OCT4 alleles were more centrally disposed in the nucleus. Nevertheless, the nuclear location of active and inactive SOX2 alleles did not change in either 4- or 8-cell blastomeres. Our RNA and DNA FISH data provide novel information on the allelic expression patterns and positioning of pluripotency-associated genes, OCT4 and SOX2, during embryonic genome activation in pigs. SN - 1740-0929 UR - https://www.unboundmedicine.com/medline/citation/32578338/Pluripotency-associated_genes_reposition_during_early_embryonic_developmental_stages_in_pigs L2 - https://doi.org/10.1111/asj.13408 DB - PRIME DP - Unbound Medicine ER -
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