Tags

Type your tag names separated by a space and hit enter

Disruption of evolutionarily correlated tRNA elements impairs accurate decoding.
Proc Natl Acad Sci U S A. 2020 Jul 14; 117(28):16333-16338.PN

Abstract

Bacterial transfer RNAs (tRNAs) contain evolutionarily conserved sequences and modifications that ensure uniform binding to the ribosome and optimal translational accuracy despite differences in their aminoacyl attachments and anticodon nucleotide sequences. In the tRNA anticodon stem-loop, the anticodon sequence is correlated with a base pair in the anticodon loop (nucleotides 32 and 38) to tune the binding of each tRNA to the decoding center in the ribosome. Disruption of this correlation renders the ribosome unable to distinguish correct from incorrect tRNAs. The molecular basis for how these two tRNA features combine to ensure accurate decoding is unclear. Here, we solved structures of the bacterial ribosome containing either wild-type [Formula: see text] or [Formula: see text] containing a reversed 32-38 pair on cognate and near-cognate codons. Structures of wild-type [Formula: see text] bound to the ribosome reveal 23S ribosomal RNA (rRNA) nucleotide A1913 positional changes that are dependent on whether the codon-anticodon interaction is cognate or near cognate. Further, the 32-38 pair is destabilized in the context of a near-cognate codon-anticodon pair. Reversal of the pairing in [Formula: see text] ablates A1913 movement regardless of whether the interaction is cognate or near cognate. These results demonstrate that disrupting 32-38 and anticodon sequences alters interactions with the ribosome that directly contribute to misreading.

Authors+Show Affiliations

Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322. Department of Chemistry, Emory University, Atlanta, GA 30322.Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322.Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322; christine.m.dunham@emory.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32601241

Citation

Nguyen, Ha An, et al. "Disruption of Evolutionarily Correlated tRNA Elements Impairs Accurate Decoding." Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 28, 2020, pp. 16333-16338.
Nguyen HA, Sunita S, Dunham CM. Disruption of evolutionarily correlated tRNA elements impairs accurate decoding. Proc Natl Acad Sci USA. 2020;117(28):16333-16338.
Nguyen, H. A., Sunita, S., & Dunham, C. M. (2020). Disruption of evolutionarily correlated tRNA elements impairs accurate decoding. Proceedings of the National Academy of Sciences of the United States of America, 117(28), 16333-16338. https://doi.org/10.1073/pnas.2004170117
Nguyen HA, Sunita S, Dunham CM. Disruption of Evolutionarily Correlated tRNA Elements Impairs Accurate Decoding. Proc Natl Acad Sci USA. 2020 Jul 14;117(28):16333-16338. PubMed PMID: 32601241.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Disruption of evolutionarily correlated tRNA elements impairs accurate decoding. AU - Nguyen,Ha An, AU - Sunita,S, AU - Dunham,Christine M, Y1 - 2020/06/29/ PY - 2020/12/29/pmc-release PY - 2020/7/1/pubmed PY - 2020/7/1/medline PY - 2020/7/1/entrez KW - anticodon KW - miscoding KW - ribosome KW - structural biology KW - tRNA SP - 16333 EP - 16338 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 117 IS - 28 N2 - Bacterial transfer RNAs (tRNAs) contain evolutionarily conserved sequences and modifications that ensure uniform binding to the ribosome and optimal translational accuracy despite differences in their aminoacyl attachments and anticodon nucleotide sequences. In the tRNA anticodon stem-loop, the anticodon sequence is correlated with a base pair in the anticodon loop (nucleotides 32 and 38) to tune the binding of each tRNA to the decoding center in the ribosome. Disruption of this correlation renders the ribosome unable to distinguish correct from incorrect tRNAs. The molecular basis for how these two tRNA features combine to ensure accurate decoding is unclear. Here, we solved structures of the bacterial ribosome containing either wild-type [Formula: see text] or [Formula: see text] containing a reversed 32-38 pair on cognate and near-cognate codons. Structures of wild-type [Formula: see text] bound to the ribosome reveal 23S ribosomal RNA (rRNA) nucleotide A1913 positional changes that are dependent on whether the codon-anticodon interaction is cognate or near cognate. Further, the 32-38 pair is destabilized in the context of a near-cognate codon-anticodon pair. Reversal of the pairing in [Formula: see text] ablates A1913 movement regardless of whether the interaction is cognate or near cognate. These results demonstrate that disrupting 32-38 and anticodon sequences alters interactions with the ribosome that directly contribute to misreading. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/32601241/Disruption_of_evolutionarily_correlated_tRNA_elements_impairs_accurate_decoding L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=32601241 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.