Tags

Type your tag names separated by a space and hit enter

NELL2 Function in Axon Development of Hippocampal Neurons.
Mol Cells. 2020 Jun 30; 43(6):581-589.MC

Abstract

Neurons have multiple dendrites and single axon. This neuronal polarity is gradually established during early processes of neuronal differentiation: generation of multiple neurites (stages 1-2); differentiation (stage 3) and maturation (stages 4-5) of an axon and dendrites. In this study, we demonstrated that the neuron-specific n-glycosylated protein NELL2 is important for neuronal polarization and axon growth using cultured rat embryonic hippocampal neurons. Endogenous NELL2 expression was gradually increased in parallel with the progression of developmental stages of hippocampal neurons, and overexpression of NELL2 stimulated neuronal polarization and axon growth. In line with these results, knockdown of NELL2 expression resulted in deterioration of neuronal development, including inhibition of neuronal development progression, decreased axon growth and increased axon branching. Inhibitor against extracellular signal-regulated kinase (ERK) dramatically inhibited NELL2-induced progression of neuronal development and axon growth. These results suggest that NELL2 is an important regulator for the morphological development for neuronal polarization and axon growth.

Authors+Show Affiliations

Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan 44610, Korea. Present address: Department of Pharmacology and Physiology, School of Medicine & Health Sciences, The George Washington University, Washington, D.C. 20037, USA. These authors contributed equally to this work.Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan 44610, Korea. These authors contributed equally to this work.Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan 44610, Korea. These authors contributed equally to this work.Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan 44610, Korea.Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan 44610, Korea.Center for Functional Connectomics, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea.Department of Oral Biochemistry, Dental and Life Science Institute, Pusan National University School of Dentistry, Yangsan 50612, Korea.Department of Oral Biochemistry, Dental and Life Science Institute, Pusan National University School of Dentistry, Yangsan 50612, Korea.Research Division and Brain Research Core Facilities of Korea Brain Research Institute, Daegu 41068, Korea.Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan 44610, Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32597395

Citation

Kim, Han Rae, et al. "NELL2 Function in Axon Development of Hippocampal Neurons." Molecules and Cells, vol. 43, no. 6, 2020, pp. 581-589.
Kim HR, Kim DH, An JY, et al. NELL2 Function in Axon Development of Hippocampal Neurons. Mol Cells. 2020;43(6):581-589.
Kim, H. R., Kim, D. H., An, J. Y., Kang, D., Park, J. W., Hwang, E. M., Seo, E. J., Jang, I. H., Ha, C. M., & Lee, B. J. (2020). NELL2 Function in Axon Development of Hippocampal Neurons. Molecules and Cells, 43(6), 581-589. https://doi.org/10.14348/molcells.2020.0032
Kim HR, et al. NELL2 Function in Axon Development of Hippocampal Neurons. Mol Cells. 2020 Jun 30;43(6):581-589. PubMed PMID: 32597395.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NELL2 Function in Axon Development of Hippocampal Neurons. AU - Kim,Han Rae, AU - Kim,Dong Hee, AU - An,Ji Young, AU - Kang,Dasol, AU - Park,Jeong Woo, AU - Hwang,Eun Mi, AU - Seo,Eun Jin, AU - Jang,Il Ho, AU - Ha,Chang Man, AU - Lee,Byung Ju, PY - 2020/01/29/received PY - 2020/05/06/revised PY - 2020/05/24/accepted PY - 2020/6/30/entrez PY - 2020/7/1/pubmed PY - 2020/7/1/medline KW - axon growth KW - extracellular signal-regulated kinase KW - hippocampal neurons KW - neural epidermal growth factor-like like protein 2 KW - neuronal polarity SP - 581 EP - 589 JF - Molecules and cells JO - Mol. Cells VL - 43 IS - 6 N2 - Neurons have multiple dendrites and single axon. This neuronal polarity is gradually established during early processes of neuronal differentiation: generation of multiple neurites (stages 1-2); differentiation (stage 3) and maturation (stages 4-5) of an axon and dendrites. In this study, we demonstrated that the neuron-specific n-glycosylated protein NELL2 is important for neuronal polarization and axon growth using cultured rat embryonic hippocampal neurons. Endogenous NELL2 expression was gradually increased in parallel with the progression of developmental stages of hippocampal neurons, and overexpression of NELL2 stimulated neuronal polarization and axon growth. In line with these results, knockdown of NELL2 expression resulted in deterioration of neuronal development, including inhibition of neuronal development progression, decreased axon growth and increased axon branching. Inhibitor against extracellular signal-regulated kinase (ERK) dramatically inhibited NELL2-induced progression of neuronal development and axon growth. These results suggest that NELL2 is an important regulator for the morphological development for neuronal polarization and axon growth. SN - 0219-1032 UR - https://www.unboundmedicine.com/medline/citation/32597395/NELL2_Function_in_Axon_Development_of_Hippocampal_Neurons L2 - https://dx.doi.org/10.14348/molcells.2020.0032 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.