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Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells.
Front Plant Sci. 2020; 11:816.FP

Abstract

Foliar application of dsRNA to elicit an RNA interference (RNAi) response is currently under consideration as a crop protection strategy. To access the RNAi machinery of a plant, foliarly applied dsRNAs must traverse the plant cuticle, avoid nuclease degradation, and penetrate the cell wall and plasma membrane. Application methods and co-formulants have been identified by Bayer Crop Science researchers and others that can help bypass barriers to dsRNA uptake in plants leading to an RNAi response in greenhouse grown, young plants and cell cultures. However, these advances in dsRNA delivery have yet to yield systemic RNAi silencing of an endogenous gene target required for product concepts such as weed control. Systemic RNAi silencing in plants has only been observed with the GFP transgene in Nicotiana benthamiana. Because biologically meaningful whole plant RNAi has not been observed for endogenous gene products in N. benthamiana or in other plant species tested, under growing conditions including field production, the regulatory risk assessment of foliarly applied dsRNA-based products should not consider exposure scenarios that include systemic response to small RNAs in treated plants.

Authors+Show Affiliations

Bayer AG, Woodland, CA, United States.Bayer AG, Woodland, CA, United States.Bayer AG, Woodland, CA, United States.Bayer AG, Woodland, CA, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32595687

Citation

Bennett, Michael, et al. "Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells." Frontiers in Plant Science, vol. 11, 2020, p. 816.
Bennett M, Deikman J, Hendrix B, et al. Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells. Front Plant Sci. 2020;11:816.
Bennett, M., Deikman, J., Hendrix, B., & Iandolino, A. (2020). Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells. Frontiers in Plant Science, 11, 816. https://doi.org/10.3389/fpls.2020.00816
Bennett M, et al. Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells. Front Plant Sci. 2020;11:816. PubMed PMID: 32595687.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells. AU - Bennett,Michael, AU - Deikman,Jill, AU - Hendrix,Bill, AU - Iandolino,Alberto, Y1 - 2020/06/12/ PY - 2020/01/31/received PY - 2020/05/20/accepted PY - 2020/6/30/entrez PY - 2020/7/1/pubmed PY - 2020/7/1/medline KW - RNAi KW - barriers in plants KW - dsRNA KW - local silencing KW - systemic silencing SP - 816 EP - 816 JF - Frontiers in plant science JO - Front Plant Sci VL - 11 N2 - Foliar application of dsRNA to elicit an RNA interference (RNAi) response is currently under consideration as a crop protection strategy. To access the RNAi machinery of a plant, foliarly applied dsRNAs must traverse the plant cuticle, avoid nuclease degradation, and penetrate the cell wall and plasma membrane. Application methods and co-formulants have been identified by Bayer Crop Science researchers and others that can help bypass barriers to dsRNA uptake in plants leading to an RNAi response in greenhouse grown, young plants and cell cultures. However, these advances in dsRNA delivery have yet to yield systemic RNAi silencing of an endogenous gene target required for product concepts such as weed control. Systemic RNAi silencing in plants has only been observed with the GFP transgene in Nicotiana benthamiana. Because biologically meaningful whole plant RNAi has not been observed for endogenous gene products in N. benthamiana or in other plant species tested, under growing conditions including field production, the regulatory risk assessment of foliarly applied dsRNA-based products should not consider exposure scenarios that include systemic response to small RNAs in treated plants. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/32595687/Barriers_to_Efficient_Foliar_Uptake_of_dsRNA_and_Molecular_Barriers_to_dsRNA_Activity_in_Plant_Cells L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32595687/ DB - PRIME DP - Unbound Medicine ER -
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