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Comparative proteomics of geographically distinct saw-scaled viper (Echis carinatus) venoms from India.
Toxicon X. 2020 Sep; 7:100048.TX

Abstract

Snakebite is a socio-economic problem in tropical countries and it is exacerbated by geographical venom variation of snakes. We investigated on venom variation in geographically distinct populations of Echis carinatus from three ecologically distinct regions: Tamil Nadu (ECVTN), Goa (ECVGO), and Rajasthan (ECVRAJ). Venom was fractionated by RP-HPLC, combined with SDS-PAGE, and subjected to tandem mass spectrometry. Toxins were identified, and their relative abundance was estimated. Using NCBI database of Echis genus, we queried the MS/MS spectra, and found 69, 38 and 38 proteins in ECVTN, ECVGO and ECVRAJ respectively, belonging to 8-10 different toxin families. The differences in the venom profiles were due to change in the relative composition of the toxin families. Snake venom metalloproteinase (svMP), Snaclecs and Phospholipase A2 (PLA2) were the major venom components in all the venoms. Heteromeric Disintegrins were found in ECVTN and absent in other venoms. ECVRAJ showed higher abundance of low-molecular-weight (>30 kDa) proteins than ECVTN and ECVGO. Cysteine-rich venom protein (CRISP) was highest in ECVRAJ (7.34%), followed by ECVTN (0.01%) and in ECVGO, it was not detected. These findings highlight the need for evaluating the efficacy of the polyvalent anti-venom to neutralize the toxins from geographically distinct venoms of E. carinatus.

Authors+Show Affiliations

CSIR-Centre for Cellular and Molecular Biology, Laboratory for the Conservation of Endangered Species, Hyderabad, India.CSIR-Centre for Cellular and Molecular Biology, Laboratory for the Conservation of Endangered Species, Hyderabad, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32613195

Citation

Bhatia, Siddharth, and Karthikeyan Vasudevan. "Comparative Proteomics of Geographically Distinct Saw-scaled Viper (Echis Carinatus) Venoms From India." Toxicon: X, vol. 7, 2020, p. 100048.
Bhatia S, Vasudevan K. Comparative proteomics of geographically distinct saw-scaled viper (Echis carinatus) venoms from India. Toxicon: X. 2020;7:100048.
Bhatia, S., & Vasudevan, K. (2020). Comparative proteomics of geographically distinct saw-scaled viper (Echis carinatus) venoms from India. Toxicon: X, 7, 100048. https://doi.org/10.1016/j.toxcx.2020.100048
Bhatia S, Vasudevan K. Comparative Proteomics of Geographically Distinct Saw-scaled Viper (Echis Carinatus) Venoms From India. Toxicon: X. 2020;7:100048. PubMed PMID: 32613195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative proteomics of geographically distinct saw-scaled viper (Echis carinatus) venoms from India. AU - Bhatia,Siddharth, AU - Vasudevan,Karthikeyan, Y1 - 2020/06/23/ PY - 2020/02/26/received PY - 2020/06/09/revised PY - 2020/06/17/accepted PY - 2020/7/3/entrez PY - 2020/7/3/pubmed PY - 2020/7/3/medline KW - De-complexation KW - Envenomation KW - Mass spectrometry KW - RP-HPLC KW - Snake venom KW - Toxins KW - Venomics SP - 100048 EP - 100048 JF - Toxicon: X VL - 7 N2 - Snakebite is a socio-economic problem in tropical countries and it is exacerbated by geographical venom variation of snakes. We investigated on venom variation in geographically distinct populations of Echis carinatus from three ecologically distinct regions: Tamil Nadu (ECVTN), Goa (ECVGO), and Rajasthan (ECVRAJ). Venom was fractionated by RP-HPLC, combined with SDS-PAGE, and subjected to tandem mass spectrometry. Toxins were identified, and their relative abundance was estimated. Using NCBI database of Echis genus, we queried the MS/MS spectra, and found 69, 38 and 38 proteins in ECVTN, ECVGO and ECVRAJ respectively, belonging to 8-10 different toxin families. The differences in the venom profiles were due to change in the relative composition of the toxin families. Snake venom metalloproteinase (svMP), Snaclecs and Phospholipase A2 (PLA2) were the major venom components in all the venoms. Heteromeric Disintegrins were found in ECVTN and absent in other venoms. ECVRAJ showed higher abundance of low-molecular-weight (>30 kDa) proteins than ECVTN and ECVGO. Cysteine-rich venom protein (CRISP) was highest in ECVRAJ (7.34%), followed by ECVTN (0.01%) and in ECVGO, it was not detected. These findings highlight the need for evaluating the efficacy of the polyvalent anti-venom to neutralize the toxins from geographically distinct venoms of E. carinatus. SN - 2590-1710 UR - https://www.unboundmedicine.com/medline/citation/32613195/Comparative_proteomics_of_geographically_distinct_saw_scaled_viper__Echis_carinatus__venoms_from_India_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2590-1710(20)30026-6 DB - PRIME DP - Unbound Medicine ER -
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