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RNA sequencing analysis of the human retina and associated ocular tissues.
Sci Data. 2020 06 24; 7(1):199.SD

Abstract

The retina is a stratified layer of sensory neurons lining the posterior portion of the eye. In humans, fine detail and color vision are enabled by the macula, a central region of the retina dense in cone photoreceptors (PRs). Achromatic low light and peripheral vision are facilitated by rod PRs found with increasing density outside the macula in the peripheral retina. The outer retina is nourished by choroidal blood flow regulated by a single layer of intervening retinal pigment epithelial (RPE) cells. Existing human retinal transcriptome projects have been critical for studying aspects of retinal development and disease however, there are currently no publicly available data sets accurately describing the aging human central retina, peripheral retina, and supporting RPE/choroid. Here we used Illumina RNA sequencing (RNA-seq) analysis to characterize the mRNA transcriptome of rod and cone PR-enriched human retina as well as supporting macular RPE/choroid tissue. These data will be valuable to the vision research community for characterizing global changes in gene expression in clinically relevant ocular tissues.

Authors+Show Affiliations

Department of Biology, James Madison University, Harrisonburg, VA, 22807, USA.Department of Biology, James Madison University, Harrisonburg, VA, 22807, USA.Department of Biology, James Madison University, Harrisonburg, VA, 22807, USA. enkera@jmu.edu. Center for Genome & Metagenome Studies, James Madison University, Harrisonburg, VA, 22807, USA. enkera@jmu.edu.

Pub Type(s)

Dataset
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32581312

Citation

Schumacker, Scott T., et al. "RNA Sequencing Analysis of the Human Retina and Associated Ocular Tissues." Scientific Data, vol. 7, no. 1, 2020, p. 199.
Schumacker ST, Coppage KR, Enke RA. RNA sequencing analysis of the human retina and associated ocular tissues. Sci Data. 2020;7(1):199.
Schumacker, S. T., Coppage, K. R., & Enke, R. A. (2020). RNA sequencing analysis of the human retina and associated ocular tissues. Scientific Data, 7(1), 199. https://doi.org/10.1038/s41597-020-0541-4
Schumacker ST, Coppage KR, Enke RA. RNA Sequencing Analysis of the Human Retina and Associated Ocular Tissues. Sci Data. 2020 06 24;7(1):199. PubMed PMID: 32581312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RNA sequencing analysis of the human retina and associated ocular tissues. AU - Schumacker,Scott T, AU - Coppage,Krista R, AU - Enke,Ray A, Y1 - 2020/06/24/ PY - 2019/10/08/received PY - 2020/06/02/accepted PY - 2020/6/26/entrez PY - 2020/6/26/pubmed PY - 2021/1/8/medline SP - 199 EP - 199 JF - Scientific data JO - Sci Data VL - 7 IS - 1 N2 - The retina is a stratified layer of sensory neurons lining the posterior portion of the eye. In humans, fine detail and color vision are enabled by the macula, a central region of the retina dense in cone photoreceptors (PRs). Achromatic low light and peripheral vision are facilitated by rod PRs found with increasing density outside the macula in the peripheral retina. The outer retina is nourished by choroidal blood flow regulated by a single layer of intervening retinal pigment epithelial (RPE) cells. Existing human retinal transcriptome projects have been critical for studying aspects of retinal development and disease however, there are currently no publicly available data sets accurately describing the aging human central retina, peripheral retina, and supporting RPE/choroid. Here we used Illumina RNA sequencing (RNA-seq) analysis to characterize the mRNA transcriptome of rod and cone PR-enriched human retina as well as supporting macular RPE/choroid tissue. These data will be valuable to the vision research community for characterizing global changes in gene expression in clinically relevant ocular tissues. SN - 2052-4463 UR - https://www.unboundmedicine.com/medline/citation/32581312/RNA_sequencing_analysis_of_the_human_retina_and_associated_ocular_tissues_ L2 - https://doi.org/10.1038/s41597-020-0541-4 DB - PRIME DP - Unbound Medicine ER -