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Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus.
Endocr Regul. 2020 Apr 01; 54(2):77-84.ER

Abstract

OBJECTIVE

The goal of this study was to reveal the impact of four types of atypical antipsychotics including amisulpride (AMI), olanzapine (OLA), quetiapine (QUE), and aripiprazole (ARI), with different receptor-affinity profile and dissociation constant, on the activity of hypothalamic supraoptic nucleus (SON) vasopressinergic and oxytocinergic neurons.

METHODS

Male Sprague Dawley rats received a single injection of vehicle (VEH) (0.1 ml/100g), AMI (20 mg/kg), OLA (5 mg/kg), QUE (15 mg/kg/) or ARI (10 mg/kg). Ninety min after treatment, the animals were fixed by transcardial perfusion, the brains removed, and cryocut into serial coronal sections of 35 µm thickness. The sections were processed for c-Fos staining using an avidin-biotin-peroxidase complex and visualized by nickel intensified diaminobenzidine to reach black end product. Afterwards, the sections were exposed to vasopressin (AVP) and oxytocin (OXY) antibodies and the reaction product visualized by biotin-labeled fluorescent Alexa Fluor 568 dye. The data were evaluated from c-Fos and AVP or OXY merged sections.

RESULTS

The present study shows that all four antipsychotics applied induced c-Fos expression in the SON. With respect to the stimulation efficacy of the individual antipsychotics, estimated based on the quantity of c-Fos-labeled AVP and OXY neurons, could be a preferential action assigned to QUE over moderate effect of ARI and lower effect to OLA and reduced effect of AMI (VEH < AMI < OLA < ARI < QUE).

CONCLUSION

The present data for the first time provide an insight into the quantitative pattern of brain activity within the clusters of SON AVP and OXY cells in response to different atypical antipsychotics single treatment.

Authors+Show Affiliations

Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovak Republic.Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovak Republic.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32597151

Citation

Kiss, Alexander, and Jana Osacka. "Effect of Amisulpride, Olanzapine, Quetiapine, and Aripiprazole Single Administration On c-Fos Expression in Vasopressinergic and Oxytocinergic Neurons of the Rat Hypothalamic Supraoptic Nucleus." Endocrine Regulations, vol. 54, no. 2, 2020, pp. 77-84.
Kiss A, Osacka J. Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus. Endocr Regul. 2020;54(2):77-84.
Kiss, A., & Osacka, J. (2020). Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus. Endocrine Regulations, 54(2), 77-84. https://doi.org/10.2478/enr-2020-0010
Kiss A, Osacka J. Effect of Amisulpride, Olanzapine, Quetiapine, and Aripiprazole Single Administration On c-Fos Expression in Vasopressinergic and Oxytocinergic Neurons of the Rat Hypothalamic Supraoptic Nucleus. Endocr Regul. 2020 Apr 1;54(2):77-84. PubMed PMID: 32597151.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus. AU - Kiss,Alexander, AU - Osacka,Jana, PY - 2020/6/30/entrez KW - amisulpride KW - aripiprazole KW - c-Fos-immunohistochemistry KW - hypothalamic supraoptic nucleus KW - olanzapine KW - quetiapine KW - rat SP - 77 EP - 84 JF - Endocrine regulations JO - Endocr Regul VL - 54 IS - 2 N2 - OBJECTIVE: The goal of this study was to reveal the impact of four types of atypical antipsychotics including amisulpride (AMI), olanzapine (OLA), quetiapine (QUE), and aripiprazole (ARI), with different receptor-affinity profile and dissociation constant, on the activity of hypothalamic supraoptic nucleus (SON) vasopressinergic and oxytocinergic neurons. METHODS: Male Sprague Dawley rats received a single injection of vehicle (VEH) (0.1 ml/100g), AMI (20 mg/kg), OLA (5 mg/kg), QUE (15 mg/kg/) or ARI (10 mg/kg). Ninety min after treatment, the animals were fixed by transcardial perfusion, the brains removed, and cryocut into serial coronal sections of 35 µm thickness. The sections were processed for c-Fos staining using an avidin-biotin-peroxidase complex and visualized by nickel intensified diaminobenzidine to reach black end product. Afterwards, the sections were exposed to vasopressin (AVP) and oxytocin (OXY) antibodies and the reaction product visualized by biotin-labeled fluorescent Alexa Fluor 568 dye. The data were evaluated from c-Fos and AVP or OXY merged sections. RESULTS: The present study shows that all four antipsychotics applied induced c-Fos expression in the SON. With respect to the stimulation efficacy of the individual antipsychotics, estimated based on the quantity of c-Fos-labeled AVP and OXY neurons, could be a preferential action assigned to QUE over moderate effect of ARI and lower effect to OLA and reduced effect of AMI (VEH < AMI < OLA < ARI < QUE). CONCLUSION: The present data for the first time provide an insight into the quantitative pattern of brain activity within the clusters of SON AVP and OXY cells in response to different atypical antipsychotics single treatment. SN - 1336-0329 UR - https://www.unboundmedicine.com/medline/citation/32597151/Effect_of_amisulpride,_olanzapine,_quetiapine,_and_aripiprazole_single_administration_on_c-Fos_expression_in_vasopressinergic_and_oxytocinergic_neurons_of_the_rat_hypothalamic_supraoptic_nucleus L2 - https://www.degruyter.com/doi/10.2478/enr-2020-0010 DB - PRIME DP - Unbound Medicine ER -
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