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Circulating Inflammatory miRNAs Associated with Parkinson's Disease Pathophysiology.
Biomolecules. 2020 06 23; 10(6)B

Abstract

Parkinson's disease (PD) is the second most common neurodegenerative disease worldwide, being largely characterized by motor features. MicroRNAs (miRNAs) are small non-coding RNAs, whose deregulation has been associated with neurodegeneration in PD. In this study, miRNAs targeting cell death and/or inflammation pathways were selected and their expression compared in the serum of PD patients and healthy controls. We used two independent cohorts (discovery and validation) of 20 idiopathic PD patients (iPD) and 20 healthy controls each. We also analyzed an additional group of 45 patients with a mutation in the leucine-rich repeat kinase 2 (LRRK2) gene (LRRK2-PD). miRNA expression was determined using Taqman qRT-PCR and their performance to discriminate between groups was assessed by receiver operating characteristic (ROC) curve analysis. We found miR-146a, miR-335-3p, and miR-335-5p downregulated in iPD and LRRK2-PD patients versus controls in both cohorts. In addition, miR-155 was upregulated in LRRK2-PD compared to iPD patients showing an appropriate value of area under the ROC curve (AUC 0.80) to discriminate between the two groups. In conclusion, our study identified a panel of inflammatory related miRNAs differentially expressed between PD patients and healthy controls that highlight key pathophysiological processes and may contribute to improve disease diagnosis.

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Authors+Show Affiliations

Oliveira SR
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, Portugal.
Dionísio PA
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, Portugal.
Correia Guedes L
Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal. Department of Neuroscience and Mental Health, Neurology, Hospital de Santa Maria, Centro Hospitalar Universitário Lisboa Norte, 1600-190 Lisbon, Portugal.
Gonçalves N
Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal.
Coelho M
Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal. Department of Neuroscience and Mental Health, Neurology, Hospital de Santa Maria, Centro Hospitalar Universitário Lisboa Norte, 1600-190 Lisbon, Portugal.
Rosa MM
Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal. Department of Neuroscience and Mental Health, Neurology, Hospital de Santa Maria, Centro Hospitalar Universitário Lisboa Norte, 1600-190 Lisbon, Portugal. Laboratory of Clinical Pharmacology and Therapeutics, Faculdade de Medicina, Universidade de Lisboa, 1600-190 Lisbon, Portugal.
Amaral JD
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, Portugal.
Ferreira JJ
Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisbon, Portugal. Laboratory of Clinical Pharmacology and Therapeutics, Faculdade de Medicina, Universidade de Lisboa, 1600-190 Lisbon, Portugal.
Rodrigues CMP
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, Portugal.

MeSH

AgedCase-Control StudiesCohort StudiesFemaleHumansInflammationMaleMicroRNAsParkinson Disease

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32585840

Citation

Oliveira, Sara R., et al. "Circulating Inflammatory miRNAs Associated With Parkinson's Disease Pathophysiology." Biomolecules, vol. 10, no. 6, 2020.
Oliveira SR, Dionísio PA, Correia Guedes L, et al. Circulating Inflammatory miRNAs Associated with Parkinson's Disease Pathophysiology. Biomolecules. 2020;10(6).
Oliveira, S. R., Dionísio, P. A., Correia Guedes, L., Gonçalves, N., Coelho, M., Rosa, M. M., Amaral, J. D., Ferreira, J. J., & Rodrigues, C. M. P. (2020). Circulating Inflammatory miRNAs Associated with Parkinson's Disease Pathophysiology. Biomolecules, 10(6). https://doi.org/10.3390/biom10060945
Oliveira SR, et al. Circulating Inflammatory miRNAs Associated With Parkinson's Disease Pathophysiology. Biomolecules. 2020 06 23;10(6) PubMed PMID: 32585840.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circulating Inflammatory miRNAs Associated with Parkinson's Disease Pathophysiology. AU - Oliveira,Sara R, AU - Dionísio,Pedro A, AU - Correia Guedes,Leonor, AU - Gonçalves,Nilza, AU - Coelho,Miguel, AU - Rosa,Mário M, AU - Amaral,Joana D, AU - Ferreira,Joaquim J, AU - Rodrigues,Cecília M P, Y1 - 2020/06/23/ PY - 2020/05/08/received PY - 2020/06/16/revised PY - 2020/06/17/accepted PY - 2020/6/27/entrez PY - 2020/6/27/pubmed PY - 2021/4/13/medline KW - LRRK2 KW - Parkinson’s disease KW - inflammatory miRNAs KW - serum miRNAs JF - Biomolecules JO - Biomolecules VL - 10 IS - 6 N2 - Parkinson's disease (PD) is the second most common neurodegenerative disease worldwide, being largely characterized by motor features. MicroRNAs (miRNAs) are small non-coding RNAs, whose deregulation has been associated with neurodegeneration in PD. In this study, miRNAs targeting cell death and/or inflammation pathways were selected and their expression compared in the serum of PD patients and healthy controls. We used two independent cohorts (discovery and validation) of 20 idiopathic PD patients (iPD) and 20 healthy controls each. We also analyzed an additional group of 45 patients with a mutation in the leucine-rich repeat kinase 2 (LRRK2) gene (LRRK2-PD). miRNA expression was determined using Taqman qRT-PCR and their performance to discriminate between groups was assessed by receiver operating characteristic (ROC) curve analysis. We found miR-146a, miR-335-3p, and miR-335-5p downregulated in iPD and LRRK2-PD patients versus controls in both cohorts. In addition, miR-155 was upregulated in LRRK2-PD compared to iPD patients showing an appropriate value of area under the ROC curve (AUC 0.80) to discriminate between the two groups. In conclusion, our study identified a panel of inflammatory related miRNAs differentially expressed between PD patients and healthy controls that highlight key pathophysiological processes and may contribute to improve disease diagnosis. SN - 2218-273X UR - https://www.unboundmedicine.com/medline/citation/32585840/Circulating_Inflammatory_miRNAs_Associated_with_Parkinson's_Disease_Pathophysiology_ DB - PRIME DP - Unbound Medicine ER -