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Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results.
Clin Microbiol Infect. 2020 Jun 25 [Online ahead of print]CM

Abstract

BACKGROUND

To face the current COVID-19 pandemic, diagnostic tools are essential. It is recommended to use real-time RT-PCR for RNA viruses in order (a) to perform a rapid and accurate diagnostic, (b) to guide patient care and management and (c) to guide epidemiological strategies. Further studies are warranted to define the role of serological diagnosis and a possible correlation between serological response and prognosis.

OBJECTIVES

The aim was to guide clinical microbiologists in the use of these diagnostic tests and clinicians in the interpretation of their results.

SOURCES

A search of literature was performed through PubMed and Google Scholar using the keywords SARS-CoV-2, SARS-CoV-2 molecular diagnosis, SARS-CoV-2 immune response, SARS-CoV-2 serology/antibody testing, coronavirus diagnosis.

CONTENT

The present review discusses performances, limitations and use of current and future diagnostic tests for SARS-CoV-2.

IMPLICATIONS

Real-time RT-PCR remains the reference method for diagnosis of SARS-CoV-2 infection. On the other hand, notwithstanding its varying sensitivity according to the time of infection, serology represents a valid asset (a) to try to solve possible discrepancies between a highly suggestive clinical and radiological presentation and negative RT-PCR, (b) to solve discrepancies between different PCR assays and (c) for epidemiological purposes.

Authors+Show Affiliations

Institut de Microbiologie, Département de médecine de laboratoire et pathologie (DMLP), Centre Hospitalier Universitaire Vaudois and Université de Lausanne, Lausanne, Switzerland.Institut de Microbiologie, Département de médecine de laboratoire et pathologie (DMLP), Centre Hospitalier Universitaire Vaudois and Université de Lausanne, Lausanne, Switzerland.Institut de Microbiologie, Département de médecine de laboratoire et pathologie (DMLP), Centre Hospitalier Universitaire Vaudois and Université de Lausanne, Lausanne, Switzerland.Institut de Microbiologie, Département de médecine de laboratoire et pathologie (DMLP), Centre Hospitalier Universitaire Vaudois and Université de Lausanne, Lausanne, Switzerland.Institut de Microbiologie, Département de médecine de laboratoire et pathologie (DMLP), Centre Hospitalier Universitaire Vaudois and Université de Lausanne, Lausanne, Switzerland; Service de Maladies Infectieuses, Département de médecine, Centre Hospitalier Universitaire Vaudois and Université de Lausanne, Lausanne, Switzerland.Institut de Microbiologie, Département de médecine de laboratoire et pathologie (DMLP), Centre Hospitalier Universitaire Vaudois and Université de Lausanne, Lausanne, Switzerland.Institut de Microbiologie, Département de médecine de laboratoire et pathologie (DMLP), Centre Hospitalier Universitaire Vaudois and Université de Lausanne, Lausanne, Switzerland; Service de Maladies Infectieuses, Département de médecine, Centre Hospitalier Universitaire Vaudois and Université de Lausanne, Lausanne, Switzerland. Electronic address: gilbert.greub@chuv.ch.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

32593741

Citation

Caruana, G, et al. "Diagnostic Strategies for SARS-CoV-2 Infection and Interpretation of Microbiological Results." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 2020.
Caruana G, Croxatto A, Coste AT, et al. Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results. Clin Microbiol Infect. 2020.
Caruana, G., Croxatto, A., Coste, A. T., Opota, O., Lamoth, F., Jaton, K., & Greub, G. (2020). Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases. https://doi.org/10.1016/j.cmi.2020.06.019
Caruana G, et al. Diagnostic Strategies for SARS-CoV-2 Infection and Interpretation of Microbiological Results. Clin Microbiol Infect. 2020 Jun 25; PubMed PMID: 32593741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results. AU - Caruana,G, AU - Croxatto,A, AU - Coste,A T, AU - Opota,O, AU - Lamoth,F, AU - Jaton,K, AU - Greub,G, Y1 - 2020/06/25/ PY - 2020/04/17/received PY - 2020/06/15/revised PY - 2020/06/20/accepted PY - 2020/7/1/pubmed PY - 2020/7/1/medline PY - 2020/6/29/entrez KW - COVID-19 KW - Coronavirus KW - Molecular diagnostic testing KW - Reverse transcriptase polymerase chain reaction KW - SARS-COV-2 KW - Serology JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JO - Clin. Microbiol. Infect. N2 - BACKGROUND: To face the current COVID-19 pandemic, diagnostic tools are essential. It is recommended to use real-time RT-PCR for RNA viruses in order (a) to perform a rapid and accurate diagnostic, (b) to guide patient care and management and (c) to guide epidemiological strategies. Further studies are warranted to define the role of serological diagnosis and a possible correlation between serological response and prognosis. OBJECTIVES: The aim was to guide clinical microbiologists in the use of these diagnostic tests and clinicians in the interpretation of their results. SOURCES: A search of literature was performed through PubMed and Google Scholar using the keywords SARS-CoV-2, SARS-CoV-2 molecular diagnosis, SARS-CoV-2 immune response, SARS-CoV-2 serology/antibody testing, coronavirus diagnosis. CONTENT: The present review discusses performances, limitations and use of current and future diagnostic tests for SARS-CoV-2. IMPLICATIONS: Real-time RT-PCR remains the reference method for diagnosis of SARS-CoV-2 infection. On the other hand, notwithstanding its varying sensitivity according to the time of infection, serology represents a valid asset (a) to try to solve possible discrepancies between a highly suggestive clinical and radiological presentation and negative RT-PCR, (b) to solve discrepancies between different PCR assays and (c) for epidemiological purposes. SN - 1469-0691 UR - https://www.unboundmedicine.com/medline/citation/32593741/Diagnostic_strategies_for_SARS_CoV_2_infection_and_interpretation_of_microbiological_results_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(20)30363-3 DB - PRIME DP - Unbound Medicine ER -
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