Tags

Type your tag names separated by a space and hit enter

Prevalence of Mycoplasma genitalium and antibiotic resistance-associated mutations in an Icelandic STI population, and comparison of the S-DiaMGTV and Aptima Mycoplasma genitalium assays for diagnosis.
J Clin Microbiol. 2020 Jul 01 [Online ahead of print]JC

Abstract

Objectives:

Mycoplasma genitalium (MG) is prevalent among attendees in sexually transmitted infections (STI) clinics and therapy is hampered by rapidly rising levels of resistance to azithromycin and moxifloxacin. In this study we evaluated, for the first time in Iceland, the prevalence of MG and azithromycin and moxifloxacin resistance-associated mutations, and assessed the diagnostic performance of the CE/IVD-marked S-DiaMGTV (Diagenode Diagnostics) versus the US FDA/CE/IVD-approved Aptima MG (AMG; Hologic) for MG detection.

Methods:

From October 2018 to January 2019, urine and vaginal swabs were provided by male and female attendees at Iceland's only STI clinic. Specimens were tested with S-DiaMGTV and AMG, and resistance-associated mutations were determined by 23S rRNA gene and parC sequencing. Demographic and clinical data were collected from patient records.

Results:

MG prevalence was 9.3% overall; 7.7% (38/491) among male and 10.9% (53/487) among female participants. Azithromycin and moxifloxacin resistance-associated mutations were found in 57.0% (45/79) and 0.0% (0/80) of evaluable specimens, respectively. Sensitivity was 72.5% and 100%, and specificity was 99.9% and 100% for S-DiaMGTV and AMG, respectively. No association was found between MG and symptoms of urethritis in men.

Conclusions:

Prevalence rates for MG and azithromycin resistance-associated genes in Iceland are among the highest reported in Europe. The significantly higher sensitivity of AMG over that of S-DiaMGTV can have important clinical implications. More information is urgently needed to clarify the significance of false-negative results obtained with S-DiaMGTV and other similarly performing, widely used real-time PCR methods for diagnosis and management of this sexually transmitted infection.

Authors+Show Affiliations

Department of Microbiology, Landspítali - The University Hospital of Iceland, Reykjavík, Iceland ingibjh@landspitali.is. Faculty of Medicine, University of Iceland, Reykjavík, Iceland.Department of Microbiology, Landspítali - The University Hospital of Iceland, Reykjavík, Iceland. Faculty of Medicine, University of Iceland, Reykjavík, Iceland.Department of Sexually Transmitted Diseases, Landspítali - The University Hospital of Iceland, Reykjavík, Iceland.Department of Microbiology, Landspítali - The University Hospital of Iceland, Reykjavík, Iceland.WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.Department of Microbiology, Landspítali - The University Hospital of Iceland, Reykjavík, Iceland.Department of Microbiology, Landspítali - The University Hospital of Iceland, Reykjavík, Iceland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32611793

Citation

Hilmarsdóttir, Ingibjörg, et al. "Prevalence of Mycoplasma Genitalium and Antibiotic Resistance-associated Mutations in an Icelandic STI Population, and Comparison of the S-DiaMGTV and Aptima Mycoplasma Genitalium Assays for Diagnosis." Journal of Clinical Microbiology, 2020.
Hilmarsdóttir I, Arnardóttir EM, Jóhannesdóttir ER, et al. Prevalence of Mycoplasma genitalium and antibiotic resistance-associated mutations in an Icelandic STI population, and comparison of the S-DiaMGTV and Aptima Mycoplasma genitalium assays for diagnosis. J Clin Microbiol. 2020.
Hilmarsdóttir, I., Arnardóttir, E. M., Jóhannesdóttir, E. R., Valsdóttir, F., Golparian, D., Hadad, R., Vigfússon, H. B., & Unemo, M. (2020). Prevalence of Mycoplasma genitalium and antibiotic resistance-associated mutations in an Icelandic STI population, and comparison of the S-DiaMGTV and Aptima Mycoplasma genitalium assays for diagnosis. Journal of Clinical Microbiology. https://doi.org/10.1128/JCM.01084-20
Hilmarsdóttir I, et al. Prevalence of Mycoplasma Genitalium and Antibiotic Resistance-associated Mutations in an Icelandic STI Population, and Comparison of the S-DiaMGTV and Aptima Mycoplasma Genitalium Assays for Diagnosis. J Clin Microbiol. 2020 Jul 1; PubMed PMID: 32611793.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of Mycoplasma genitalium and antibiotic resistance-associated mutations in an Icelandic STI population, and comparison of the S-DiaMGTV and Aptima Mycoplasma genitalium assays for diagnosis. AU - Hilmarsdóttir,Ingibjörg, AU - Arnardóttir,Eva Mjöll, AU - Jóhannesdóttir,Elísabet Reykdal, AU - Valsdóttir,Freyja, AU - Golparian,Daniel, AU - Hadad,Ronza, AU - Vigfússon,Hannes Bjarki, AU - Unemo,Magnus, Y1 - 2020/07/01/ PY - 2020/7/3/entrez JF - Journal of clinical microbiology JO - J. Clin. Microbiol. N2 - Objectives: Mycoplasma genitalium (MG) is prevalent among attendees in sexually transmitted infections (STI) clinics and therapy is hampered by rapidly rising levels of resistance to azithromycin and moxifloxacin. In this study we evaluated, for the first time in Iceland, the prevalence of MG and azithromycin and moxifloxacin resistance-associated mutations, and assessed the diagnostic performance of the CE/IVD-marked S-DiaMGTV (Diagenode Diagnostics) versus the US FDA/CE/IVD-approved Aptima MG (AMG; Hologic) for MG detection.Methods: From October 2018 to January 2019, urine and vaginal swabs were provided by male and female attendees at Iceland's only STI clinic. Specimens were tested with S-DiaMGTV and AMG, and resistance-associated mutations were determined by 23S rRNA gene and parC sequencing. Demographic and clinical data were collected from patient records.Results: MG prevalence was 9.3% overall; 7.7% (38/491) among male and 10.9% (53/487) among female participants. Azithromycin and moxifloxacin resistance-associated mutations were found in 57.0% (45/79) and 0.0% (0/80) of evaluable specimens, respectively. Sensitivity was 72.5% and 100%, and specificity was 99.9% and 100% for S-DiaMGTV and AMG, respectively. No association was found between MG and symptoms of urethritis in men.Conclusions: Prevalence rates for MG and azithromycin resistance-associated genes in Iceland are among the highest reported in Europe. The significantly higher sensitivity of AMG over that of S-DiaMGTV can have important clinical implications. More information is urgently needed to clarify the significance of false-negative results obtained with S-DiaMGTV and other similarly performing, widely used real-time PCR methods for diagnosis and management of this sexually transmitted infection. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/32611793/Prevalence_of_Mycoplasma_genitalium_and_antibiotic_resistance-associated_mutations_in_an_Icelandic_STI_population,_and_comparison_of_the_S-DiaMGTV_and_Aptima_Mycoplasma_genitalium_assays_for_diagnosis L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=32611793 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.