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Stability of the Antimicrobial Capacity of Human Milk Against Cronobacter Sakazakii During Handling.
J Hum Lact. 2020 Jun 24 [Online ahead of print]JH

Abstract

BACKGROUND

Neonatal infections with Cronobacter sakazakii have recently been associated with the consumption of expressed human milk.

STUDY AIMS

(1) To evaluate whether human milk has antimicrobial capacity against C. sakazakii and (2) to determine the stability of its capacity when it is subjected to various treatments.

METHODS

The antimicrobial capacity of human milk against C. sakazakii was evaluated using an observational, cross-sectional, comparative design. Mature human milk samples (N = 29) were subjected to different treatments. After incubation at 37°C for 72 hr, samples were compared with fresh milk on the stability of their antimicrobial capacity. Two-way analysis of variance (ANOVA) was performed.

RESULTS

In fresh milk, counts of C. sakazakii were reduced by 47.26% (SD = 6.74) compared to controls. In treated milk, reductions were: refrigeration at 4°C for 72 hr (M = 33.84, SD = 13.84), freezing at -20°C for 1, 2, and 3 months (M = 40.31, SD = 9.10; M = 35.96, SD = 9.39; M = 26.20, SD = 13.55, respectively), Holder pasteurization (M = 23.56, SD = 15.61), and human milk bank treatment with (M = 14.37, SD = 18.02) and without bovine fortifier (M = 3.70, SD = 23.83). There were significant differences (p < .05) between fresh and treated milk.

CONCLUSIONS

Human milk has antimicrobial capacity against C. sakazakii. However, its capacity is negatively influenced by common preservation and hygienization methods. Milk should be stored refrigerated for a maximum of 72 hr or frozen for a short period of time.

Authors+Show Affiliations

16731 Department of Animal Production and Food Science and Technology, University CEU-Cardenal Herrera, CEU Universities, Valencia, Spain.16731 Department of Pharmacy, University CEU-Cardenal Herrera, CEU Universities, Valencia, Spain.16731 Department of Animal Production and Food Science and Technology, University CEU-Cardenal Herrera, CEU Universities, Valencia, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32579054

Citation

Fernández-Pastor, Sandra, et al. "Stability of the Antimicrobial Capacity of Human Milk Against Cronobacter Sakazakii During Handling." Journal of Human Lactation : Official Journal of International Lactation Consultant Association, 2020, p. 890334420932574.
Fernández-Pastor S, Castelló DS, López-Mendoza MC. Stability of the Antimicrobial Capacity of Human Milk Against Cronobacter Sakazakii During Handling. J Hum Lact. 2020.
Fernández-Pastor, S., Castelló, D. S., & López-Mendoza, M. C. (2020). Stability of the Antimicrobial Capacity of Human Milk Against Cronobacter Sakazakii During Handling. Journal of Human Lactation : Official Journal of International Lactation Consultant Association, 890334420932574. https://doi.org/10.1177/0890334420932574
Fernández-Pastor S, Castelló DS, López-Mendoza MC. Stability of the Antimicrobial Capacity of Human Milk Against Cronobacter Sakazakii During Handling. J Hum Lact. 2020 Jun 24;890334420932574. PubMed PMID: 32579054.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stability of the Antimicrobial Capacity of Human Milk Against Cronobacter Sakazakii During Handling. AU - Fernández-Pastor,Sandra, AU - Castelló,Dolores Silvestre, AU - López-Mendoza,M C, Y1 - 2020/06/24/ PY - 2020/6/25/entrez KW - breastfeeding KW - human milk KW - human milk collection KW - milk banking SP - 890334420932574 EP - 890334420932574 JF - Journal of human lactation : official journal of International Lactation Consultant Association JO - J Hum Lact N2 - BACKGROUND: Neonatal infections with Cronobacter sakazakii have recently been associated with the consumption of expressed human milk. STUDY AIMS: (1) To evaluate whether human milk has antimicrobial capacity against C. sakazakii and (2) to determine the stability of its capacity when it is subjected to various treatments. METHODS: The antimicrobial capacity of human milk against C. sakazakii was evaluated using an observational, cross-sectional, comparative design. Mature human milk samples (N = 29) were subjected to different treatments. After incubation at 37°C for 72 hr, samples were compared with fresh milk on the stability of their antimicrobial capacity. Two-way analysis of variance (ANOVA) was performed. RESULTS: In fresh milk, counts of C. sakazakii were reduced by 47.26% (SD = 6.74) compared to controls. In treated milk, reductions were: refrigeration at 4°C for 72 hr (M = 33.84, SD = 13.84), freezing at -20°C for 1, 2, and 3 months (M = 40.31, SD = 9.10; M = 35.96, SD = 9.39; M = 26.20, SD = 13.55, respectively), Holder pasteurization (M = 23.56, SD = 15.61), and human milk bank treatment with (M = 14.37, SD = 18.02) and without bovine fortifier (M = 3.70, SD = 23.83). There were significant differences (p < .05) between fresh and treated milk. CONCLUSIONS: Human milk has antimicrobial capacity against C. sakazakii. However, its capacity is negatively influenced by common preservation and hygienization methods. Milk should be stored refrigerated for a maximum of 72 hr or frozen for a short period of time. SN - 1552-5732 UR - https://www.unboundmedicine.com/medline/citation/32579054/Stability_of_the_Antimicrobial_Capacity_of_Human_Milk_Against_Cronobacter_Sakazakii_During_Handling L2 - https://journals.sagepub.com/doi/10.1177/0890334420932574?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -
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