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Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China.
Int J Parasitol Parasites Wildl. 2020 Aug; 12:181-184.IJ

Abstract

Cryptosporidium is a ubiquitous protozoan in human and animals. To investigate the genetic diversity of Cryptosporidium spp. in alpaca (Vicugna pacos) in China, 484 fecal samples from alpacas were collected at nine sites, and Cryptosporidium spp. were screened with PCR amplification of the small subunit ribosome RNA (SSU rRNA) locus. Cryptosporidium spp. infected 2.9% (14/484) of the alpacas. Of the nine collection sites, two were positive for Cryptosporidium, Wensu (3.0%, 3/100) and Qinghe (31.4%, 11/35). Three Cryptosporidium species were identified: C. parvum (n = 2), C. ubiquitum (n = 1), and C. occultus (n = 11). Cryptosporidium parvum and C. ubiquitum were further subtyped with the 60-kDa glycoprotein locus (gp60). The two C. parvum isolates were subtype IIdA15G1, but the one C. ubiquitum isolate was not subtyped successfully. A phylogenetic analysis indicated that the Cryptosporidium isolates clustered with previously identified species. To our knowledge, this is the first report of Cryptosporidium infections in alpacas in China and provides baseline data for the study of Cryptosporidium in alpacas in China.

Authors+Show Affiliations

College of Animal Science, Tarim University, Alar, Xinjiang, 843300, China.Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, China.College of Animal Science, Tarim University, Alar, Xinjiang, 843300, China.College of Animal Science, Tarim University, Alar, Xinjiang, 843300, China.College of Animal Science, Tarim University, Alar, Xinjiang, 843300, China.College of Animal Science, Tarim University, Alar, Xinjiang, 843300, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32612927

Citation

Zhang, Qiyuan, et al. "Molecular Identification of Cryptosporidium Spp. in Alpacas (Vicugna Pacos) in China." International Journal for Parasitology. Parasites and Wildlife, vol. 12, 2020, pp. 181-184.
Zhang Q, Li J, Li Z, et al. Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China. Int J Parasitol Parasites Wildl. 2020;12:181-184.
Zhang, Q., Li, J., Li, Z., Xu, C., Hou, M., & Qi, M. (2020). Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China. International Journal for Parasitology. Parasites and Wildlife, 12, 181-184. https://doi.org/10.1016/j.ijppaw.2020.06.007
Zhang Q, et al. Molecular Identification of Cryptosporidium Spp. in Alpacas (Vicugna Pacos) in China. Int J Parasitol Parasites Wildl. 2020;12:181-184. PubMed PMID: 32612927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular identification of Cryptosporidium spp. in alpacas (Vicugna pacos) in China. AU - Zhang,Qiyuan, AU - Li,Junqiang, AU - Li,Zhiguo, AU - Xu,Chunyan, AU - Hou,Minyu, AU - Qi,Meng, Y1 - 2020/06/20/ PY - 2020/05/11/received PY - 2020/06/14/revised PY - 2020/06/15/accepted PY - 2020/7/3/entrez PY - 2020/7/3/pubmed PY - 2020/7/3/medline KW - Alpaca KW - China KW - Cryptosporidium KW - Molecular identification SP - 181 EP - 184 JF - International journal for parasitology. Parasites and wildlife JO - Int J Parasitol Parasites Wildl VL - 12 N2 - Cryptosporidium is a ubiquitous protozoan in human and animals. To investigate the genetic diversity of Cryptosporidium spp. in alpaca (Vicugna pacos) in China, 484 fecal samples from alpacas were collected at nine sites, and Cryptosporidium spp. were screened with PCR amplification of the small subunit ribosome RNA (SSU rRNA) locus. Cryptosporidium spp. infected 2.9% (14/484) of the alpacas. Of the nine collection sites, two were positive for Cryptosporidium, Wensu (3.0%, 3/100) and Qinghe (31.4%, 11/35). Three Cryptosporidium species were identified: C. parvum (n = 2), C. ubiquitum (n = 1), and C. occultus (n = 11). Cryptosporidium parvum and C. ubiquitum were further subtyped with the 60-kDa glycoprotein locus (gp60). The two C. parvum isolates were subtype IIdA15G1, but the one C. ubiquitum isolate was not subtyped successfully. A phylogenetic analysis indicated that the Cryptosporidium isolates clustered with previously identified species. To our knowledge, this is the first report of Cryptosporidium infections in alpacas in China and provides baseline data for the study of Cryptosporidium in alpacas in China. SN - 2213-2244 UR - https://www.unboundmedicine.com/medline/citation/32612927/Molecular_identification_of_Cryptosporidium_spp._in_alpacas_(Vicugna_pacos)_in_China L2 - https://linkinghub.elsevier.com/retrieve/pii/S2213-2244(20)30062-6 DB - PRIME DP - Unbound Medicine ER -
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