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Nuclear Factor κB/MicroRNA-155 Upregulates the Expression Pattern of Cytokines in Regulating the Relapse of Chronic Sinusitis with Nasal Polyps and the Underlying Mechanism of Glucocorticoid.
Med Sci Monit. 2020 Jul 02; 26:e923618.MS

Abstract

BACKGROUND

The aim of this study was to explore the upregulated nuclear factor kappaB (NF-kappaB)/microRNA-155 (miR-155) in regulating inflammatory responses and relapse of chronic rhinosinusitis (CRS) with nasal polyps (NP), which underlies the molecular mechanism of glucocorticoid treatment. MATERIAL AND

METHODS

The study recruited 25 patients with eosinophilic (Eos) CRSwNP, 25 patients with Non-Eos CRSwNP, 25 patients with CRS without NP (CRSsNP) and 30 patients with nasal septum deviation (control group). The expression of NF-kappaB/miR-155 and inflammatory cytokines was detected in epithelial tissue specimens. Additionally, a mouse model of Eos CRSwNP was established, and the mice were treated by NF-kappaB inhibitor, miR-155 antagomir, or dexamethasone (DEX) to explore the role of NF-kB/miR-155 and the anti-inflammatory effects of glucocorticoid treatment.

RESULTS

Results showed that the expression level of NF-kappaB/miR-155 was significantly elevated in the Eos CRSwNP group, accompanied by the upregulation of cytokines: tumor necrosis factor (TNF)-alpha, interleukin (IL)-1, IL-4, IL-5 (P<0.05) compared with the control group, the CRSsNP group or the Non-Eos CRSwNP group. The upregulation of NF-kappaB/miR-155 increased inflammatory mediator cyclooxygenase2 (COX2) while decreasing anti-inflammatory mediator Src homology-2 domain-containing inositol 5-phosphatase 1 (SOCS1), which resulted in the aberrant expression pattern of cytokines in the mice model. DEX treatment inhibited the expression of cytokines and decreased the relapse rate of Eos CRSwNP via inhibiting NF-kappaB/miR-155 (P<0.05).

CONCLUSIONS

The upregulation of NF-kappaB/miR-155 was crucial in mediating the aberrant expression of inflammatory cytokines in Eos CRSwNP. This molecular mechanism is a concern with the high relapse rate of Eos CRSwNP. However, glucocorticoid treatment inhibited the relapse of CRSwNP via downregulation of NF-kappaB/miR-155.

Authors+Show Affiliations

Department of Otorhinolaryngology, The Second Affiliated Hospital of Suzhou University, Suzhou, Jiangsu, China (mainland).Department of Pathology, The Second Affiliated Hospital of Suzhou University, Suzhou, Jiangsu, China (mainland).Department of Radiology, The Second Affiliated Hospital of Suzhou University, Suzhou, Jiangsu, China (mainland).Department of Otorhinolaryngology, The Second Affiliated Hospital of Suzhou University, Suzhou, Jiangsu, China (mainland).

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32614806

Citation

Du, Jianbin, et al. "Nuclear Factor κB/MicroRNA-155 Upregulates the Expression Pattern of Cytokines in Regulating the Relapse of Chronic Sinusitis With Nasal Polyps and the Underlying Mechanism of Glucocorticoid." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 26, 2020, pp. e923618.
Du J, Lv H, Dou X, et al. Nuclear Factor κB/MicroRNA-155 Upregulates the Expression Pattern of Cytokines in Regulating the Relapse of Chronic Sinusitis with Nasal Polyps and the Underlying Mechanism of Glucocorticoid. Med Sci Monit. 2020;26:e923618.
Du, J., Lv, H., Dou, X., & Cao, Z. (2020). Nuclear Factor κB/MicroRNA-155 Upregulates the Expression Pattern of Cytokines in Regulating the Relapse of Chronic Sinusitis with Nasal Polyps and the Underlying Mechanism of Glucocorticoid. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e923618. https://doi.org/10.12659/MSM.923618
Du J, et al. Nuclear Factor κB/MicroRNA-155 Upregulates the Expression Pattern of Cytokines in Regulating the Relapse of Chronic Sinusitis With Nasal Polyps and the Underlying Mechanism of Glucocorticoid. Med Sci Monit. 2020 Jul 2;26:e923618. PubMed PMID: 32614806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nuclear Factor κB/MicroRNA-155 Upregulates the Expression Pattern of Cytokines in Regulating the Relapse of Chronic Sinusitis with Nasal Polyps and the Underlying Mechanism of Glucocorticoid. AU - Du,Jianbin, AU - Lv,Haijun, AU - Dou,Xin, AU - Cao,Zhongsheng, Y1 - 2020/07/02/ PY - 2020/7/3/entrez PY - 2020/7/3/pubmed PY - 2020/7/3/medline SP - e923618 EP - e923618 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med. Sci. Monit. VL - 26 N2 - BACKGROUND The aim of this study was to explore the upregulated nuclear factor kappaB (NF-kappaB)/microRNA-155 (miR-155) in regulating inflammatory responses and relapse of chronic rhinosinusitis (CRS) with nasal polyps (NP), which underlies the molecular mechanism of glucocorticoid treatment. MATERIAL AND METHODS The study recruited 25 patients with eosinophilic (Eos) CRSwNP, 25 patients with Non-Eos CRSwNP, 25 patients with CRS without NP (CRSsNP) and 30 patients with nasal septum deviation (control group). The expression of NF-kappaB/miR-155 and inflammatory cytokines was detected in epithelial tissue specimens. Additionally, a mouse model of Eos CRSwNP was established, and the mice were treated by NF-kappaB inhibitor, miR-155 antagomir, or dexamethasone (DEX) to explore the role of NF-kB/miR-155 and the anti-inflammatory effects of glucocorticoid treatment. RESULTS Results showed that the expression level of NF-kappaB/miR-155 was significantly elevated in the Eos CRSwNP group, accompanied by the upregulation of cytokines: tumor necrosis factor (TNF)-alpha, interleukin (IL)-1, IL-4, IL-5 (P<0.05) compared with the control group, the CRSsNP group or the Non-Eos CRSwNP group. The upregulation of NF-kappaB/miR-155 increased inflammatory mediator cyclooxygenase2 (COX2) while decreasing anti-inflammatory mediator Src homology-2 domain-containing inositol 5-phosphatase 1 (SOCS1), which resulted in the aberrant expression pattern of cytokines in the mice model. DEX treatment inhibited the expression of cytokines and decreased the relapse rate of Eos CRSwNP via inhibiting NF-kappaB/miR-155 (P<0.05). CONCLUSIONS The upregulation of NF-kappaB/miR-155 was crucial in mediating the aberrant expression of inflammatory cytokines in Eos CRSwNP. This molecular mechanism is a concern with the high relapse rate of Eos CRSwNP. However, glucocorticoid treatment inhibited the relapse of CRSwNP via downregulation of NF-kappaB/miR-155. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/32614806/Nuclear_Factor_κB/MicroRNA-155_Upregulates_the_Expression_Pattern_of_Cytokines_in_Regulating_the_Relapse_of_Chronic_Sinusitis_with_Nasal_Polyps_and_the_Underlying_Mechanism_of_Glucocorticoid L2 - https://www.medscimonit.com/download/index/idArt/923618 DB - PRIME DP - Unbound Medicine ER -
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