Periodically Ordered, Nuclease-Resistant DNA Nanowires Decorated with Cell-Specific Aptamers as Selective Theranostic Agents.Angew Chem Int Ed Engl. 2020 Jul 02 [Online ahead of print]AC
DNA nanostructures have shown potentials in cancer therapy. However, current clinical practice is hampered by the difficulty to deliver them into cancer cells and susceptibility to nuclease degradation. To overcome these limitations, we report herein a periodically-ordered nick-hidden DNA nanowire (NW) with high serum stability and active targeting functionality. The inner core is made of multiple-connected DNA double helices and the outer shell is composed of regularly-arranged standing-up hairpin aptamers. All terminals of the components are hidden from nuclease attacks while the target-binding sites are exposed to allow delivery to the cancer target. The DNA NW remains intact over 24-h incubation in serum solution. Animal imaging and cell apoptosis show that the anticancer drugs formulated NW displays long blood-circulation time and high specificity in inducing cancer cell apoptosis. Treatment of tumor-bearing mice validates in vivo therapeutic efficacy, demonstrating an ideal theranostic vehicle for targeted imaging and therapy of cancers.