Tags

Type your tag names separated by a space and hit enter

In vivo microstimulation with cathodic and anodic asymmetric waveforms modulates spatiotemporal calcium dynamics in cortical neuropil and pyramidal neurons of male mice.
J Neurosci Res. 2020 Jun 26 [Online ahead of print]JN

Abstract

Electrical stimulation has been critical in the development of an understanding of brain function and disease. Despite its widespread use and obvious clinical potential, the mechanisms governing stimulation in the cortex remain largely unexplored in the context of pulse parameters. Modeling studies have suggested that modulation of stimulation pulse waveform may be able to control the probability of neuronal activation to selectively stimulate either cell bodies or passing fibers depending on the leading polarity. Thus, asymmetric waveforms with equal charge per phase (i.e., increasing the leading phase duration and proportionately decreasing the amplitude) may be able to activate a more spatially localized or distributed population of neurons if the leading phase is cathodic or anodic, respectively. Here, we use two-photon and mesoscale calcium imaging of GCaMP6s expressed in excitatory pyramidal neurons of male mice to investigate the role of pulse polarity and waveform asymmetry on the spatiotemporal properties of direct neuronal activation with 10-Hz electrical stimulation. We demonstrate that increasing cathodic asymmetry effectively reduces neuronal activation and results in a more spatially localized subpopulation of activated neurons without sacrificing the density of activated neurons around the electrode. Conversely, increasing anodic asymmetry increases the spatial spread of activation and highly resembles spatiotemporal calcium activity induced by conventional symmetric cathodic stimulation. These results suggest that stimulation polarity and asymmetry can be used to modulate the spatiotemporal dynamics of neuronal activity thus increasing the effective parameter space of electrical stimulation to restore sensation and study circuit dynamics.

Authors+Show Affiliations

Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA. Center for the Neural Basis of Cognition, University of Pittsburgh, Carnegie Mellon University, Pittsburgh, PA, USA.Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI, USA. Department of Neurological Surgery, University of Wisconsin Madison, Madison, WI, USA. Wisconsin Institute for Translational Neuroengineering (WITNe), Madison, WI, USA.Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA. Center for the Neural Basis of Cognition, University of Pittsburgh, Carnegie Mellon University, Pittsburgh, PA, USA. Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA, USA. McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA. NeuroTech Center, University of Pittsburgh Brain Institute, Pittsburgh, PA, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32592267

Citation

Stieger, Kevin C., et al. "In Vivo Microstimulation With Cathodic and Anodic Asymmetric Waveforms Modulates Spatiotemporal Calcium Dynamics in Cortical Neuropil and Pyramidal Neurons of Male Mice." Journal of Neuroscience Research, 2020.
Stieger KC, Eles JR, Ludwig KA, et al. In vivo microstimulation with cathodic and anodic asymmetric waveforms modulates spatiotemporal calcium dynamics in cortical neuropil and pyramidal neurons of male mice. J Neurosci Res. 2020.
Stieger, K. C., Eles, J. R., Ludwig, K. A., & Kozai, T. D. Y. (2020). In vivo microstimulation with cathodic and anodic asymmetric waveforms modulates spatiotemporal calcium dynamics in cortical neuropil and pyramidal neurons of male mice. Journal of Neuroscience Research. https://doi.org/10.1002/jnr.24676
Stieger KC, et al. In Vivo Microstimulation With Cathodic and Anodic Asymmetric Waveforms Modulates Spatiotemporal Calcium Dynamics in Cortical Neuropil and Pyramidal Neurons of Male Mice. J Neurosci Res. 2020 Jun 26; PubMed PMID: 32592267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo microstimulation with cathodic and anodic asymmetric waveforms modulates spatiotemporal calcium dynamics in cortical neuropil and pyramidal neurons of male mice. AU - Stieger,Kevin C, AU - Eles,James R, AU - Ludwig,Kip A, AU - Kozai,Takashi D Y, Y1 - 2020/06/26/ PY - 2019/12/18/received PY - 2020/05/26/revised PY - 2020/05/27/accepted PY - 2020/6/28/entrez KW - GCaMP6 KW - calcium imaging KW - electrical stimulation KW - neuromodulation KW - two-photon JF - Journal of neuroscience research JO - J. Neurosci. Res. N2 - Electrical stimulation has been critical in the development of an understanding of brain function and disease. Despite its widespread use and obvious clinical potential, the mechanisms governing stimulation in the cortex remain largely unexplored in the context of pulse parameters. Modeling studies have suggested that modulation of stimulation pulse waveform may be able to control the probability of neuronal activation to selectively stimulate either cell bodies or passing fibers depending on the leading polarity. Thus, asymmetric waveforms with equal charge per phase (i.e., increasing the leading phase duration and proportionately decreasing the amplitude) may be able to activate a more spatially localized or distributed population of neurons if the leading phase is cathodic or anodic, respectively. Here, we use two-photon and mesoscale calcium imaging of GCaMP6s expressed in excitatory pyramidal neurons of male mice to investigate the role of pulse polarity and waveform asymmetry on the spatiotemporal properties of direct neuronal activation with 10-Hz electrical stimulation. We demonstrate that increasing cathodic asymmetry effectively reduces neuronal activation and results in a more spatially localized subpopulation of activated neurons without sacrificing the density of activated neurons around the electrode. Conversely, increasing anodic asymmetry increases the spatial spread of activation and highly resembles spatiotemporal calcium activity induced by conventional symmetric cathodic stimulation. These results suggest that stimulation polarity and asymmetry can be used to modulate the spatiotemporal dynamics of neuronal activity thus increasing the effective parameter space of electrical stimulation to restore sensation and study circuit dynamics. SN - 1097-4547 UR - https://www.unboundmedicine.com/medline/citation/32592267/In_vivo_microstimulation_with_cathodic_and_anodic_asymmetric_waveforms_modulates_spatiotemporal_calcium_dynamics_in_cortical_neuropil_and_pyramidal_neurons_of_male_mice L2 - https://doi.org/10.1002/jnr.24676 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.