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Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity.
BMB Rep. 2020 Jul; 53(7):341-348.BR

Abstract

The targeted nuclease clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has recently emerged as a prominent gene manipulation method. Because of its ease in programming targeted DNA/protein binding through RNA in a vast range of organisms, this prokaryotic defense system is a versatile tool with many applications in the research field as well as high potential in agricultural and clinical improvements. This review will present a brief history that led to its discovery and adaptation. We also present some of its restrictions, and modifications that have been performed to overcome such restrictions, focusing specifically on the most common CRISPR/Cas9 mediated non-homologous end joint repair. [BMB Reports 2020; 53(7): 341-348].

Authors+Show Affiliations

Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, Korea.Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, Korea.

Pub Type(s)

News

Language

eng

PubMed ID

32580834

Citation

Shin, Juhyun, and Jae-Wook Oh. "Development of CRISPR/Cas9 System for Targeted DNA Modifications and Recent Improvements in Modification Efficiency and Specificity." BMB Reports, vol. 53, no. 7, 2020, pp. 341-348.
Shin J, Oh JW. Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity. BMB Rep. 2020;53(7):341-348.
Shin, J., & Oh, J. W. (2020). Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity. BMB Reports, 53(7), 341-348.
Shin J, Oh JW. Development of CRISPR/Cas9 System for Targeted DNA Modifications and Recent Improvements in Modification Efficiency and Specificity. BMB Rep. 2020;53(7):341-348. PubMed PMID: 32580834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity. AU - Shin,Juhyun, AU - Oh,Jae-Wook, PY - 2020/03/31/received PY - 2020/6/26/pubmed PY - 2020/6/26/medline PY - 2020/6/26/entrez SP - 341 EP - 348 JF - BMB reports JO - BMB Rep VL - 53 IS - 7 N2 - The targeted nuclease clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has recently emerged as a prominent gene manipulation method. Because of its ease in programming targeted DNA/protein binding through RNA in a vast range of organisms, this prokaryotic defense system is a versatile tool with many applications in the research field as well as high potential in agricultural and clinical improvements. This review will present a brief history that led to its discovery and adaptation. We also present some of its restrictions, and modifications that have been performed to overcome such restrictions, focusing specifically on the most common CRISPR/Cas9 mediated non-homologous end joint repair. [BMB Reports 2020; 53(7): 341-348]. SN - 1976-670X UR - https://www.unboundmedicine.com/medline/citation/32580834/Development_of_CRISPR/Cas9_system_for_targeted_DNA_modifications_and_recent_improvements_in_modification_efficiency_and_specificity L2 - http://www.bmbreports.org/journal/view.html?volume=53&number=7&spage=341 DB - PRIME DP - Unbound Medicine ER -
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