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Decreased Expression of Heat Shock Protein 47 Is Associated with T-2 Toxin and Low Selenium-Induced Matrix Degradation in Cartilages of Kashin-Beck Disease.
Biol Trace Elem Res. 2020 Jun 26 [Online ahead of print]BT

Abstract

Recent evidence suggests a role of type II collagen in Kashin-Beck disease (KBD) degeneration. We aimed to assess the abnormal expression of heat shock protein 47 (HSP47) which is associated with a decrease in type II collagen and an increase in cartilage degradation in KBD. Hand phalange cartilages were collected from KBD and healthy children. Rats were administered with T-2 toxin under the selenium (Se)-deficient diet. ATDC5 cells were seeded on bone matrix gelatin to construct engineered cartilaginous tissue. C28/I2 and ATDC5 cells and engineered tissue were exposed to different concentrations of T-2 toxin with or without Se. Cartilage degeneration was determined through histological evaluation. The distribution and expression of type II collagen and HSP47 were investigated through immunohistochemistry, western blotting, and real-time PCR. KBD cartilages showed increased chondronecrosis and extracellular matrix degradation in deep zone with decreased type II collagen and HSP47 expression. The low-Se + T-2 toxin animal group showed a significantly lower type II collagen expression along with decreased HSP47 expression. Decreased type II collagen and HSP47 in C28/I2 and ATDC5 cells induced by T-2 toxin showed a dose-dependent manner. Hyaline-like cartilage with zonal layers was developed in engineered cartilaginous tissues, with decreased type II collagen and HSP47 expression found in T-2 toxin-treated group. Se-supplementation partially antagonized the inhibitory effects of T-2 toxin in chondrocytes and cartilages. HSP47 plays a role in the degenerative changes of KBD and associated with T-2 toxin-induced decreased type II collagen expression, further promoting matrix degradation.

Authors+Show Affiliations

School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China.School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases in National Health Commission of PR of China, Xi'an Jiaotong University, Xi'an, Shaanxi, China. chenjh.123@mail.xjtu.edu.cn. The Institute of Endemic Diseases, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China. chenjh.123@mail.xjtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32591934

Citation

Zhang, Meng, et al. "Decreased Expression of Heat Shock Protein 47 Is Associated With T-2 Toxin and Low Selenium-Induced Matrix Degradation in Cartilages of Kashin-Beck Disease." Biological Trace Element Research, 2020.
Zhang M, Wang M, Wang H, et al. Decreased Expression of Heat Shock Protein 47 Is Associated with T-2 Toxin and Low Selenium-Induced Matrix Degradation in Cartilages of Kashin-Beck Disease. Biol Trace Elem Res. 2020.
Zhang, M., Wang, M., Wang, H., Zhang, Y., Li, Z., Feng, Y., Liu, Y., Liu, Y., Liao, Y., Wang, W., Fang, Q., & Chen, J. (2020). Decreased Expression of Heat Shock Protein 47 Is Associated with T-2 Toxin and Low Selenium-Induced Matrix Degradation in Cartilages of Kashin-Beck Disease. Biological Trace Element Research. https://doi.org/10.1007/s12011-020-02237-1
Zhang M, et al. Decreased Expression of Heat Shock Protein 47 Is Associated With T-2 Toxin and Low Selenium-Induced Matrix Degradation in Cartilages of Kashin-Beck Disease. Biol Trace Elem Res. 2020 Jun 26; PubMed PMID: 32591934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Decreased Expression of Heat Shock Protein 47 Is Associated with T-2 Toxin and Low Selenium-Induced Matrix Degradation in Cartilages of Kashin-Beck Disease. AU - Zhang,Meng, AU - Wang,Mengying, AU - Wang,Hui, AU - Zhang,Ying, AU - Li,Zhengzheng, AU - Feng,Yiping, AU - Liu,Yinan, AU - Liu,Yue, AU - Liao,Yucheng, AU - Wang,Wenjun, AU - Fang,Qian, AU - Chen,Jinghong, Y1 - 2020/06/26/ PY - 2020/04/16/received PY - 2020/06/08/accepted PY - 2020/6/28/entrez KW - Heat shock protein 47 KW - Kashin-Beck disease KW - Matrix degradation KW - Selenium KW - T-2 toxin KW - Type II collagen JF - Biological trace element research JO - Biol Trace Elem Res N2 - Recent evidence suggests a role of type II collagen in Kashin-Beck disease (KBD) degeneration. We aimed to assess the abnormal expression of heat shock protein 47 (HSP47) which is associated with a decrease in type II collagen and an increase in cartilage degradation in KBD. Hand phalange cartilages were collected from KBD and healthy children. Rats were administered with T-2 toxin under the selenium (Se)-deficient diet. ATDC5 cells were seeded on bone matrix gelatin to construct engineered cartilaginous tissue. C28/I2 and ATDC5 cells and engineered tissue were exposed to different concentrations of T-2 toxin with or without Se. Cartilage degeneration was determined through histological evaluation. The distribution and expression of type II collagen and HSP47 were investigated through immunohistochemistry, western blotting, and real-time PCR. KBD cartilages showed increased chondronecrosis and extracellular matrix degradation in deep zone with decreased type II collagen and HSP47 expression. The low-Se + T-2 toxin animal group showed a significantly lower type II collagen expression along with decreased HSP47 expression. Decreased type II collagen and HSP47 in C28/I2 and ATDC5 cells induced by T-2 toxin showed a dose-dependent manner. Hyaline-like cartilage with zonal layers was developed in engineered cartilaginous tissues, with decreased type II collagen and HSP47 expression found in T-2 toxin-treated group. Se-supplementation partially antagonized the inhibitory effects of T-2 toxin in chondrocytes and cartilages. HSP47 plays a role in the degenerative changes of KBD and associated with T-2 toxin-induced decreased type II collagen expression, further promoting matrix degradation. SN - 1559-0720 UR - https://www.unboundmedicine.com/medline/citation/32591934/Decreased_Expression_of_Heat_Shock_Protein_47_Is_Associated_with_T-2_Toxin_and_Low_Selenium-Induced_Matrix_Degradation_in_Cartilages_of_Kashin-Beck_Disease L2 - https://dx.doi.org/10.1007/s12011-020-02237-1 DB - PRIME DP - Unbound Medicine ER -
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