Tags

Type your tag names separated by a space and hit enter

Treg and TH17 link to immune response in individuals with peri-implantitis: a preliminary report.
Clin Oral Investig. 2020 Jun 27 [Online ahead of print]CO

Abstract

BACKGROUND AND OBJECTIVES

Treg and TH17 cells influence the inflammatory process in periodontal diseases and could also play in a similar pattern, an essential role in immune-inflammatory mechanisms involved in the destruction of the peri-implant tissues, peri-implantitis. Therefore, this study evaluated the levels of RORγT and FOXP3 gene expression in subjects with peri-implantitis and healthy peri-implant tissues.

METHODS

A total of 35 subjects with implant-supported restorations in both diseased and healthy clinical conditions (n = 15 healthy; n = 20 peri-implantitis) were included in this study. Peri-implantitis was defined as probing depth > 5 mm, bleeding on probing and/or suppuration, and peri-implant bone loss >4 mm. Peri-implant tissue biopsies were collected for analysis of the mRNA, RORγT, and FOXP3 expression levels. The samples were submitted to total RNA extraction, treatment with DNAse, and cDNA synthesis. Subsequently, real-time PCR reaction was performed to evaluate the levels of RORγT and FOXP3 gene expression to the reference gene. These were analyzed by the non-parametric Mann-Whitney method with a level of significance of 5%.

RESULTS

Higher gene expression levels of the transcription factors RORγT and FOXP3 were detected in the tissues affected by peri-implantitis when compared with healthy tissues (p < 0.05).

CONCLUSIONS

The present study demonstrated the possible existence of a hybrid TH17-Treg profile, based on the gene expression of transcription factors inducing differentiation of these cells. Further studies must be designed to gain a better understanding of the immunological mechanisms involved in the pathogenesis of peri-implantitis.

CLINICAL RELEVANCE

The levels of RORγT and FOXP3 transcription factors that were linked to cells with the FOXP3+RORγT+ phenotype could be used as a predictor of peri-implantitis progression.

Authors+Show Affiliations

Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos (UnG), Guarulhos, SP, Brazil.Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos (UnG), Guarulhos, SP, Brazil.Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos (UnG), Guarulhos, SP, Brazil.Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos (UnG), Guarulhos, SP, Brazil.Department of Aging and Elderly Science, Sao Judas Tadeu University, Sao Paulo, Brazil.Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos (UnG), Guarulhos, SP, Brazil. jshibli@ung.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32594309

Citation

Giro, Gabriela, et al. "Treg and TH17 Link to Immune Response in Individuals With Peri-implantitis: a Preliminary Report." Clinical Oral Investigations, 2020.
Giro G, Tebar A, Franco L, et al. Treg and TH17 link to immune response in individuals with peri-implantitis: a preliminary report. Clin Oral Investig. 2020.
Giro, G., Tebar, A., Franco, L., Racy, D., Bastos, M. F., & Shibli, J. A. (2020). Treg and TH17 link to immune response in individuals with peri-implantitis: a preliminary report. Clinical Oral Investigations. https://doi.org/10.1007/s00784-020-03435-w
Giro G, et al. Treg and TH17 Link to Immune Response in Individuals With Peri-implantitis: a Preliminary Report. Clin Oral Investig. 2020 Jun 27; PubMed PMID: 32594309.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Treg and TH17 link to immune response in individuals with peri-implantitis: a preliminary report. AU - Giro,Gabriela, AU - Tebar,Andressa, AU - Franco,Leonardo, AU - Racy,Danilo, AU - Bastos,Marta Ferreira, AU - Shibli,Jamil Awad, Y1 - 2020/06/27/ PY - 2020/03/23/received PY - 2020/06/24/accepted PY - 2020/6/29/entrez KW - Bone loss KW - Dental implants KW - Immunology KW - Peri-implantitis KW - Treg and TH17 cells JF - Clinical oral investigations JO - Clin Oral Investig N2 - BACKGROUND AND OBJECTIVES: Treg and TH17 cells influence the inflammatory process in periodontal diseases and could also play in a similar pattern, an essential role in immune-inflammatory mechanisms involved in the destruction of the peri-implant tissues, peri-implantitis. Therefore, this study evaluated the levels of RORγT and FOXP3 gene expression in subjects with peri-implantitis and healthy peri-implant tissues. METHODS: A total of 35 subjects with implant-supported restorations in both diseased and healthy clinical conditions (n = 15 healthy; n = 20 peri-implantitis) were included in this study. Peri-implantitis was defined as probing depth > 5 mm, bleeding on probing and/or suppuration, and peri-implant bone loss >4 mm. Peri-implant tissue biopsies were collected for analysis of the mRNA, RORγT, and FOXP3 expression levels. The samples were submitted to total RNA extraction, treatment with DNAse, and cDNA synthesis. Subsequently, real-time PCR reaction was performed to evaluate the levels of RORγT and FOXP3 gene expression to the reference gene. These were analyzed by the non-parametric Mann-Whitney method with a level of significance of 5%. RESULTS: Higher gene expression levels of the transcription factors RORγT and FOXP3 were detected in the tissues affected by peri-implantitis when compared with healthy tissues (p < 0.05). CONCLUSIONS: The present study demonstrated the possible existence of a hybrid TH17-Treg profile, based on the gene expression of transcription factors inducing differentiation of these cells. Further studies must be designed to gain a better understanding of the immunological mechanisms involved in the pathogenesis of peri-implantitis. CLINICAL RELEVANCE: The levels of RORγT and FOXP3 transcription factors that were linked to cells with the FOXP3+RORγT+ phenotype could be used as a predictor of peri-implantitis progression. SN - 1436-3771 UR - https://www.unboundmedicine.com/medline/citation/32594309/Treg_and_TH17_link_to_immune_response_in_individuals_with_peri-implantitis:_a_preliminary_report L2 - https://dx.doi.org/10.1007/s00784-020-03435-w DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.