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Quantitative assessment of CD15 positive tissue eosinophils in Oral Squamous Cell Carcinoma: effects on mast cells and tumor angiogenesis.
Minerva Stomatol. 2020 Jun; 69(3):133-140.MS

Abstract

BACKGROUND

The present study determines to correlate eosinophil, mast cell and microvessel densities with the histopathological grades and clinical staging of Oral Squamous Cell Carcinoma (OSCC) cases, as the potential role of inflammatory mediators within tumor stroma remains debatable.

METHODS

The study sample comprised 60 cases consisting of 40 cases of Well to moderately differentiated OSCC (group 1) and 20 cases of poorly differentiated OSCC (group 2). Immunohistochemistry with anti-CD15 antibody and antifactor VIII antibody; and toluidine blue special stain were employed for the detection of eosinophils, microvessels, and mast cells, respectively.

RESULTS

The mean numbers of eosinophils, mast cells, and microvessels per high power field in group 1 and group 2 were 15.37±11.86 and 12.62±14.30, 6.00±4.84 and 4.51±4.51, 13.96±6.25 and 6.62±2.05, respectively. Eosinophil density had a positive correlation with both mast cell and microvessel density. Also, the correlation of primary tumor size (T status) with microvessel density was found to be statistically significant (P≤0.05).

CONCLUSIONS

The cohesive interpretation of the aforementioned mediators in OSCC suggested that while these variables correlate well with the differentiation of tumor, the quantification did not correlate with the clinical staging of the disease.

Authors+Show Affiliations

Department of Oral and Maxillofacial Pathology and Oral Microbiology, I.T.S Center for Dental Studies and Research, Muradnagar, India - sethiaashka90@gmail.com.Department of Oral and Maxillofacial Pathology and Oral Microbiology, I.T.S Center for Dental Studies and Research, Muradnagar, India.Department of Oral and Maxillofacial Pathology and Oral Microbiology, I.T.S Center for Dental Studies and Research, Muradnagar, India.Department of Oral Pathology, Microbiology and Forensic Odontology, Dental Institute, RIMS, Ranehi, India.Department of Oral and Maxillofacial Pathology and Oral Microbiology, I.T.S Center for Dental Studies and Research, Muradnagar, India.Department of Oral and Maxillofacial Pathology and Oral Microbiology, I.T.S Center for Dental Studies and Research, Muradnagar, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32610722

Citation

Sethi, Aashka, et al. "Quantitative Assessment of CD15 Positive Tissue Eosinophils in Oral Squamous Cell Carcinoma: Effects On Mast Cells and Tumor Angiogenesis." Minerva Stomatologica, vol. 69, no. 3, 2020, pp. 133-140.
Sethi A, Shetty DC, Rathore AS, et al. Quantitative assessment of CD15 positive tissue eosinophils in Oral Squamous Cell Carcinoma: effects on mast cells and tumor angiogenesis. Minerva Stomatol. 2020;69(3):133-140.
Sethi, A., Shetty, D. C., Rathore, A. S., Tandon, A., Juneja, S., & Gulati, N. (2020). Quantitative assessment of CD15 positive tissue eosinophils in Oral Squamous Cell Carcinoma: effects on mast cells and tumor angiogenesis. Minerva Stomatologica, 69(3), 133-140. https://doi.org/10.23736/S0026-4970.19.04285-7
Sethi A, et al. Quantitative Assessment of CD15 Positive Tissue Eosinophils in Oral Squamous Cell Carcinoma: Effects On Mast Cells and Tumor Angiogenesis. Minerva Stomatol. 2020;69(3):133-140. PubMed PMID: 32610722.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative assessment of CD15 positive tissue eosinophils in Oral Squamous Cell Carcinoma: effects on mast cells and tumor angiogenesis. AU - Sethi,Aashka, AU - Shetty,Devi C, AU - Rathore,Ajit S, AU - Tandon,Ankita, AU - Juneja,Saurabh, AU - Gulati,Nikita, PY - 2020/7/3/entrez PY - 2020/7/3/pubmed PY - 2020/7/4/medline SP - 133 EP - 140 JF - Minerva stomatologica JO - Minerva Stomatol VL - 69 IS - 3 N2 - BACKGROUND: The present study determines to correlate eosinophil, mast cell and microvessel densities with the histopathological grades and clinical staging of Oral Squamous Cell Carcinoma (OSCC) cases, as the potential role of inflammatory mediators within tumor stroma remains debatable. METHODS: The study sample comprised 60 cases consisting of 40 cases of Well to moderately differentiated OSCC (group 1) and 20 cases of poorly differentiated OSCC (group 2). Immunohistochemistry with anti-CD15 antibody and antifactor VIII antibody; and toluidine blue special stain were employed for the detection of eosinophils, microvessels, and mast cells, respectively. RESULTS: The mean numbers of eosinophils, mast cells, and microvessels per high power field in group 1 and group 2 were 15.37±11.86 and 12.62±14.30, 6.00±4.84 and 4.51±4.51, 13.96±6.25 and 6.62±2.05, respectively. Eosinophil density had a positive correlation with both mast cell and microvessel density. Also, the correlation of primary tumor size (T status) with microvessel density was found to be statistically significant (P≤0.05). CONCLUSIONS: The cohesive interpretation of the aforementioned mediators in OSCC suggested that while these variables correlate well with the differentiation of tumor, the quantification did not correlate with the clinical staging of the disease. SN - 1827-174X UR - https://www.unboundmedicine.com/medline/citation/32610722/Quantitative_assessment_of_CD15_positive_tissue_eosinophils_in_Oral_Squamous_Cell_Carcinoma:_effects_on_mast_cells_and_tumor_angiogenesis DB - PRIME DP - Unbound Medicine ER -