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Ribosome rescue activity of an Arabidopsis thaliana ArfB homolog.
Genes Genet Syst. 2020 Jul 02 [Online ahead of print]GG

Abstract

A homolog of the bacterial ribosome rescue factor ArfB was identified in Arabidopsis thaliana. The factor, named AtArfB for Arabidopsis thaliana ArfB, showed ribosome rescue activity in both in vivo and in vitro assays based on the bacterial translation system. As has been shown for ArfB, the ribosome rescue activity of AtArfB was dependent on the GGQ motif, the crucial motif for the function of class I release factors and ArfB. The C-terminal region of AtArfB was also important for its function. The N-terminal region of AtArfB, which is absent in bacterial ArfB, functioned as a transit peptide for chloroplast targeting in tobacco cells. These results strongly suggest that AtArfB is a ribosome rescue factor that functions in chloroplasts.

Authors+Show Affiliations

Graduate School of Natural Science and Technology, Okayama University.Graduate School of Integrated Science and Technology, Shizuoka University.Graduate School of Integrated Science and Technology, Shizuoka University. Research Institute of Green Science and Technology, Shizuoka University.Graduate School of Natural Science and Technology, Okayama University.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32611934

Citation

Nagao, Michiaki, et al. "Ribosome Rescue Activity of an Arabidopsis Thaliana ArfB Homolog." Genes & Genetic Systems, 2020.
Nagao M, Tsuchiya F, Motohashi R, et al. Ribosome rescue activity of an Arabidopsis thaliana ArfB homolog. Genes Genet Syst. 2020.
Nagao, M., Tsuchiya, F., Motohashi, R., & Abo, T. (2020). Ribosome rescue activity of an Arabidopsis thaliana ArfB homolog. Genes & Genetic Systems. https://doi.org/10.1266/ggs.20-00007
Nagao M, et al. Ribosome Rescue Activity of an Arabidopsis Thaliana ArfB Homolog. Genes Genet Syst. 2020 Jul 2; PubMed PMID: 32611934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ribosome rescue activity of an Arabidopsis thaliana ArfB homolog. AU - Nagao,Michiaki, AU - Tsuchiya,Fumina, AU - Motohashi,Reiko, AU - Abo,Tatsuhiko, Y1 - 2020/07/02/ PY - 2020/7/3/entrez KW - Arabidopsis thaliana KW - ArfB KW - chloroplast KW - ribosome rescue KW - translation JF - Genes & genetic systems JO - Genes Genet. Syst. N2 - A homolog of the bacterial ribosome rescue factor ArfB was identified in Arabidopsis thaliana. The factor, named AtArfB for Arabidopsis thaliana ArfB, showed ribosome rescue activity in both in vivo and in vitro assays based on the bacterial translation system. As has been shown for ArfB, the ribosome rescue activity of AtArfB was dependent on the GGQ motif, the crucial motif for the function of class I release factors and ArfB. The C-terminal region of AtArfB was also important for its function. The N-terminal region of AtArfB, which is absent in bacterial ArfB, functioned as a transit peptide for chloroplast targeting in tobacco cells. These results strongly suggest that AtArfB is a ribosome rescue factor that functions in chloroplasts. SN - 1880-5779 UR - https://www.unboundmedicine.com/medline/citation/32611934/Ribosome_rescue_activity_of_an_Arabidopsis_thaliana_ArfB_homolog L2 - https://dx.doi.org/10.1266/ggs.20-00007 DB - PRIME DP - Unbound Medicine ER -
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