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Detecting SARS-CoV-2 RNA in conjunctival secretions: Is it a valuable diagnostic method of COVID-19?
J Med Virol. 2020 Jun 24 [Online ahead of print]JM

Abstract

The main purpose of this study is to evaluate the presence of viral RNA of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in conjunctival swab specimen of coronavirus disease 2019 (COVID-19) patients with and without conjunctivitis to establish the diagnostic value of reverse transcription-polymerase chain reaction (RT-PCR) in each case and to describe its clinical characteristics. A cross-sectional study was conducted at the Hospital Clinico San Carlos of Madrid, Spain. Thirty-six subjects from the COVID admission unit with laboratory-confirmed SARS-CoV-2 infection were included. Conjunctival swabs were collected from 18 patients with conjunctivitis and 18 patients without conjunctivitis and RT-PCR was performed. Conjunctival swab was collected from both eyes of 36 patients (72 eyes), detecting SARS-CoV-2 RNA in conjunctival swab of two patients (5.5%). Among the 18 patients with conjunctivitis, only one of them (5.5%) showed positive results. Likewise, SARS-CoV-2 RNA was detected in one patient without conjunctivitis (5.5%). The mean age of the 36 patients was 67.9 years (range, 28-92 years) and the male-to-female ratio was 0.44 (16:20). The mean days since the onset of COVID-19 symptoms until conjunctivitis manifestation was 8 (range, 1-24 days). The mean duration of the conjunctivitis was 3 days (range, 1-7 days). SARS-CoV-2 RNA may be detected in conjunctival swabs of both patients with and without conjunctivitis. This study revealed the same rate of positive results amongst the group with and without conjunctivitis, suggesting that detecting SARS-CoV-2 in ocular fluids is not conditioned on the presence of conjunctivitis. The presence of SARS-CoV-2 RNA in ocular samples highlights the role of the eye as a possible route of transmission of the disease.

Authors+Show Affiliations

No affiliation info availableServicio de Oftalmología, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain.Department of Microbiology, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IsISSC), Madrid, Spain.Servicio de Oftalmología, Departamento de Inmunología, Oftalmología y ORL, Hospital Clínico San Carlos, Facultad de Medicina, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain.Department of Microbiology, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IsISSC), Madrid, Spain.Servicio de Oftalmología, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain.Department of Microbiology, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IsISSC), Madrid, Spain.Servicio de Oftalmología, Departamento de Inmunología, Oftalmología y ORL, Hospital Clínico San Carlos, Facultad de Medicina, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32579256

Citation

Güemes-Villahoz, Noemi, et al. "Detecting SARS-CoV-2 RNA in Conjunctival Secretions: Is It a Valuable Diagnostic Method of COVID-19?" Journal of Medical Virology, 2020.
Güemes-Villahoz N, Burgos-Blasco B, Arribi-Vilela A, et al. Detecting SARS-CoV-2 RNA in conjunctival secretions: Is it a valuable diagnostic method of COVID-19? J Med Virol. 2020.
Güemes-Villahoz, N., Burgos-Blasco, B., Arribi-Vilela, A., Arriola-Villalobos, P., Rico-Luna, C. M., Cuiña-Sardiña, R., Delgado-Iribarren, A., & García-Feijoó, J. (2020). Detecting SARS-CoV-2 RNA in conjunctival secretions: Is it a valuable diagnostic method of COVID-19? Journal of Medical Virology. https://doi.org/10.1002/jmv.26219
Güemes-Villahoz N, et al. Detecting SARS-CoV-2 RNA in Conjunctival Secretions: Is It a Valuable Diagnostic Method of COVID-19. J Med Virol. 2020 Jun 24; PubMed PMID: 32579256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detecting SARS-CoV-2 RNA in conjunctival secretions: Is it a valuable diagnostic method of COVID-19? AU - Güemes-Villahoz,Noemi, AU - Burgos-Blasco,Barbara, AU - Arribi-Vilela,Ana, AU - Arriola-Villalobos,Pedro, AU - Rico-Luna,Carla M, AU - Cuiña-Sardiña,Ricardo, AU - Delgado-Iribarren,Alberto, AU - García-Feijoó,Julián, Y1 - 2020/06/24/ PY - 2020/06/18/received PY - 2020/06/21/revised PY - 2020/06/22/accepted PY - 2020/6/25/pubmed PY - 2020/6/25/medline PY - 2020/6/25/entrez KW - COVID KW - PCR KW - conjunctivitis KW - coronavirus KW - diagnosis JF - Journal of medical virology JO - J. Med. Virol. N2 - The main purpose of this study is to evaluate the presence of viral RNA of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in conjunctival swab specimen of coronavirus disease 2019 (COVID-19) patients with and without conjunctivitis to establish the diagnostic value of reverse transcription-polymerase chain reaction (RT-PCR) in each case and to describe its clinical characteristics. A cross-sectional study was conducted at the Hospital Clinico San Carlos of Madrid, Spain. Thirty-six subjects from the COVID admission unit with laboratory-confirmed SARS-CoV-2 infection were included. Conjunctival swabs were collected from 18 patients with conjunctivitis and 18 patients without conjunctivitis and RT-PCR was performed. Conjunctival swab was collected from both eyes of 36 patients (72 eyes), detecting SARS-CoV-2 RNA in conjunctival swab of two patients (5.5%). Among the 18 patients with conjunctivitis, only one of them (5.5%) showed positive results. Likewise, SARS-CoV-2 RNA was detected in one patient without conjunctivitis (5.5%). The mean age of the 36 patients was 67.9 years (range, 28-92 years) and the male-to-female ratio was 0.44 (16:20). The mean days since the onset of COVID-19 symptoms until conjunctivitis manifestation was 8 (range, 1-24 days). The mean duration of the conjunctivitis was 3 days (range, 1-7 days). SARS-CoV-2 RNA may be detected in conjunctival swabs of both patients with and without conjunctivitis. This study revealed the same rate of positive results amongst the group with and without conjunctivitis, suggesting that detecting SARS-CoV-2 in ocular fluids is not conditioned on the presence of conjunctivitis. The presence of SARS-CoV-2 RNA in ocular samples highlights the role of the eye as a possible route of transmission of the disease. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/32579256/Detecting_SARS_CoV_2_RNA_in_conjunctival_secretions:_Is_it_a_valuable_diagnostic_method_of_COVID_19 L2 - https://doi.org/10.1002/jmv.26219 DB - PRIME DP - Unbound Medicine ER -