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Intraepithelial T Cells Diverge by Intestinal Location as Pigs Age.
Front Immunol. 2020; 11:1139.FI

Abstract

T cells resident within the intestinal epithelium play a central role in barrier integrity and provide a first line of immune defense. Intraepithelial T cells (IETs) are among the earliest immune cells to populate and protect intestinal tissues, thereby giving them an important role in shaping gut health early in life. In pigs, IETs are poorly defined, and their maturation in young pigs has not been well-studied. Given the importance of IETs in contributing to early life and long-term intestinal health through interactions with epithelial cells, the microbiota, and additional environmental factors, a deeper characterization of IETs in pigs is warranted. The objective of this study was to analyze age- and intestinal location-dependent changes in IETs across multiple sites of the small and large intestine in pigs between 4- and 8-weeks of age. IETs increased in abundance over time and belonged to both γδ and αβ T cell lineages. Similar compositions of IETs were identified across intestinal sites in 4-week-old pigs, but compositions diverged between intestinal sites as pigs aged. CD2+CD8α+ γδ T cells and CD4-CD8α+ αβ T cells comprised >78% of total IETs at all intestinal locations and ages examined. Greater percentages of γδ IETs were present in large intestine compared to small intestine in older pigs. Small intestinal tissues had greater percentages of CD2+CD8α- γδ IETs, while CD2+CD8α+ γδ IET percentages were greater in the large intestine. Percentages of CD4-CD8α+ αβ IETs increased over time across all intestinal sites. Moreover, percentages of CD27+ cells decreased in ileum and large intestine over time, indicating increased IET activation as pigs aged. Percentages of CD27+ cells were also higher in small intestine compared to large intestine at later timepoints. Results herein emphasize 4- to 8-weeks of age as a critical window of IET maturation and suggest strong associations between intestinal location and age with IET heterogeneity in pigs.

Authors+Show Affiliations

Food Safety and Enteric Pathogens Research Unit, Agricultural Research Service, United States Department of Agriculture, National Animal Disease Center, Ames, IA, United States. Immunobiology Graduate Program, Iowa State University, Ames, IA, United States. Oak Ridge Institute for Science and Education, Agricultural Research Service Participation Program, Oak Ridge, TN, United States.Food Safety and Enteric Pathogens Research Unit, Agricultural Research Service, United States Department of Agriculture, National Animal Disease Center, Ames, IA, United States.Food Safety and Enteric Pathogens Research Unit, Agricultural Research Service, United States Department of Agriculture, National Animal Disease Center, Ames, IA, United States.Food Safety and Enteric Pathogens Research Unit, Agricultural Research Service, United States Department of Agriculture, National Animal Disease Center, Ames, IA, United States.Department of Animal Science, Iowa State University, Ames, IA, United States.Food Safety and Enteric Pathogens Research Unit, Agricultural Research Service, United States Department of Agriculture, National Animal Disease Center, Ames, IA, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32612605

Citation

Wiarda, Jayne E., et al. "Intraepithelial T Cells Diverge By Intestinal Location as Pigs Age." Frontiers in Immunology, vol. 11, 2020, p. 1139.
Wiarda JE, Trachsel JM, Bond ZF, et al. Intraepithelial T Cells Diverge by Intestinal Location as Pigs Age. Front Immunol. 2020;11:1139.
Wiarda, J. E., Trachsel, J. M., Bond, Z. F., Byrne, K. A., Gabler, N. K., & Loving, C. L. (2020). Intraepithelial T Cells Diverge by Intestinal Location as Pigs Age. Frontiers in Immunology, 11, 1139. https://doi.org/10.3389/fimmu.2020.01139
Wiarda JE, et al. Intraepithelial T Cells Diverge By Intestinal Location as Pigs Age. Front Immunol. 2020;11:1139. PubMed PMID: 32612605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraepithelial T Cells Diverge by Intestinal Location as Pigs Age. AU - Wiarda,Jayne E, AU - Trachsel,Julian M, AU - Bond,Zahra F, AU - Byrne,Kristen A, AU - Gabler,Nicholas K, AU - Loving,Crystal L, Y1 - 2020/06/16/ PY - 2020/03/03/received PY - 2020/05/11/accepted PY - 2020/7/3/entrez PY - 2020/7/3/pubmed PY - 2020/7/3/medline KW - T cell maturation KW - intestinal T cells KW - intestinal development KW - intraepithelial T cells KW - intraepithelial lymphocytes KW - pig T cells KW - pig intestine KW - porcine T cells SP - 1139 EP - 1139 JF - Frontiers in immunology JO - Front Immunol VL - 11 N2 - T cells resident within the intestinal epithelium play a central role in barrier integrity and provide a first line of immune defense. Intraepithelial T cells (IETs) are among the earliest immune cells to populate and protect intestinal tissues, thereby giving them an important role in shaping gut health early in life. In pigs, IETs are poorly defined, and their maturation in young pigs has not been well-studied. Given the importance of IETs in contributing to early life and long-term intestinal health through interactions with epithelial cells, the microbiota, and additional environmental factors, a deeper characterization of IETs in pigs is warranted. The objective of this study was to analyze age- and intestinal location-dependent changes in IETs across multiple sites of the small and large intestine in pigs between 4- and 8-weeks of age. IETs increased in abundance over time and belonged to both γδ and αβ T cell lineages. Similar compositions of IETs were identified across intestinal sites in 4-week-old pigs, but compositions diverged between intestinal sites as pigs aged. CD2+CD8α+ γδ T cells and CD4-CD8α+ αβ T cells comprised >78% of total IETs at all intestinal locations and ages examined. Greater percentages of γδ IETs were present in large intestine compared to small intestine in older pigs. Small intestinal tissues had greater percentages of CD2+CD8α- γδ IETs, while CD2+CD8α+ γδ IET percentages were greater in the large intestine. Percentages of CD4-CD8α+ αβ IETs increased over time across all intestinal sites. Moreover, percentages of CD27+ cells decreased in ileum and large intestine over time, indicating increased IET activation as pigs aged. Percentages of CD27+ cells were also higher in small intestine compared to large intestine at later timepoints. Results herein emphasize 4- to 8-weeks of age as a critical window of IET maturation and suggest strong associations between intestinal location and age with IET heterogeneity in pigs. SN - 1664-3224 UR - https://www.unboundmedicine.com/medline/citation/32612605/Intraepithelial_T_Cells_Diverge_by_Intestinal_Location_as_Pigs_Age L2 - https://doi.org/10.3389/fimmu.2020.01139 DB - PRIME DP - Unbound Medicine ER -
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