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SIX1 and SIX4 homeoproteins regulate PAX7+ progenitor cell properties during fetal epaxial myogenesis.
Development. 2020 Jun 26 [Online ahead of print]D

Abstract

Pax7 expression marks stem cells in developing skeletal muscles and adult satellite cells during homeostasis and muscle regeneration. The genetic determinants that control the entrance into the myogenic program and the appearance of PAX7+ cells during embryogenesis are poorly understood. SIX homeoproteins are encoded by the Sine oculis homeobox related Six1-Six6 genes in vertebrates. Six1, Six2, Six4 and Six5 are expressed in the muscle lineage. Here we tested the hypothesis that Six1 and Six4 could participate in the genesis of myogenic stem cells. We show that fewer PAX7+ cells occupy a satellite cell position between the myofiber and its associated basal lamina in Six1 and Six4 (s1s4KO) at E18. However, PAX7+ cells are detected in remaining muscle masses present in the epaxial region of the double mutant embryos and are able to divide and contribute to muscle growth. To further characterize the properties of s1s4KO PAX7+ cells, we analyzed their transcriptome and tested their properties after transplantation in adult regenerating tibialis anterior (TA) muscle. Mutant stem cells form hypotrophic myofibers that are not innervated but retain the ability to self-renew.

Authors+Show Affiliations

Université de Paris, Institut Cochin, INSERM, CNRS, F-75014 PARIS, France.Université de Paris, Institut Cochin, INSERM, CNRS, F-75014 PARIS, France.Université de Paris, Institut Cochin, INSERM, CNRS, F-75014 PARIS, France.Proteo-Science Center, Ehime University, Toon, Ehime 791-0295, Japan. Stem Cells and Development, Institut Pasteur, 75015, Paris, France. CNRS UMR 3738, Institut Pasteur, Paris, France.Sorbonne Université, Institut de Myologie, INSERM, 75013 Paris, France.Université de Paris, Institut Cochin, INSERM, CNRS, F-75014 PARIS, France.Université de Paris, Institut Cochin, INSERM, CNRS, F-75014 PARIS, France.Sorbonne Université, Institut de Myologie, INSERM, 75013 Paris, France.INSERM U1179, LIA BAHN CSM, Université de Versailles Saint-Quentin-en-Yvelines, Montigny-le-Bretonneux, France.Fred Hutchinson Cancer Research Center, Seattle, USA.Max Delbrück Center for Molecular Medicine, Berlin, Germany.Stem Cells and Development, Institut Pasteur, 75015, Paris, France. CNRS UMR 3738, Institut Pasteur, Paris, France.Université de Paris, Institut Cochin, INSERM, CNRS, F-75014 PARIS, France. Institut NeuroMyoGène, Université Claude Bernard Lyon 1, CNRS, INSERM, 69008 Lyon, France.Université de Paris, Institut Cochin, INSERM, CNRS, F-75014 PARIS, France.Université de Paris, Institut Cochin, INSERM, CNRS, F-75014 PARIS, France pascal.maire@inserm.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32591430

Citation

Wurmser, Maud, et al. "SIX1 and SIX4 Homeoproteins Regulate PAX7+ Progenitor Cell Properties During Fetal Epaxial Myogenesis." Development (Cambridge, England), 2020.
Wurmser M, Chaverot N, Madani R, et al. SIX1 and SIX4 homeoproteins regulate PAX7+ progenitor cell properties during fetal epaxial myogenesis. Development. 2020.
Wurmser, M., Chaverot, N., Madani, R., Sakai, H., Negroni, E., Demignon, J., Saint-Pierre, B., Mouly, V., Amthor, H., Tapscott, S., Birchmeier, C., Tajbakhsh, S., Le Grand, F., Sotiropoulos, A., & Maire, P. (2020). SIX1 and SIX4 homeoproteins regulate PAX7+ progenitor cell properties during fetal epaxial myogenesis. Development (Cambridge, England). https://doi.org/10.1242/dev.185975
Wurmser M, et al. SIX1 and SIX4 Homeoproteins Regulate PAX7+ Progenitor Cell Properties During Fetal Epaxial Myogenesis. Development. 2020 Jun 26; PubMed PMID: 32591430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SIX1 and SIX4 homeoproteins regulate PAX7+ progenitor cell properties during fetal epaxial myogenesis. AU - Wurmser,Maud, AU - Chaverot,Nathalie, AU - Madani,Rouba, AU - Sakai,Hiroshi, AU - Negroni,Elisa, AU - Demignon,Josiane, AU - Saint-Pierre,Benjamin, AU - Mouly,Vincent, AU - Amthor,Helge, AU - Tapscott,Stephen, AU - Birchmeier,Carmen, AU - Tajbakhsh,Shahragim, AU - Le Grand,Fabien, AU - Sotiropoulos,Athanassia, AU - Maire,Pascal, Y1 - 2020/06/26/ PY - 2019/11/08/received PY - 2020/06/18/accepted PY - 2020/6/28/entrez KW - Homing KW - Pax7 KW - Six1 KW - Six4 KW - Stem cells JF - Development (Cambridge, England) JO - Development N2 - Pax7 expression marks stem cells in developing skeletal muscles and adult satellite cells during homeostasis and muscle regeneration. The genetic determinants that control the entrance into the myogenic program and the appearance of PAX7+ cells during embryogenesis are poorly understood. SIX homeoproteins are encoded by the Sine oculis homeobox related Six1-Six6 genes in vertebrates. Six1, Six2, Six4 and Six5 are expressed in the muscle lineage. Here we tested the hypothesis that Six1 and Six4 could participate in the genesis of myogenic stem cells. We show that fewer PAX7+ cells occupy a satellite cell position between the myofiber and its associated basal lamina in Six1 and Six4 (s1s4KO) at E18. However, PAX7+ cells are detected in remaining muscle masses present in the epaxial region of the double mutant embryos and are able to divide and contribute to muscle growth. To further characterize the properties of s1s4KO PAX7+ cells, we analyzed their transcriptome and tested their properties after transplantation in adult regenerating tibialis anterior (TA) muscle. Mutant stem cells form hypotrophic myofibers that are not innervated but retain the ability to self-renew. SN - 1477-9129 UR - https://www.unboundmedicine.com/medline/citation/32591430/SIX1_and_SIX4_homeoproteins_regulate_PAX7+_progenitor_cell_properties_during_fetal_epaxial_myogenesis L2 - http://dev.biologists.org/cgi/pmidlookup?view=long&pmid=32591430 DB - PRIME DP - Unbound Medicine ER -
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