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Assessing CD 10 Expression Level and Its Prognostic Impact in Egyptian Patients with Urothelial Carcinoma.
Asian Pac J Cancer Prev. 2020 Jun 01; 21(6):1573-1583.AP

Abstract

BACKGROUND AND AIM

CD10 is expressed in urothelial carcinoma cells and cancer associated fibroblasts (CAF). In the current study, CD10 immunohistochemical staining (IHC) and CD10 mRNA expression in urothelial carcinoma of bladder (UCB) were assessed, and its relationship with tumor progression and prognosis was investigated.

PATIENTS AND METHODS

In this study, 106 formalin fixed paraffin-embedded (FFPE) tissue of UCB, obtained through radical cystectomy specimen, and 10 matched normal tissue samples were included.CD10 expression was evaluated by immunohistochemistry and real time PCR techniques.

RESULTS

CD10 expression in tumor cells and associated stromal fibroblasts was significantly associated with high tumor grade and advanced stage. Significant correlation was found between CD10 tumor expression and lymphovascular invasion (LVI) (P<0.001) as well as perineural invasion (PNI). CD10 expression in stromal fibroblasts was significantly associated with squamous differentiation of tumor cells, lymph node metastasis (LNM), and tumor necrosis. Positive CD10 expression in both tumor cells and associated stromal fibroblasts was associated with shorter OS . CD10 mRNA was overexpressed in tumors in comparison with the matched normal tissues. CD10 mRNA was significantly higher in invasive tumor, advanced stage tumor, and high grade tumor. There was significant correlation between CD10 mRNA tumor expression and LVI, PNI, and tumor recurrence.

CONCLUSION

Increased expression of CD10 in the tumor and CAF was strongly correlated with tumor progression, invasion, metastasis, shorter OS, and RFS in urothelial carcinoma patients. CD10 mRNA showed significantly higher expression in tumor tissue than in matched normal tissue. CD10 mRNA was associated with depth of invasion, TNM stage, tumor grade, vascular tumor invasion, and tumor recurrence.

Authors+Show Affiliations

Department of Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.Department of Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.Department of Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.Sohag university medical adminstartion,Sohag, Egypt.Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Mansoura University Mansoura, Egypt.Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt. Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH , USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32592351

Citation

Hussien, Marwa T., et al. "Assessing CD 10 Expression Level and Its Prognostic Impact in Egyptian Patients With Urothelial Carcinoma." Asian Pacific Journal of Cancer Prevention : APJCP, vol. 21, no. 6, 2020, pp. 1573-1583.
Hussien MT, Helmy E, Elsaba TM, et al. Assessing CD 10 Expression Level and Its Prognostic Impact in Egyptian Patients with Urothelial Carcinoma. Asian Pac J Cancer Prev. 2020;21(6):1573-1583.
Hussien, M. T., Helmy, E., Elsaba, T. M., Elkady, A., Alrefai, H., & Hetta, H. F. (2020). Assessing CD 10 Expression Level and Its Prognostic Impact in Egyptian Patients with Urothelial Carcinoma. Asian Pacific Journal of Cancer Prevention : APJCP, 21(6), 1573-1583. https://doi.org/10.31557/APJCP.2020.21.6.1573
Hussien MT, et al. Assessing CD 10 Expression Level and Its Prognostic Impact in Egyptian Patients With Urothelial Carcinoma. Asian Pac J Cancer Prev. 2020 Jun 1;21(6):1573-1583. PubMed PMID: 32592351.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessing CD 10 Expression Level and Its Prognostic Impact in Egyptian Patients with Urothelial Carcinoma. AU - Hussien,Marwa T, AU - Helmy,Eatemad, AU - Elsaba,Tarek M, AU - Elkady,Azza, AU - Alrefai,Hani, AU - Hetta,Helal F, Y1 - 2020/06/01/ PY - 2019/01/01/received PY - 2020/6/28/entrez PY - 2020/6/28/pubmed PY - 2020/6/28/medline KW - CD10 expression KW - Metastasis KW - Recurrence KW - Urothelial KW - stage SP - 1573 EP - 1583 JF - Asian Pacific journal of cancer prevention : APJCP JO - Asian Pac. J. Cancer Prev. VL - 21 IS - 6 N2 - BACKGROUND AND AIM: CD10 is expressed in urothelial carcinoma cells and cancer associated fibroblasts (CAF). In the current study, CD10 immunohistochemical staining (IHC) and CD10 mRNA expression in urothelial carcinoma of bladder (UCB) were assessed, and its relationship with tumor progression and prognosis was investigated. PATIENTS AND METHODS: In this study, 106 formalin fixed paraffin-embedded (FFPE) tissue of UCB, obtained through radical cystectomy specimen, and 10 matched normal tissue samples were included.CD10 expression was evaluated by immunohistochemistry and real time PCR techniques. RESULTS: CD10 expression in tumor cells and associated stromal fibroblasts was significantly associated with high tumor grade and advanced stage. Significant correlation was found between CD10 tumor expression and lymphovascular invasion (LVI) (P<0.001) as well as perineural invasion (PNI). CD10 expression in stromal fibroblasts was significantly associated with squamous differentiation of tumor cells, lymph node metastasis (LNM), and tumor necrosis. Positive CD10 expression in both tumor cells and associated stromal fibroblasts was associated with shorter OS . CD10 mRNA was overexpressed in tumors in comparison with the matched normal tissues. CD10 mRNA was significantly higher in invasive tumor, advanced stage tumor, and high grade tumor. There was significant correlation between CD10 mRNA tumor expression and LVI, PNI, and tumor recurrence. CONCLUSION: Increased expression of CD10 in the tumor and CAF was strongly correlated with tumor progression, invasion, metastasis, shorter OS, and RFS in urothelial carcinoma patients. CD10 mRNA showed significantly higher expression in tumor tissue than in matched normal tissue. CD10 mRNA was associated with depth of invasion, TNM stage, tumor grade, vascular tumor invasion, and tumor recurrence. SN - 2476-762X UR - https://www.unboundmedicine.com/medline/citation/32592351/Assessing_CD_10_Expression_Level_and_Its_Prognostic_Impact_in_Egyptian_Patients_with_Urothelial_Carcinoma L2 - http://journal.waocp.org/?sid=Entrez:PubMed&amp;id=pmid:32592351&amp;key=2020.21.6.1573 DB - PRIME DP - Unbound Medicine ER -
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