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Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats.
Pain Res Manag. 2020; 2020:7531409.PR

Abstract

Objective

To study the effects of the three methods and three-acupoint technique on DRG gene expression in SNI model rats and to elucidate the molecular mechanism of the three methods and three-acupoint technique on promoting recovery in peripheral nerve injury.

Methods

27 male SD rats were randomly divided into three groups: a Sham group, the SNI group, and the Tuina group. The Tuina group was treated with a tuina manipulation simulator to simulate massage on points, controlling for both quality and quantity. Point-pressing, plucking, and kneading methods were administered quantitatively at Yinmen (BL37), Chengshan (BL57), and Yanglingquan (GB34) points on the affected side once a day, beginning 7 days after modeling. Intervention was applied once a day for 10 days, then 1 day of rest, followed by 10 more days of intervention, totally equaling 20 times of intervention. The effect of the three methods and three-point technique on the recovery of injured rats was evaluated using behavior analysis. RNA sequencing (RNA-Seq) analysis of differentially expressed genes in DRGs of the three groups of rats was also performed. GO and KEGG enrichment was analyzed and verified using real-time PCR.

Results

RNA-Seq combined with database information showed that the number of differentially expressed genes in DRG was the largest in the Tuina group compared with the SNI group, totaling 226. GO function is enriched in the positive regulation of cell processes, ion binding, protein binding, neuron, response to pressure, response to metal ions, neuron projection, and other biological processes. GO function is also enriched in the Wnt, IL-17, and MAPK signaling pathways in the KEGG database. PCR results were consistent with those of RNA sequencing, suggesting that the results of transcriptome sequencing were reliable.

Conclusion

The three methods and three-acupoint technique can promote the recovery of SNI model rats by altering the gene sequence in DRGs.

Authors+Show Affiliations

School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32587647

Citation

Lv, Tao-Tao, et al. "Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique On DRGs in Sciatic Nerve Injured Rats." Pain Research & Management, vol. 2020, 2020, p. 7531409.
Lv TT, Mo YJ, Yu TY, et al. Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats. Pain Res Manag. 2020;2020:7531409.
Lv, T. T., Mo, Y. J., Yu, T. Y., Shao, S., Lu, M. Q., Luo, Y. T., Shen, Y., Zhang, Y. M., & Steven, W. (2020). Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats. Pain Research & Management, 2020, 7531409. https://doi.org/10.1155/2020/7531409
Lv TT, et al. Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique On DRGs in Sciatic Nerve Injured Rats. Pain Res Manag. 2020;2020:7531409. PubMed PMID: 32587647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats. AU - Lv,Tao-Tao, AU - Mo,Yan-Jun, AU - Yu,Tian-Yuan, AU - Shao,Shuai, AU - Lu,Meng-Qian, AU - Luo,Yu-Ting, AU - Shen,Yi, AU - Zhang,Yu-Mo, AU - Steven,Wong, Y1 - 2020/06/07/ PY - 2019/11/06/received PY - 2020/01/31/accepted PY - 2020/6/27/entrez PY - 2020/6/27/pubmed PY - 2020/6/27/medline SP - 7531409 EP - 7531409 JF - Pain research & management JO - Pain Res Manag VL - 2020 N2 - Objective: To study the effects of the three methods and three-acupoint technique on DRG gene expression in SNI model rats and to elucidate the molecular mechanism of the three methods and three-acupoint technique on promoting recovery in peripheral nerve injury. Methods: 27 male SD rats were randomly divided into three groups: a Sham group, the SNI group, and the Tuina group. The Tuina group was treated with a tuina manipulation simulator to simulate massage on points, controlling for both quality and quantity. Point-pressing, plucking, and kneading methods were administered quantitatively at Yinmen (BL37), Chengshan (BL57), and Yanglingquan (GB34) points on the affected side once a day, beginning 7 days after modeling. Intervention was applied once a day for 10 days, then 1 day of rest, followed by 10 more days of intervention, totally equaling 20 times of intervention. The effect of the three methods and three-point technique on the recovery of injured rats was evaluated using behavior analysis. RNA sequencing (RNA-Seq) analysis of differentially expressed genes in DRGs of the three groups of rats was also performed. GO and KEGG enrichment was analyzed and verified using real-time PCR. Results: RNA-Seq combined with database information showed that the number of differentially expressed genes in DRG was the largest in the Tuina group compared with the SNI group, totaling 226. GO function is enriched in the positive regulation of cell processes, ion binding, protein binding, neuron, response to pressure, response to metal ions, neuron projection, and other biological processes. GO function is also enriched in the Wnt, IL-17, and MAPK signaling pathways in the KEGG database. PCR results were consistent with those of RNA sequencing, suggesting that the results of transcriptome sequencing were reliable. Conclusion: The three methods and three-acupoint technique can promote the recovery of SNI model rats by altering the gene sequence in DRGs. SN - 1918-1523 UR - https://www.unboundmedicine.com/medline/citation/32587647/Using_RNA-Seq_to_Explore_the_Repair_Mechanism_of_the_Three_Methods_and_Three-Acupoint_Technique_on_DRGs_in_Sciatic_Nerve_Injured_Rats L2 - https://doi.org/10.1155/2020/7531409 DB - PRIME DP - Unbound Medicine ER -
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