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Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells.
Virol J. 2020 06 30; 17(1):87.VJ

Abstract

BACKGROUND

This study was designed to investigate the invasion of human papillomavirus (HPV) positive human cervical carcinoma cell lines in human leiomyoma-based extracellular matrices in vitro, and to test the suitability of the model for studying the irradiation effects on the cancer cell invasion.

METHODS

HPV positive cervical carcinoma cell lines SiHa and CaSki, and HPV negative squamous cell carcinoma cell line HSC-3 were used. CaSki cells contain around 600 copies of HPV 16 virus in the genome, whereas SiHa have only 1-2 copies per cell. Cells were analyzed using two different human tumor derived extracellular matrix methods (3D myoma disc model, and Myogel Transwell invasion assay). Cultures were irradiated with 4 Gy. Myoma invasion area and the depth of invasion were measured with ImageJ 1.51j8 software. Statistical analyses were performed with SPSS Statistics (IBM SPSS® Statistics 25).

RESULTS

All cells invaded through Myogel coated Transwell membranes and within myoma discs. In myoma discs, a difference in the invasion depth (p = 0.0001) but not in invasion area (p = 0.310) between the HPV positive cell lines was seen, since SiHa (less HPV) invaded slightly better than CaSki (more HPV). HSC-3 cells (HPV negative) invaded deepest (p = 0.048) than either of the HPV positive cell line cells. No difference was detected in the invasion area (p = 0.892) between HPV positive and HPV negative cells. The ionized radiation significantly reduced the invasion depth of HSC-3 (p = 0.008), SiHa (p = 0.0001) and CaSki (p = 0.005). No significant effect on the invasion area was detected in any of the cell lines. However, a significant difference was observed between SiHa and CaSki in the reduction of the invasion depth after radiation (p = 0.013) as the reduction was greater with SiHa than CaSki.

CONCLUSIONS

Both solid and gelatinous human leiomyoma-based extracellular matrix models were suitable platforms to study the invasion of HPV positive cervical carcinoma cells in vitro. SiHa cells with less HPV copy number cells invaded slightly better and were slightly more sensitive to irradiation than CaSki cells with high HPV copy number. However, there was no drastic differences between the invasion properties of these carcinoma cells.

Authors+Show Affiliations

Department of Oral Pathology and Oral Radiology, Institute of Dentistry, Faculty of Medicine, University of Turku, Lemminkäisenkatu 2, FIN-20520, Turku, Finland. heemtu@utu.fi. Department of Pathology, Turku University Hospital, Turku, Finland. heemtu@utu.fi.Translational Immunology Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland. Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.Translational Immunology Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland. Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland. Medical Research Center Oulu, Oulu University Hospital, Oulu, Finland. Cancer Research and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, Oulu, Finland. HUSLAB, Department of Pathology, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland.Department of Oral Pathology and Oral Radiology, Institute of Dentistry, Faculty of Medicine, University of Turku, Lemminkäisenkatu 2, FIN-20520, Turku, Finland. Department of Pathology, Turku University Hospital, Turku, Finland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32605632

Citation

Tuominen, Heidi, et al. "Human Myoma Tissue-based Extracellular Matrix Models for Testing the Effects of Irradiation On the HPV Positive Cells." Virology Journal, vol. 17, no. 1, 2020, p. 87.
Tuominen H, Al-Samadi A, Salo T, et al. Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells. Virol J. 2020;17(1):87.
Tuominen, H., Al-Samadi, A., Salo, T., & Rautava, J. (2020). Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells. Virology Journal, 17(1), 87. https://doi.org/10.1186/s12985-020-01367-1
Tuominen H, et al. Human Myoma Tissue-based Extracellular Matrix Models for Testing the Effects of Irradiation On the HPV Positive Cells. Virol J. 2020 06 30;17(1):87. PubMed PMID: 32605632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human myoma tissue-based extracellular matrix models for testing the effects of irradiation on the HPV positive cells. AU - Tuominen,Heidi, AU - Al-Samadi,Ahmed, AU - Salo,Tuula, AU - Rautava,Jaana, Y1 - 2020/06/30/ PY - 2019/12/13/received PY - 2020/06/23/accepted PY - 2020/7/2/entrez PY - 2020/7/2/pubmed PY - 2020/7/2/medline KW - Cell culture KW - Cell invasion KW - HPV KW - Ionizing radiation KW - Myogel KW - Myoma SP - 87 EP - 87 JF - Virology journal JO - Virol. J. VL - 17 IS - 1 N2 - BACKGROUND: This study was designed to investigate the invasion of human papillomavirus (HPV) positive human cervical carcinoma cell lines in human leiomyoma-based extracellular matrices in vitro, and to test the suitability of the model for studying the irradiation effects on the cancer cell invasion. METHODS: HPV positive cervical carcinoma cell lines SiHa and CaSki, and HPV negative squamous cell carcinoma cell line HSC-3 were used. CaSki cells contain around 600 copies of HPV 16 virus in the genome, whereas SiHa have only 1-2 copies per cell. Cells were analyzed using two different human tumor derived extracellular matrix methods (3D myoma disc model, and Myogel Transwell invasion assay). Cultures were irradiated with 4 Gy. Myoma invasion area and the depth of invasion were measured with ImageJ 1.51j8 software. Statistical analyses were performed with SPSS Statistics (IBM SPSS® Statistics 25). RESULTS: All cells invaded through Myogel coated Transwell membranes and within myoma discs. In myoma discs, a difference in the invasion depth (p = 0.0001) but not in invasion area (p = 0.310) between the HPV positive cell lines was seen, since SiHa (less HPV) invaded slightly better than CaSki (more HPV). HSC-3 cells (HPV negative) invaded deepest (p = 0.048) than either of the HPV positive cell line cells. No difference was detected in the invasion area (p = 0.892) between HPV positive and HPV negative cells. The ionized radiation significantly reduced the invasion depth of HSC-3 (p = 0.008), SiHa (p = 0.0001) and CaSki (p = 0.005). No significant effect on the invasion area was detected in any of the cell lines. However, a significant difference was observed between SiHa and CaSki in the reduction of the invasion depth after radiation (p = 0.013) as the reduction was greater with SiHa than CaSki. CONCLUSIONS: Both solid and gelatinous human leiomyoma-based extracellular matrix models were suitable platforms to study the invasion of HPV positive cervical carcinoma cells in vitro. SiHa cells with less HPV copy number cells invaded slightly better and were slightly more sensitive to irradiation than CaSki cells with high HPV copy number. However, there was no drastic differences between the invasion properties of these carcinoma cells. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/32605632/Human_myoma_tissue-based_extracellular_matrix_models_for_testing_the_effects_of_irradiation_on_the_HPV_positive_cells L2 - https://virologyj.biomedcentral.com/articles/10.1186/s12985-020-01367-1 DB - PRIME DP - Unbound Medicine ER -
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