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Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using Citrobacter spp. MS5 Culture Supernatant.
Biomolecules. 2020 06 22; 10(6)B

Abstract

The present study described the extracellular synthesis of silver nanoparticles (AgNPs) using environmental bacterial isolate Citrobacter spp. MS5 culture supernatant. To our best knowledge, no previous study reported the biosynthesis of AgNPs using this bacterial isolate. The biosynthesized AgNPs were characterized using different techniques like UV-Vis spectroscopy, fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) equipped with energy dispersive X-ray (EDX). The analysis of UV-Vis spectra revealed absorption maxima at 415 nm due to surface plasmon resonance (SPR) indicated the formation of AgNPs and FTIR spectrum confirmed the participation of proteins molecule in AgNPs synthesis. XRD and EDX spectrum confirmed the metallic and crystalline nature of AgNPs. TEM and SEM showed spherical nanoparticles with a size range of 5-15 nm. The biosynthesized AgNPs showed effective independent as well as enhanced combined antibacterial activity against extended spectrum β-lactamase (ESBL) producing multidrug resistant Gram-negative bacteria. Further, effective antifungal activity of AgNPs was observed towards pathogenic Candida spp. The present study provides evidence for eco-friendly biosynthesis of well-characterized AgNPs and their potential antibacterial as well as antifungal activity.

Authors+Show Affiliations

Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India.Department of Medical Biotechnology, Yeungnam University, Gyeongsan, 712-749, Korea.Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India.Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India.Department of Medical Biotechnology, Yeungnam University, Gyeongsan, 712-749, Korea. Research Institute of Cell Culture, Yeungnam University, Gyeongsan 38541, Korea.Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32580522

Citation

Mondal, Aftab Hossain, et al. "Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using Citrobacter Spp. MS5 Culture Supernatant." Biomolecules, vol. 10, no. 6, 2020.
Mondal AH, Yadav D, Ali A, et al. Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using Citrobacter spp. MS5 Culture Supernatant. Biomolecules. 2020;10(6).
Mondal, A. H., Yadav, D., Ali, A., Khan, N., Jin, J. O., & Haq, Q. M. R. (2020). Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using Citrobacter spp. MS5 Culture Supernatant. Biomolecules, 10(6). https://doi.org/10.3390/biom10060944
Mondal AH, et al. Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using Citrobacter Spp. MS5 Culture Supernatant. Biomolecules. 2020 06 22;10(6) PubMed PMID: 32580522.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using Citrobacter spp. MS5 Culture Supernatant. AU - Mondal,Aftab Hossain, AU - Yadav,Dhananjay, AU - Ali,Asghar, AU - Khan,Neelofar, AU - Jin,Jun O, AU - Haq,Qazi Mohd Rizwanul, Y1 - 2020/06/22/ PY - 2020/05/10/received PY - 2020/06/19/revised PY - 2020/06/19/accepted PY - 2020/6/26/entrez PY - 2020/6/26/pubmed PY - 2021/4/13/medline KW - Citrobacter spp. MS5 KW - Gram-negative bacteria KW - antibacterial KW - antifungal KW - silver nanoparticles JF - Biomolecules JO - Biomolecules VL - 10 IS - 6 N2 - The present study described the extracellular synthesis of silver nanoparticles (AgNPs) using environmental bacterial isolate Citrobacter spp. MS5 culture supernatant. To our best knowledge, no previous study reported the biosynthesis of AgNPs using this bacterial isolate. The biosynthesized AgNPs were characterized using different techniques like UV-Vis spectroscopy, fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) equipped with energy dispersive X-ray (EDX). The analysis of UV-Vis spectra revealed absorption maxima at 415 nm due to surface plasmon resonance (SPR) indicated the formation of AgNPs and FTIR spectrum confirmed the participation of proteins molecule in AgNPs synthesis. XRD and EDX spectrum confirmed the metallic and crystalline nature of AgNPs. TEM and SEM showed spherical nanoparticles with a size range of 5-15 nm. The biosynthesized AgNPs showed effective independent as well as enhanced combined antibacterial activity against extended spectrum β-lactamase (ESBL) producing multidrug resistant Gram-negative bacteria. Further, effective antifungal activity of AgNPs was observed towards pathogenic Candida spp. The present study provides evidence for eco-friendly biosynthesis of well-characterized AgNPs and their potential antibacterial as well as antifungal activity. SN - 2218-273X UR - https://www.unboundmedicine.com/medline/citation/32580522/Anti_Bacterial_and_Anti_Candidal_Activity_of_Silver_Nanoparticles_Biosynthesized_Using_Citrobacter_spp__MS5_Culture_Supernatant_ L2 - https://www.mdpi.com/resolver?pii=biom10060944 DB - PRIME DP - Unbound Medicine ER -