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Effects of prescription antibiotics on soil- and root-associated microbiomes and resistomes in an agricultural context.
J Hazard Mater. 2020 Jun 17; 400:123208.JH

Abstract

The use of treated wastewater for crop irrigation is rapidly increasing to respond to the ever-growing demands for water and food resources. However, this practice may contribute to the spread of antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARGs) in agricultural settings. To evaluate this potential risk, we analyzed microbiomes and resistomes of soil and Lactuca sativa L. (lettuce) root samples from pots irrigated with tap water spiked with 0, 20, or 100 μg L-1 of a mixture of three antibiotics (Trimethoprim, Ofloxacin, Sulfamethoxazole). The presence of antibiotics induced changes in bacterial populations, particularly in soil, as revealed by 16S rDNA sequence analysis. Parallel shotgun sequencing identified a total of 56 different ARGs conferring resistance against 14 antibiotic families. Antibiotic -treated samples showed increased loads of ARGs implicated in mutidrug resistance or in both direct and indirect acquired resistance. These changes correlated with the prevalence of Xantomonadales species in the root microbiomes. We interpret these data as indicating different strategies of soil and root microbiomes to cope with the presence of antibiotics, and as a warning that their presence may increase the loads of ARBs and ARGs in edible plant parts, therefore constituting a potential risk for human consumers.

Authors+Show Affiliations

Institute of Environmental Assessment and Water Research (IDAEA), Spanish National Research Council, Barcelona, Spain.Agricultural Research Institute, Ministry of Agriculture, Rural Development and Environment, P.O. Box 22016, 1516, Nicosia, Cyprus.Civil and Environmental Engineering Department and Nireas, International Water Research Center, University of Cyprus, P.O. Box 20538, 1678, Nicosia, Cyprus.Institute of Environmental Assessment and Water Research (IDAEA), Spanish National Research Council, Barcelona, Spain.Institute of Environmental Assessment and Water Research (IDAEA), Spanish National Research Council, Barcelona, Spain.Institute of Environmental Assessment and Water Research (IDAEA), Spanish National Research Council, Barcelona, Spain. Electronic address: bpcbmc@cid.csic.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32593021

Citation

Cerqueira, Francisco, et al. "Effects of Prescription Antibiotics On Soil- and Root-associated Microbiomes and Resistomes in an Agricultural Context." Journal of Hazardous Materials, vol. 400, 2020, p. 123208.
Cerqueira F, Christou A, Fatta-Kassinos D, et al. Effects of prescription antibiotics on soil- and root-associated microbiomes and resistomes in an agricultural context. J Hazard Mater. 2020;400:123208.
Cerqueira, F., Christou, A., Fatta-Kassinos, D., Vila-Costa, M., Bayona, J. M., & Piña, B. (2020). Effects of prescription antibiotics on soil- and root-associated microbiomes and resistomes in an agricultural context. Journal of Hazardous Materials, 400, 123208. https://doi.org/10.1016/j.jhazmat.2020.123208
Cerqueira F, et al. Effects of Prescription Antibiotics On Soil- and Root-associated Microbiomes and Resistomes in an Agricultural Context. J Hazard Mater. 2020 Jun 17;400:123208. PubMed PMID: 32593021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of prescription antibiotics on soil- and root-associated microbiomes and resistomes in an agricultural context. AU - Cerqueira,Francisco, AU - Christou,Anastasis, AU - Fatta-Kassinos,Despo, AU - Vila-Costa,Maria, AU - Bayona,Josep Maria, AU - Piña,Benjamin, Y1 - 2020/06/17/ PY - 2020/02/07/received PY - 2020/05/22/revised PY - 2020/06/11/accepted PY - 2020/6/28/pubmed PY - 2020/6/28/medline PY - 2020/6/28/entrez KW - Antibiotic resistance KW - Lactuca sativa L. KW - Metagenomics KW - Microbiome KW - Resistome KW - Root endosphere KW - Soil SP - 123208 EP - 123208 JF - Journal of hazardous materials JO - J. Hazard. Mater. VL - 400 N2 - The use of treated wastewater for crop irrigation is rapidly increasing to respond to the ever-growing demands for water and food resources. However, this practice may contribute to the spread of antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARGs) in agricultural settings. To evaluate this potential risk, we analyzed microbiomes and resistomes of soil and Lactuca sativa L. (lettuce) root samples from pots irrigated with tap water spiked with 0, 20, or 100 μg L-1 of a mixture of three antibiotics (Trimethoprim, Ofloxacin, Sulfamethoxazole). The presence of antibiotics induced changes in bacterial populations, particularly in soil, as revealed by 16S rDNA sequence analysis. Parallel shotgun sequencing identified a total of 56 different ARGs conferring resistance against 14 antibiotic families. Antibiotic -treated samples showed increased loads of ARGs implicated in mutidrug resistance or in both direct and indirect acquired resistance. These changes correlated with the prevalence of Xantomonadales species in the root microbiomes. We interpret these data as indicating different strategies of soil and root microbiomes to cope with the presence of antibiotics, and as a warning that their presence may increase the loads of ARBs and ARGs in edible plant parts, therefore constituting a potential risk for human consumers. SN - 1873-3336 UR - https://www.unboundmedicine.com/medline/citation/32593021/Effects_of_prescription_antibiotics_on_soil-_and_root-associated_microbiomes_and_resistomes_in_an_agricultural_context L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3894(20)31197-3 DB - PRIME DP - Unbound Medicine ER -
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