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Synergistic antimicrobial action and effect of active chitosan-gelatin biopolymeric films containing Thymus vulgaris, Ocimum basilicum and Origanum majorana essential oils against Escherichia coli and Staphylococcus aureus.
Cell Mol Biol (Noisy-le-grand). 2020 Jun 25; 66(4):214-223.CM

Abstract

To replace synthetic preservatives and expand green consumption, several essential oils have been tested in foods and food packaging due to their antimicrobial properties. This study aimed to analyze the synergistic antimicrobial action of a chitosan-gelatin based active biopolymers with the addition of essential oils (EOs). The antimicrobial agents were tested against foodborne microorganisms Staphylococcus aureus and Escherichia coli strains. The antibacterial activity of Thymus vulgaris, Ocimum basilicum, Origanum majorana, and the synergistic interactions among them were assessed according to the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microdilution method. Chitosan-gelatin based active biopolymers were developed, and 23% (w/w) of each essential oil and combined oils were added. The antimicrobial effect of active films was measured using the disk diffusion method. Active films with the addition of essential oils have potential applications as active packaging agents, especially those that demonstrated inhibition zones. Combined EOs can be used to enhance the antimicrobial activity, ensuring reduced doses used in active packaging and decreasing the association with adverse sensory characteristics.

Authors+Show Affiliations

School of Food Engineering, University of Campinas. Zip code: 13083-862, Campinas, Sao Paulo, Brazil.

School of Food Engineering, University of Campinas. Zip code: 13083-862, Campinas, Sao Paulo, Brazil.

School of Food Engineering, University of Campinas. Zip code: 13083-862, Campinas, Sao Paulo, Brazil.

School of Food Engineering, University of Campinas. Zip code: 13083-862, Campinas, Sao Paulo, Brazil.

Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL, 35899, USA.

Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

School of Food Engineering, University of Campinas. Zip code: 13083-862, Campinas, Sao Paulo, Brazil.

MeSH

Anti-Infective AgentsChitosanDrug SynergismEscherichia coliGelatinMicrobial Sensitivity TestsOcimum basilicumOils, VolatileOriganumStaphylococcus aureusThymus Plant

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32583781

Citation

De Souza, Victoria Vilaça Martins Alencar, et al. "Synergistic Antimicrobial Action and Effect of Active Chitosan-gelatin Biopolymeric Films Containing Thymus Vulgaris, Ocimum Basilicum and Origanum Majorana Essential Oils Against Escherichia Coli and Staphylococcus Aureus." Cellular and Molecular Biology (Noisy-le-Grand, France), vol. 66, no. 4, 2020, pp. 214-223.

De Souza VVMA, Crippa BL, De Almeida JM, et al. Synergistic antimicrobial action and effect of active chitosan-gelatin biopolymeric films containing Thymus vulgaris, Ocimum basilicum and Origanum majorana essential oils against Escherichia coli and Staphylococcus aureus. Cell Mol Biol (Noisy-le-grand). 2020;66(4):214-223.

De Souza, V. V. M. A., Crippa, B. L., De Almeida, J. M., Iacuzio, R., Setzer, W. N., Sharifi-Rad, J., & Silva, N. C. C. (2020). Synergistic antimicrobial action and effect of active chitosan-gelatin biopolymeric films containing Thymus vulgaris, Ocimum basilicum and Origanum majorana essential oils against Escherichia coli and Staphylococcus aureus. Cellular and Molecular Biology (Noisy-le-Grand, France), 66(4), 214-223.

De Souza VVMA, et al. Synergistic Antimicrobial Action and Effect of Active Chitosan-gelatin Biopolymeric Films Containing Thymus Vulgaris, Ocimum Basilicum and Origanum Majorana Essential Oils Against Escherichia Coli and Staphylococcus Aureus. Cell Mol Biol (Noisy-le-grand). 2020 Jun 25;66(4):214-223. PubMed PMID: 32583781.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Synergistic antimicrobial action and effect of active chitosan-gelatin biopolymeric films containing Thymus vulgaris, Ocimum basilicum and Origanum majorana essential oils against Escherichia coli and Staphylococcus aureus. AU - De Souza,Victoria Vilaça Martins Alencar, AU - Crippa,Bruna Lourenço, AU - De Almeida,Jaqueline Milagres, AU - Iacuzio,Raiza, AU - Setzer,William N, AU - Sharifi-Rad,Javad, AU - Silva,Nathália Cristina Cirone, Y1 - 2020/06/25/ PY - 2020/06/20/received PY - 2020/6/26/entrez PY - 2020/6/26/pubmed PY - 2021/1/20/medline KW - Antimicrobial activity KW - Essential oil KW - Pathogenic bacteria KW - Synergy. SP - 214 EP - 223 JF - Cellular and molecular biology (Noisy-le-Grand, France) JO - Cell Mol Biol (Noisy-le-grand) VL - 66 IS - 4 N2 - To replace synthetic preservatives and expand green consumption, several essential oils have been tested in foods and food packaging due to their antimicrobial properties. This study aimed to analyze the synergistic antimicrobial action of a chitosan-gelatin based active biopolymers with the addition of essential oils (EOs). The antimicrobial agents were tested against foodborne microorganisms Staphylococcus aureus and Escherichia coli strains. The antibacterial activity of Thymus vulgaris, Ocimum basilicum, Origanum majorana, and the synergistic interactions among them were assessed according to the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microdilution method. Chitosan-gelatin based active biopolymers were developed, and 23% (w/w) of each essential oil and combined oils were added. The antimicrobial effect of active films was measured using the disk diffusion method. Active films with the addition of essential oils have potential applications as active packaging agents, especially those that demonstrated inhibition zones. Combined EOs can be used to enhance the antimicrobial activity, ensuring reduced doses used in active packaging and decreasing the association with adverse sensory characteristics. SN - 1165-158X UR - https://www.unboundmedicine.com/medline/citation/32583781/Synergistic_antimicrobial_action_and_effect_of_active_chitosan_gelatin_biopolymeric_films_containing_Thymus_vulgaris_Ocimum_basilicum_and_Origanum_majorana_essential_oils_against_Escherichia_coli_and_Staphylococcus_aureus_ DB - PRIME DP - Unbound Medicine ER -