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ERK Regulates NeuroD1-mediated Neurite Outgrowth via Proteasomal Degradation.
Exp Neurobiol. 2020 Jun 30; 29(3):189-206.EN

Abstract

Neurogenic differentiation 1 (NeuroD1) is a class B basic helix-loop-helix (bHLH) transcription factor and regulates differentiation and survival of neuronal and endocrine cells by means of several protein kinases, including extracellular signal-regulated kinase (ERK). However, the effect of phosphorylation on the functions of NeuroD1 by ERK has sparked controversy based on context-dependent differences across diverse species and cell types. Here, we evidenced that ERK-dependent phosphorylation controlled the stability of NeuroD1 and consequently, regulated proneural activity in neuronal cells. A null mutation at the ERK-dependent phosphorylation site, S274A, increased the half-life of NeuroD1 by blocking its ubiquitin-dependent proteasomal degradation. The S274A mutation did not interfere with either the nuclear translocation of NeuroD1 or its heterodimerization with E47, its ubiquitous partner and class A bHLH transcription factor. However, the S274A mutant increased transactivation of the E-box-mediated gene and neurite outgrowth in F11 neuroblastoma cells, compared to the wild-type NeuroD1. Transcriptome and Gene Ontology enrichment analyses indicated that genes involved in axonogenesis and dendrite development were downregulated in NeuroD1 knockout (KO) mice. Overexpression of the S274A mutant salvaged neurite outgrowth in NeuroD1-deficient mice, whereas neurite outgrowth was minimal with S274D, a phosphomimicking mutant. Our data indicated that a longer protein half-life enhanced the overall activity of NeuroD1 in stimulating downstream genes and neuronal differentiation. We propose that blocking ubiquitin-dependent proteasomal degradation may serve as a strategy to promote neuronal activity by stimulating the expression of neuron-specific genes in differentiating neurons.

Authors+Show Affiliations

Department of Anatomy, Ajou University School of Medicine, Suwon 16499, Korea. Department of Biomedical Sciences, Graduate School, Ajou University School of Medicine, Suwon 16499, Korea. Research Center, CelleBrain Ltd., Jeonju 54871, Korea.Department of Anatomy, Ajou University School of Medicine, Suwon 16499, Korea.Department of Anatomy, Ajou University School of Medicine, Suwon 16499, Korea. Department of Biomedical Sciences, Graduate School, Ajou University School of Medicine, Suwon 16499, Korea.Department of Biology, Life Science and Engineering Building, Boston University, Boston, MA 00215, USA.Department of Medicine and Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA.Department of Anatomy, Ajou University School of Medicine, Suwon 16499, Korea.Department of Anatomy, Ajou University School of Medicine, Suwon 16499, Korea.Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.Department of Anatomy, Ajou University School of Medicine, Suwon 16499, Korea. Department of Biomedical Sciences, Graduate School, Ajou University School of Medicine, Suwon 16499, Korea.Department of Anatomy, Ajou University School of Medicine, Suwon 16499, Korea. Department of Biomedical Sciences, Graduate School, Ajou University School of Medicine, Suwon 16499, Korea. Research Center, CelleBrain Ltd., Jeonju 54871, Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32606250

Citation

Lee, Tae-Young, et al. "ERK Regulates NeuroD1-mediated Neurite Outgrowth Via Proteasomal Degradation." Experimental Neurobiology, vol. 29, no. 3, 2020, pp. 189-206.
Lee TY, Cho IS, Bashyal N, et al. ERK Regulates NeuroD1-mediated Neurite Outgrowth via Proteasomal Degradation. Exp Neurobiol. 2020;29(3):189-206.
Lee, T. Y., Cho, I. S., Bashyal, N., Naya, F. J., Tsai, M. J., Yoon, J. S., Choi, J. M., Park, C. H., Kim, S. S., & Suh-Kim, H. (2020). ERK Regulates NeuroD1-mediated Neurite Outgrowth via Proteasomal Degradation. Experimental Neurobiology, 29(3), 189-206. https://doi.org/10.5607/en20021
Lee TY, et al. ERK Regulates NeuroD1-mediated Neurite Outgrowth Via Proteasomal Degradation. Exp Neurobiol. 2020 Jun 30;29(3):189-206. PubMed PMID: 32606250.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ERK Regulates NeuroD1-mediated Neurite Outgrowth via Proteasomal Degradation. AU - Lee,Tae-Young, AU - Cho,In-Su, AU - Bashyal,Narayan, AU - Naya,Francisco J, AU - Tsai,Ming-Jer, AU - Yoon,Jeong Seon, AU - Choi,Jung-Mi, AU - Park,Chang-Hwan, AU - Kim,Sung-Soo, AU - Suh-Kim,Haeyoung, PY - 2020/06/01/received PY - 2020/06/16/revised PY - 2020/06/16/accepted PY - 2020/7/2/pubmed PY - 2020/7/2/medline PY - 2020/7/2/entrez KW - Extracellular signal-regulated kinase KW - Neurite outgrowth KW - Neurogenic differentiation factor 1 KW - Phosphorylation SP - 189 EP - 206 JF - Experimental neurobiology JO - Exp Neurobiol VL - 29 IS - 3 N2 - Neurogenic differentiation 1 (NeuroD1) is a class B basic helix-loop-helix (bHLH) transcription factor and regulates differentiation and survival of neuronal and endocrine cells by means of several protein kinases, including extracellular signal-regulated kinase (ERK). However, the effect of phosphorylation on the functions of NeuroD1 by ERK has sparked controversy based on context-dependent differences across diverse species and cell types. Here, we evidenced that ERK-dependent phosphorylation controlled the stability of NeuroD1 and consequently, regulated proneural activity in neuronal cells. A null mutation at the ERK-dependent phosphorylation site, S274A, increased the half-life of NeuroD1 by blocking its ubiquitin-dependent proteasomal degradation. The S274A mutation did not interfere with either the nuclear translocation of NeuroD1 or its heterodimerization with E47, its ubiquitous partner and class A bHLH transcription factor. However, the S274A mutant increased transactivation of the E-box-mediated gene and neurite outgrowth in F11 neuroblastoma cells, compared to the wild-type NeuroD1. Transcriptome and Gene Ontology enrichment analyses indicated that genes involved in axonogenesis and dendrite development were downregulated in NeuroD1 knockout (KO) mice. Overexpression of the S274A mutant salvaged neurite outgrowth in NeuroD1-deficient mice, whereas neurite outgrowth was minimal with S274D, a phosphomimicking mutant. Our data indicated that a longer protein half-life enhanced the overall activity of NeuroD1 in stimulating downstream genes and neuronal differentiation. We propose that blocking ubiquitin-dependent proteasomal degradation may serve as a strategy to promote neuronal activity by stimulating the expression of neuron-specific genes in differentiating neurons. SN - 1226-2560 UR - https://www.unboundmedicine.com/medline/citation/32606250/ERK_Regulates_NeuroD1-mediated_Neurite_Outgrowth_via_Proteasomal_Degradation L2 - https://doi.org/10.5607/en20021 DB - PRIME DP - Unbound Medicine ER -
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