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Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification.
Int J Infect Dis. 2020 Sep; 98:16-17.IJ

Abstract

Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel detection kit - the 2019 Novel Coronavirus Detection Kit (nCoV-DK) - halves the detection time by eliminating the steps of RNA extraction and purification. We evaluated the concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 specimens (74.6%) by direct PCR and in 55/71 specimens (77.5%) by nCoV-DK; the overall concordance rate was 94.4%: 95.2% for nasopharyngeal swab, 95.5% for saliva, and 85.7% for sputum. The nCoV-DK test effectively detects SARS-CoV-2 in all types of sample including saliva, while reducing the time required for detection, labor, and the risk of human error.

Authors+Show Affiliations

Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan.Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan.Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan.Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan.Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan.Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan.Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan.Department of Respiratory Medicine, Hokkaido University Faculty of Medicine, Sapporo, Japan.Department of Respiratory Medicine, Hokkaido University Faculty of Medicine, Sapporo, Japan.Department of Respiratory Medicine, Hokkaido University Faculty of Medicine, Sapporo, Japan.Department of Respiratory Medicine, Hokkaido University Faculty of Medicine, Sapporo, Japan.Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan.Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan.Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan; Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan. Electronic address: teshima@med.hokudai.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32599282

Citation

Fukumoto, Tatsuya, et al. "Efficacy of a Novel SARS-CoV-2 Detection Kit Without RNA Extraction and Purification." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 98, 2020, pp. 16-17.
Fukumoto T, Iwasaki S, Fujisawa S, et al. Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification. Int J Infect Dis. 2020;98:16-17.
Fukumoto, T., Iwasaki, S., Fujisawa, S., Hayasaka, K., Sato, K., Oguri, S., Taki, K., Nakakubo, S., Kamada, K., Yamashita, Y., Konno, S., Nishida, M., Sugita, J., & Teshima, T. (2020). Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 98, 16-17. https://doi.org/10.1016/j.ijid.2020.06.074
Fukumoto T, et al. Efficacy of a Novel SARS-CoV-2 Detection Kit Without RNA Extraction and Purification. Int J Infect Dis. 2020;98:16-17. PubMed PMID: 32599282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification. AU - Fukumoto,Tatsuya, AU - Iwasaki,Sumio, AU - Fujisawa,Shinichi, AU - Hayasaka,Kasumi, AU - Sato,Kaori, AU - Oguri,Satoshi, AU - Taki,Keisuke, AU - Nakakubo,Sho, AU - Kamada,Keisuke, AU - Yamashita,Yu, AU - Konno,Satoshi, AU - Nishida,Mutsumi, AU - Sugita,Junichi, AU - Teshima,Takanori, Y1 - 2020/06/26/ PY - 2020/06/03/received PY - 2020/06/21/revised PY - 2020/06/23/accepted PY - 2020/7/1/pubmed PY - 2020/9/20/medline PY - 2020/6/30/entrez KW - COVID-19 KW - PCR KW - SARS-CoV-2 KW - Saliva SP - 16 EP - 17 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int J Infect Dis VL - 98 N2 - Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel detection kit - the 2019 Novel Coronavirus Detection Kit (nCoV-DK) - halves the detection time by eliminating the steps of RNA extraction and purification. We evaluated the concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 specimens (74.6%) by direct PCR and in 55/71 specimens (77.5%) by nCoV-DK; the overall concordance rate was 94.4%: 95.2% for nasopharyngeal swab, 95.5% for saliva, and 85.7% for sputum. The nCoV-DK test effectively detects SARS-CoV-2 in all types of sample including saliva, while reducing the time required for detection, labor, and the risk of human error. SN - 1878-3511 UR - https://www.unboundmedicine.com/medline/citation/32599282/Efficacy_of_a_novel_SARS_CoV_2_detection_kit_without_RNA_extraction_and_purification_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1201-9712(20)30509-9 DB - PRIME DP - Unbound Medicine ER -