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Phenotype and kinetics of SARS-CoV-2-specific T cells in COVID-19 patients with acute respiratory distress syndrome.
Sci Immunol. 2020 06 26; 5(48)SI

Abstract

SARS-CoV-2 has been identified as the causative agent of a global outbreak of respiratory tract disease (COVID-19). In some patients the infection results in moderate to severe acute respiratory distress syndrome (ARDS), requiring invasive mechanical ventilation. High serum levels of IL-6, IL-10 and an immune hyperresponsiveness referred to as a 'cytokine storm' have been associated with poor clinical outcome. Despite the large numbers of COVID-19 cases and deaths, information on the phenotype and kinetics of SARS-CoV-2-specific T cells is limited. Here, we studied 10 COVID-19 patients who required admission to an intensive care unit and detected SARS-CoV-2-specific CD4+ and CD8+ T cells in 10 out of 10 and 8 out of 10 patients, respectively. We also detected low levels of SARS-CoV-2-reactive T cells in 2 out of 10 healthy controls not previously exposed to SARS-CoV-2, which is indicative of cross-reactivity due to past infection with 'common cold' coronaviruses. The strongest T-cell responses were directed to the spike (S) surface glycoprotein, and SARS-CoV-2-specific T cells predominantly produced effector and Th1 cytokines, although Th2 and Th17 cytokines were also detected. Furthermore, we studied T-cell kinetics and showed that SARS-CoV-2-specific T cells are present relatively early and increase over time. Collectively, these data shed light on the potential variations in T-cell responses as a function of disease severity, an issue that is key to understanding the potential role of immunopathology in the disease, and also inform vaccine design and evaluation.

Authors+Show Affiliations

Center for Infectious Disease, La Jolla Institute for Immunology, La Jolla, CA 92037, USA.Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.Center for Infectious Disease, La Jolla Institute for Immunology, La Jolla, CA 92037, USA.Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.Department of Intensive Care, Erasmus MC, Rotterdam, the Netherlands.Department of Intensive Care, Erasmus MC, Rotterdam, the Netherlands.Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.Center for Infectious Disease, La Jolla Institute for Immunology, La Jolla, CA 92037, USA. Department of Pathology, University of California, San Diego, CA 92037, USA. Department of Medicine, University of California, San Diego, CA 92037, USA.Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands. r.d.devries@erasmusmc.nl.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32591408

Citation

Weiskopf, Daniela, et al. "Phenotype and Kinetics of SARS-CoV-2-specific T Cells in COVID-19 Patients With Acute Respiratory Distress Syndrome." Science Immunology, vol. 5, no. 48, 2020.
Weiskopf D, Schmitz KS, Raadsen MP, et al. Phenotype and kinetics of SARS-CoV-2-specific T cells in COVID-19 patients with acute respiratory distress syndrome. Sci Immunol. 2020;5(48).
Weiskopf, D., Schmitz, K. S., Raadsen, M. P., Grifoni, A., Okba, N. M. A., Endeman, H., van den Akker, J. P. C., Molenkamp, R., Koopmans, M. P. G., van Gorp, E. C. M., Haagmans, B. L., de Swart, R. L., Sette, A., & de Vries, R. D. (2020). Phenotype and kinetics of SARS-CoV-2-specific T cells in COVID-19 patients with acute respiratory distress syndrome. Science Immunology, 5(48). https://doi.org/10.1126/sciimmunol.abd2071
Weiskopf D, et al. Phenotype and Kinetics of SARS-CoV-2-specific T Cells in COVID-19 Patients With Acute Respiratory Distress Syndrome. Sci Immunol. 2020 06 26;5(48) PubMed PMID: 32591408.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotype and kinetics of SARS-CoV-2-specific T cells in COVID-19 patients with acute respiratory distress syndrome. AU - Weiskopf,Daniela, AU - Schmitz,Katharina S, AU - Raadsen,Matthijs P, AU - Grifoni,Alba, AU - Okba,Nisreen M A, AU - Endeman,Henrik, AU - van den Akker,Johannes P C, AU - Molenkamp,Richard, AU - Koopmans,Marion P G, AU - van Gorp,Eric C M, AU - Haagmans,Bart L, AU - de Swart,Rik L, AU - Sette,Alessandro, AU - de Vries,Rory D, PY - 2020/06/08/received PY - 2020/06/23/accepted PY - 2020/6/28/entrez PY - 2020/6/28/pubmed PY - 2020/7/16/medline JF - Science immunology JO - Sci Immunol VL - 5 IS - 48 N2 - SARS-CoV-2 has been identified as the causative agent of a global outbreak of respiratory tract disease (COVID-19). In some patients the infection results in moderate to severe acute respiratory distress syndrome (ARDS), requiring invasive mechanical ventilation. High serum levels of IL-6, IL-10 and an immune hyperresponsiveness referred to as a 'cytokine storm' have been associated with poor clinical outcome. Despite the large numbers of COVID-19 cases and deaths, information on the phenotype and kinetics of SARS-CoV-2-specific T cells is limited. Here, we studied 10 COVID-19 patients who required admission to an intensive care unit and detected SARS-CoV-2-specific CD4+ and CD8+ T cells in 10 out of 10 and 8 out of 10 patients, respectively. We also detected low levels of SARS-CoV-2-reactive T cells in 2 out of 10 healthy controls not previously exposed to SARS-CoV-2, which is indicative of cross-reactivity due to past infection with 'common cold' coronaviruses. The strongest T-cell responses were directed to the spike (S) surface glycoprotein, and SARS-CoV-2-specific T cells predominantly produced effector and Th1 cytokines, although Th2 and Th17 cytokines were also detected. Furthermore, we studied T-cell kinetics and showed that SARS-CoV-2-specific T cells are present relatively early and increase over time. Collectively, these data shed light on the potential variations in T-cell responses as a function of disease severity, an issue that is key to understanding the potential role of immunopathology in the disease, and also inform vaccine design and evaluation. SN - 2470-9468 UR - https://www.unboundmedicine.com/medline/citation/32591408/Phenotype_and_kinetics_of_SARS_CoV_2_specific_T_cells_in_COVID_19_patients_with_acute_respiratory_distress_syndrome_ L2 - https://immunology.sciencemag.org/cgi/pmidlookup?view=long&pmid=32591408 DB - PRIME DP - Unbound Medicine ER -