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Comparative genomics and antimicrobial resistance profiling of Elizabethkingia isolates reveals nosocomial transmission and in vitro susceptibility to fluoroquinolones, tetracyclines and trimethoprim-sulfamethoxazole.
J Clin Microbiol. 2020 Jun 24 [Online ahead of print]JC

Abstract

The Elizabethkingia genus has gained global attention in recent years as a sporadic, worldwide, nosocomial pathogen. Elizabethkingia spp. are intrinsically multidrug resistant, primarily infect immunocompromised individuals, and are associated with high mortality (∼20-40%). Yet, gaps remain in our understanding of transmission, global strain relatedness, antimicrobial resistance and effective therapy. Over a 16-year period 22 clinical and six hospital environmental isolates were collected from Queensland, Australia. Identification using the MALDI-TOF MS (VITEK® MS) and whole-genome sequencing was compared with a global strain dataset. Phylogenomic reconstruction robustly identified 22 E. anophelis, three E. miricola, two E. meningoseptica and one E. bruuniana, most of which branched as unique lineages. Global analysisrevealed some Australian E. anophelis isolates are genetically closely related to strains identified from the USA, England and Asia. Comparative genomics of clinical and environmental strains identified evidence of nosocomial transmission in patients, indicating probable infection from a hospital reservoir. Furthermore, broth microdilution against 39 antimicrobials revealed almost ubiquitous resistance to aminoglycosides, carbapenems, cephalosporins and penicillins. Like other international strains, our isolates expressed susceptibility to minocycline and levofloxacin and the less common trimethoprim/sulfamethoxazole. Our study demonstrates important new insights into the genetic diversity, environmental persistence, transmission of and potential effective therapy for Australian Elizabethkingia species.

Authors+Show Affiliations

University of Queensland Centre for Clinical Research, Royal Brisbane and Woman's Hospital, Herston, Queensland, Australia. Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, Queensland, Australia. Sunshine Coast Health Institute, Birtinya, Queensland, Australia.Central Microbiology, Pathology Queensland, Queensland Health, Herston, Queensland, Australia.University of Queensland Centre for Clinical Research, Royal Brisbane and Woman's Hospital, Herston, Queensland, Australia.University of Queensland Centre for Clinical Research, Royal Brisbane and Woman's Hospital, Herston, Queensland, Australia.Central Microbiology, Pathology Queensland, Queensland Health, Herston, Queensland, Australia.University of Queensland Centre for Clinical Research, Royal Brisbane and Woman's Hospital, Herston, Queensland, Australia.Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, Queensland, Australia. Sunshine Coast Health Institute, Birtinya, Queensland, Australia.Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, Queensland, Australia. Sunshine Coast Health Institute, Birtinya, Queensland, Australia.University of Queensland Centre for Clinical Research, Royal Brisbane and Woman's Hospital, Herston, Queensland, Australia.University of Queensland Centre for Clinical Research, Royal Brisbane and Woman's Hospital, Herston, Queensland, Australia p.harris@uq.edu.au. Central Microbiology, Pathology Queensland, Queensland Health, Herston, Queensland, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32580952

Citation

Burnard, Delaney, et al. "Comparative Genomics and Antimicrobial Resistance Profiling of Elizabethkingia Isolates Reveals Nosocomial Transmission and in Vitro Susceptibility to Fluoroquinolones, Tetracyclines and Trimethoprim-sulfamethoxazole." Journal of Clinical Microbiology, 2020.
Burnard D, Gore L, Henderson A, et al. Comparative genomics and antimicrobial resistance profiling of Elizabethkingia isolates reveals nosocomial transmission and in vitro susceptibility to fluoroquinolones, tetracyclines and trimethoprim-sulfamethoxazole. J Clin Microbiol. 2020.
Burnard, D., Gore, L., Henderson, A., Ranasinghe, A., Bergh, H., Cottrell, K., Sarovich, D. S., Price, E. P., Paterson, D. L., & Harris, P. N. A. (2020). Comparative genomics and antimicrobial resistance profiling of Elizabethkingia isolates reveals nosocomial transmission and in vitro susceptibility to fluoroquinolones, tetracyclines and trimethoprim-sulfamethoxazole. Journal of Clinical Microbiology. https://doi.org/10.1128/JCM.00730-20
Burnard D, et al. Comparative Genomics and Antimicrobial Resistance Profiling of Elizabethkingia Isolates Reveals Nosocomial Transmission and in Vitro Susceptibility to Fluoroquinolones, Tetracyclines and Trimethoprim-sulfamethoxazole. J Clin Microbiol. 2020 Jun 24; PubMed PMID: 32580952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative genomics and antimicrobial resistance profiling of Elizabethkingia isolates reveals nosocomial transmission and in vitro susceptibility to fluoroquinolones, tetracyclines and trimethoprim-sulfamethoxazole. AU - Burnard,Delaney, AU - Gore,Letitia, AU - Henderson,Andrew, AU - Ranasinghe,Ama, AU - Bergh,Haakon, AU - Cottrell,Kyra, AU - Sarovich,Derek S, AU - Price,Erin P, AU - Paterson,David L, AU - Harris,Patrick N A, Y1 - 2020/06/24/ PY - 2020/6/26/entrez JF - Journal of clinical microbiology JO - J. Clin. Microbiol. N2 - The Elizabethkingia genus has gained global attention in recent years as a sporadic, worldwide, nosocomial pathogen. Elizabethkingia spp. are intrinsically multidrug resistant, primarily infect immunocompromised individuals, and are associated with high mortality (∼20-40%). Yet, gaps remain in our understanding of transmission, global strain relatedness, antimicrobial resistance and effective therapy. Over a 16-year period 22 clinical and six hospital environmental isolates were collected from Queensland, Australia. Identification using the MALDI-TOF MS (VITEK® MS) and whole-genome sequencing was compared with a global strain dataset. Phylogenomic reconstruction robustly identified 22 E. anophelis, three E. miricola, two E. meningoseptica and one E. bruuniana, most of which branched as unique lineages. Global analysisrevealed some Australian E. anophelis isolates are genetically closely related to strains identified from the USA, England and Asia. Comparative genomics of clinical and environmental strains identified evidence of nosocomial transmission in patients, indicating probable infection from a hospital reservoir. Furthermore, broth microdilution against 39 antimicrobials revealed almost ubiquitous resistance to aminoglycosides, carbapenems, cephalosporins and penicillins. Like other international strains, our isolates expressed susceptibility to minocycline and levofloxacin and the less common trimethoprim/sulfamethoxazole. Our study demonstrates important new insights into the genetic diversity, environmental persistence, transmission of and potential effective therapy for Australian Elizabethkingia species. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/32580952/Comparative_genomics_and_antimicrobial_resistance_profiling_of_Elizabethkingia_isolates_reveals_nosocomial_transmission_and_in_vitro_susceptibility_to_fluoroquinolones,_tetracyclines_and_trimethoprim-sulfamethoxazole L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=32580952 DB - PRIME DP - Unbound Medicine ER -
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