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Disorders on cardiovascular parameters in rats and in human blood cells caused by Lachesis acrochorda snake venom.
Toxicon. 2020 Sep; 184:180-191.T

Abstract

In Colombia, Lachesis acrochorda causes 2-3% of all snake envenomations. The accidents promote a high mortality rate (90%) due to blood and cardiovascular complications. Here, the effects of the snake venom of L. acrochorda (SVLa) were analyzed on human blood cells and on cardiovascular parameters of rats. SVLa induced blood coagulation, as measured by the prothrombin time test, but did not reduce the cell viability of neutrophils and platelets evaluated by the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay and by the lactate dehydrogenase (LDH) enzyme assay. In fact, SVLa increased the absorbance in tests made with platelets subjected to the MTT assay. SVLa induced platelet aggregation whose magnitude was comparable to that of the positive control adenosine diphosphate (ADP), and occurred earlier with increasing SVLa concentration. Acetylsalicylic acid (ASA, a cyclooxygenase inhibitor) or clopidogrel (an ADP receptor blocker) inhibited the aggregating effect of SVLa. Inhibition of SVLa-elicited platelet aggregation also resulted from the treatment with disodium ethylenediaminetetraacetate (Na2-EDTA; metalloproteinase inhibitor) and with 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF, serine protease inhibitor). In isolated right atrium of rats, SVLa increased slightly, but significantly, the magnitude of the spontaneous contractions and, in isolated rat aorta, SVLa relaxed KCl- or phenylephrine-induced contractions. In vivo, SVLa induced hypotension and bradycardia in rats, with detection of hemorrhage in pulmonary and renal tissues. Altogether, under experimental conditions, SVLa induced blood coagulation, platelet aggregation, hypotension and bradycardia. Part of the effects presented here may be explained by the presence of snake venom metalloproteinases (SVMPs) and snake venom serine proteases (SVSPs), constituents of SVLa.

Authors+Show Affiliations

Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil; Facultad de Ciencias Naturales, Exactas y de la Educación, Departamento de Biología, Centro de Investigaciones Biomédicas-Bioterio, Grupo de Investigaciones Herpetológicas y Toxinológicas, Universidad del Cauca, Popayán, Colombia.Facultad de Ciencias Naturales, Exactas y de la Educación, Departamento de Biología, Centro de Investigaciones Biomédicas-Bioterio, Grupo de Investigaciones Herpetológicas y Toxinológicas, Universidad del Cauca, Popayán, Colombia.Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil.Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil.Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil.Centro de Estudos Farmacêuticos e Cosméticos, Faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil.Centro de Estudos Farmacêuticos e Cosméticos, Faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil.Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil.Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil.Laboratório de Toxinologia, Instituto Oswaldo Cruz, Fiocruz, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, 21040-900, Brazil.Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil.Centro de Estudos Farmacêuticos e Cosméticos, Faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil.Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal Do Ceará, Fortaleza, Ceará, Brazil. Electronic address: pjcmagal@ufc.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32585218

Citation

Angel-Camilo, Karen Leonor, et al. "Disorders On Cardiovascular Parameters in Rats and in Human Blood Cells Caused By Lachesis Acrochorda Snake Venom." Toxicon : Official Journal of the International Society On Toxinology, vol. 184, 2020, pp. 180-191.
Angel-Camilo KL, Guerrero-Vargas JA, Carvalho EF, et al. Disorders on cardiovascular parameters in rats and in human blood cells caused by Lachesis acrochorda snake venom. Toxicon. 2020;184:180-191.
Angel-Camilo, K. L., Guerrero-Vargas, J. A., Carvalho, E. F., Lima-Silva, K., de Siqueira, R. J. B., Freitas, L. B. N., Sousa, J. A. C., Mota, M. R. L., Santos, A. A. D., Neves-Ferreira, A. G. D. C., Havt, A., Leal, L. K. A. M., & Magalhães, P. J. C. (2020). Disorders on cardiovascular parameters in rats and in human blood cells caused by Lachesis acrochorda snake venom. Toxicon : Official Journal of the International Society On Toxinology, 184, 180-191. https://doi.org/10.1016/j.toxicon.2020.06.009
Angel-Camilo KL, et al. Disorders On Cardiovascular Parameters in Rats and in Human Blood Cells Caused By Lachesis Acrochorda Snake Venom. Toxicon. 2020;184:180-191. PubMed PMID: 32585218.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Disorders on cardiovascular parameters in rats and in human blood cells caused by Lachesis acrochorda snake venom. AU - Angel-Camilo,Karen Leonor, AU - Guerrero-Vargas,Jimmy Alexander, AU - Carvalho,Emanuella Feitosa de, AU - Lima-Silva,Karine, AU - de Siqueira,Rodrigo José Bezerra, AU - Freitas,Lyara Barbosa Nogueira, AU - Sousa,João Antônio Costa de, AU - Mota,Mario Rogério Lima, AU - Santos,Armênio Aguiar Dos, AU - Neves-Ferreira,Ana Gisele da Costa, AU - Havt,Alexandre, AU - Leal,Luzia Kalyne Almeida Moreira, AU - Magalhães,Pedro Jorge Caldas, Y1 - 2020/06/23/ PY - 2020/01/31/received PY - 2020/06/11/revised PY - 2020/06/15/accepted PY - 2020/6/26/pubmed PY - 2020/6/26/medline PY - 2020/6/26/entrez KW - Hypotension KW - Lachesis KW - Snake venom KW - Vasodilation KW - platelet Aggregation SP - 180 EP - 191 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 184 N2 - In Colombia, Lachesis acrochorda causes 2-3% of all snake envenomations. The accidents promote a high mortality rate (90%) due to blood and cardiovascular complications. Here, the effects of the snake venom of L. acrochorda (SVLa) were analyzed on human blood cells and on cardiovascular parameters of rats. SVLa induced blood coagulation, as measured by the prothrombin time test, but did not reduce the cell viability of neutrophils and platelets evaluated by the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay and by the lactate dehydrogenase (LDH) enzyme assay. In fact, SVLa increased the absorbance in tests made with platelets subjected to the MTT assay. SVLa induced platelet aggregation whose magnitude was comparable to that of the positive control adenosine diphosphate (ADP), and occurred earlier with increasing SVLa concentration. Acetylsalicylic acid (ASA, a cyclooxygenase inhibitor) or clopidogrel (an ADP receptor blocker) inhibited the aggregating effect of SVLa. Inhibition of SVLa-elicited platelet aggregation also resulted from the treatment with disodium ethylenediaminetetraacetate (Na2-EDTA; metalloproteinase inhibitor) and with 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF, serine protease inhibitor). In isolated right atrium of rats, SVLa increased slightly, but significantly, the magnitude of the spontaneous contractions and, in isolated rat aorta, SVLa relaxed KCl- or phenylephrine-induced contractions. In vivo, SVLa induced hypotension and bradycardia in rats, with detection of hemorrhage in pulmonary and renal tissues. Altogether, under experimental conditions, SVLa induced blood coagulation, platelet aggregation, hypotension and bradycardia. Part of the effects presented here may be explained by the presence of snake venom metalloproteinases (SVMPs) and snake venom serine proteases (SVSPs), constituents of SVLa. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/32585218/Disorders_on_cardiovascular_parameters_in_rats_and_in_human_blood_cells_caused_by_Lachesis_acrochorda_snake_venom L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(20)30288-9 DB - PRIME DP - Unbound Medicine ER -
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