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Ocular Surface Expression of SARS-CoV-2 Receptors.
Ocul Immunol Inflamm. 2020 Jul 03; 28(5):735-738.OI

Abstract

PURPOSE

The spike proteins of SARS-CoV-2 interact with ACE2 or basigin/CD147 receptors, regulating human-to-human transmissions of COVID-19 together with serine protease TMPRSS2. The expression of these receptors on the ocular surface is unknown.

MATERIAL AND METHODS

Gene expression of SARS-CoV-2 receptors was investigated in conjunctival epithelial cell samples and in ex-vivo cornea samples using microarray or transcriptome sequencing.

RESULTS

ACE2 is expressed in conjunctival samples at a low level, while BSG and TMPRSS2 are expressed at intermediate levels in both conjunctiva and cornea. Other receptors such as ANPEP, AGTR2 are expressed at low level in the conjunctiva. Two RNA editing enzymes involved in antiviral responses, APOBEC3A, and ADAR-1 were also highly expressed.

CONCLUSIONS

The ocular surface may represent an entry point for the SARS-CoV-2 in the human body. The conjunctiva and the cornea can adopt antiviral countermeasures which may explain the low prevalence of eye involvement.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Leonardi A
Department of Neuroscience, Ophthalmology Unit, University of Padova , Padova, Italy.
Rosani U
Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research , List, Germany.
Brun P
Department of Molecular Medicine, Histology Unit, University of Padova , Padova, Italy.

MeSH

Adenosine DeaminaseAdolescentAdultAgedAngiotensin-Converting Enzyme 2BasiginBetacoronavirusCOVID-19ChildConjunctivaCorneaCoronavirus InfectionsCytidine DeaminaseGene Expression ProfilingGene Expression RegulationHumansMiddle AgedPandemicsPeptidyl-Dipeptidase APneumonia, ViralProteinsRNA-Binding ProteinsReceptors, VirusSARS-CoV-2Serine EndopeptidasesYoung Adult

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32589459

Citation

Leonardi, Andrea, et al. "Ocular Surface Expression of SARS-CoV-2 Receptors." Ocular Immunology and Inflammation, vol. 28, no. 5, 2020, pp. 735-738.
Leonardi A, Rosani U, Brun P. Ocular Surface Expression of SARS-CoV-2 Receptors. Ocul Immunol Inflamm. 2020;28(5):735-738.
Leonardi, A., Rosani, U., & Brun, P. (2020). Ocular Surface Expression of SARS-CoV-2 Receptors. Ocular Immunology and Inflammation, 28(5), 735-738. https://doi.org/10.1080/09273948.2020.1772314
Leonardi A, Rosani U, Brun P. Ocular Surface Expression of SARS-CoV-2 Receptors. Ocul Immunol Inflamm. 2020 Jul 3;28(5):735-738. PubMed PMID: 32589459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ocular Surface Expression of SARS-CoV-2 Receptors. AU - Leonardi,Andrea, AU - Rosani,Umberto, AU - Brun,Paola, Y1 - 2020/06/26/ PY - 2020/6/27/pubmed PY - 2020/8/7/medline PY - 2020/6/27/entrez KW - ACE2 KW - ADAR KW - APOBEC3 KW - CD147 KW - COVID-19 KW - SARS-CoV-2 KW - TMPRSS2 KW - conjunctiva KW - cornea SP - 735 EP - 738 JF - Ocular immunology and inflammation JO - Ocul Immunol Inflamm VL - 28 IS - 5 N2 - PURPOSE: The spike proteins of SARS-CoV-2 interact with ACE2 or basigin/CD147 receptors, regulating human-to-human transmissions of COVID-19 together with serine protease TMPRSS2. The expression of these receptors on the ocular surface is unknown. MATERIAL AND METHODS: Gene expression of SARS-CoV-2 receptors was investigated in conjunctival epithelial cell samples and in ex-vivo cornea samples using microarray or transcriptome sequencing. RESULTS: ACE2 is expressed in conjunctival samples at a low level, while BSG and TMPRSS2 are expressed at intermediate levels in both conjunctiva and cornea. Other receptors such as ANPEP, AGTR2 are expressed at low level in the conjunctiva. Two RNA editing enzymes involved in antiviral responses, APOBEC3A, and ADAR-1 were also highly expressed. CONCLUSIONS: The ocular surface may represent an entry point for the SARS-CoV-2 in the human body. The conjunctiva and the cornea can adopt antiviral countermeasures which may explain the low prevalence of eye involvement. SN - 1744-5078 UR - https://www.unboundmedicine.com/medline/citation/32589459/Ocular_Surface_Expression_of_SARS_CoV_2_Receptors_ DB - PRIME DP - Unbound Medicine ER -