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Evaluation of SARS-CoV-2 viral RNA in fecal samples.
Virol J. 2020 06 30; 17(1):86.VJ

Abstract

The need for timely establishment of a complete diagnostic protocol of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is demanded worldwide. We selected 15 positive novel coronavirus disease 19 (COVID-19) patients with mild or no symptom. Initially, fecal samples were negative in the 67% (10/15) of the cases, while 33% (5/10) of the cases were positive. After serial virus RNA testing, 73% (11/15) of the cases resulted positive to fecal specimens. In particular, 15 days after the first positive respiratory specimens test, 6 fecal specimens became positive for SARS-CoV-2 RNA, while 13 respiratory test returned negative result. In conclusion, qRT-PCR assays of fecal specimens, is an important step to control infection, suggesting that samples remained positive for SARS-CoV-2 RNA longer time then respiratory tract samples. Our results enhance the recent knowledge on this emerging infectious disease and offer suggestions for a more complete diagnostic strategy.

Authors+Show Affiliations

ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy.ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy. katia.margiotti@artemisia.it.ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy.ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy.ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy.ALTAMEDICA, Human Genetics laboratories, Altamedica Main Centre, Artemisia SpA, Viale Liegi 45, 00198, Rome, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32605577

Citation

Mesoraca, Alvaro, et al. "Evaluation of SARS-CoV-2 Viral RNA in Fecal Samples." Virology Journal, vol. 17, no. 1, 2020, p. 86.
Mesoraca A, Margiotti K, Viola A, et al. Evaluation of SARS-CoV-2 viral RNA in fecal samples. Virol J. 2020;17(1):86.
Mesoraca, A., Margiotti, K., Viola, A., Cima, A., Sparacino, D., & Giorlandino, C. (2020). Evaluation of SARS-CoV-2 viral RNA in fecal samples. Virology Journal, 17(1), 86. https://doi.org/10.1186/s12985-020-01359-1
Mesoraca A, et al. Evaluation of SARS-CoV-2 Viral RNA in Fecal Samples. Virol J. 2020 06 30;17(1):86. PubMed PMID: 32605577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of SARS-CoV-2 viral RNA in fecal samples. AU - Mesoraca,Alvaro, AU - Margiotti,Katia, AU - Viola,Antonella, AU - Cima,Antonella, AU - Sparacino,Davide, AU - Giorlandino,Claudio, Y1 - 2020/06/30/ PY - 2020/05/13/received PY - 2020/06/23/accepted PY - 2020/7/2/entrez PY - 2020/7/2/pubmed PY - 2020/7/21/medline KW - Acute respiratory syndrome coronavirus 2 (SARS-CoV-2) KW - Fecal specimens KW - Novel coronavirus disease 19 (COVID-19) KW - Respiratory specimens KW - SARS-CoV-2 viral RNA SP - 86 EP - 86 JF - Virology journal JO - Virol J VL - 17 IS - 1 N2 - The need for timely establishment of a complete diagnostic protocol of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is demanded worldwide. We selected 15 positive novel coronavirus disease 19 (COVID-19) patients with mild or no symptom. Initially, fecal samples were negative in the 67% (10/15) of the cases, while 33% (5/10) of the cases were positive. After serial virus RNA testing, 73% (11/15) of the cases resulted positive to fecal specimens. In particular, 15 days after the first positive respiratory specimens test, 6 fecal specimens became positive for SARS-CoV-2 RNA, while 13 respiratory test returned negative result. In conclusion, qRT-PCR assays of fecal specimens, is an important step to control infection, suggesting that samples remained positive for SARS-CoV-2 RNA longer time then respiratory tract samples. Our results enhance the recent knowledge on this emerging infectious disease and offer suggestions for a more complete diagnostic strategy. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/32605577/Evaluation_of_SARS_CoV_2_viral_RNA_in_fecal_samples_ DB - PRIME DP - Unbound Medicine ER -