Tags

Type your tag names separated by a space and hit enter

[Comparative characteristics of enzymatic hydrolysis methods for isolating toxic substances from blood and hair].
Sud Med Ekspert. 2020; 63(3):23-29.SM

Abstract

The aim of study was to develop methods for the hydrolysis with hyaluronidases for the isolation of drugs (phenobarbital, diphenhydramine hydrochloride and phenibut) from biological objects (blood, hair) and to compare their effectivenes with previously developed methods of enzymatic hydrolysis. The studies were carried out using model «blood - model drug substance (MDS)», a natural and artificially colored wool (hair) of laboratory animals - guinea pigs of white, red and black natural colors, which were daily given a solution of MDS and then were subjected to cosmetic effects - coloring. The isolation of MDS from the model complex «blood - MDS» and from wool was carried out using the developed methods of hydrolysis with proteolytic enzymes (papain, chymopsin and chymotrypsin) and hyaluronidase. Phenobarbital and diphenhydramine from hydrolysates were extracted by liquid-liquid extraction method, phenibut - by direct freezing extraction with acetonitrile. The analysis of the extracts was carried out by gas chromatography with mass selective detection. The results showed that the developed methods of enzymatic hydrolysis can be recommended for isolating substances of various properties (acidic, amphoteric and alkaline) from blood, natural and artificially colored wool (hair).

Authors+Show Affiliations

St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia.St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia.St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia.St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia.St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia.St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia.

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

32597607

Citation

Starovoitova, M K., et al. "[Comparative Characteristics of Enzymatic Hydrolysis Methods for Isolating Toxic Substances From Blood and Hair]." Sudebno-meditsinskaia Ekspertiza, vol. 63, no. 3, 2020, pp. 23-29.
Starovoitova MK, Minachenkova AS, Krys'Ko MV, et al. [Comparative characteristics of enzymatic hydrolysis methods for isolating toxic substances from blood and hair]. Sud Med Ekspert. 2020;63(3):23-29.
Starovoitova, M. K., Minachenkova, A. S., Krys'Ko, M. V., Slustovskaya, Y. V., Strelova, O. Y., & Kuklin, V. N. (2020). [Comparative characteristics of enzymatic hydrolysis methods for isolating toxic substances from blood and hair]. Sudebno-meditsinskaia Ekspertiza, 63(3), 23-29. https://doi.org/10.17116/sudmed20206303123
Starovoitova MK, et al. [Comparative Characteristics of Enzymatic Hydrolysis Methods for Isolating Toxic Substances From Blood and Hair]. Sud Med Ekspert. 2020;63(3):23-29. PubMed PMID: 32597607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparative characteristics of enzymatic hydrolysis methods for isolating toxic substances from blood and hair]. AU - Starovoitova,M K, AU - Minachenkova,A S, AU - Krys'Ko,M V, AU - Slustovskaya,Yu V, AU - Strelova,O Yu, AU - Kuklin,V N, PY - 2020/6/30/entrez KW - blood KW - chymopsin KW - chymotrypsin KW - diphenhydramine KW - enzymatic hydrolysis KW - hyaluronidases KW - natural and artificially colored hair KW - papain KW - phenibut KW - phenobarbital SP - 23 EP - 29 JF - Sudebno-meditsinskaia ekspertiza JO - Sud. Med. Ekspert. VL - 63 IS - 3 N2 - The aim of study was to develop methods for the hydrolysis with hyaluronidases for the isolation of drugs (phenobarbital, diphenhydramine hydrochloride and phenibut) from biological objects (blood, hair) and to compare their effectivenes with previously developed methods of enzymatic hydrolysis. The studies were carried out using model «blood - model drug substance (MDS)», a natural and artificially colored wool (hair) of laboratory animals - guinea pigs of white, red and black natural colors, which were daily given a solution of MDS and then were subjected to cosmetic effects - coloring. The isolation of MDS from the model complex «blood - MDS» and from wool was carried out using the developed methods of hydrolysis with proteolytic enzymes (papain, chymopsin and chymotrypsin) and hyaluronidase. Phenobarbital and diphenhydramine from hydrolysates were extracted by liquid-liquid extraction method, phenibut - by direct freezing extraction with acetonitrile. The analysis of the extracts was carried out by gas chromatography with mass selective detection. The results showed that the developed methods of enzymatic hydrolysis can be recommended for isolating substances of various properties (acidic, amphoteric and alkaline) from blood, natural and artificially colored wool (hair). SN - 0039-4521 UR - https://www.unboundmedicine.com/medline/citation/32597607/[Comparative_characteristics_of_enzymatic_hydrolysis_methods_for_isolating_toxic_substances_from_blood_and_hair] L2 - https://antibodies.cancer.gov/detail/CPTC-YWHAE-1 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.