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Precipitation-based colorimetric multiplex immunoassay in hydrogel particles.
Lab Chip. 2020 Aug 21; 20(16):2841-2850.LC

Abstract

Despite a growing demand for more accessible diagnostic technologies, current methods struggle to simultaneously detect multiple analytes with acceptable sensitivity and portability. Colorimetric assays have been widely used due to their simplicity of signal readout, but the lack of multiplexibility has been a perpetual constraint. Meanwhile, particle-based assays offer multiplex detection by assigning an identity code to each analyte, but they often require lab-based equipment unsuitable for portable diagnostics. Here, by merging the two approaches, this paper reports a colorimetric multiplex immunoassay based on hydrogel microparticles that achieves the best of both worlds. The low-cost portable multiplex assay demonstrates sensitivities as high as and dynamic ranges greater than the lab-based enzyme-linked immunosorbent assay (ELISA). These critical advances are made possible by local precipitation and amplification of insoluble colour dyes inside the hydrogel networks. For the first time, enzymatic accumulation of colour dyes in hydrogel particles is reported and the kinetics of colour development is characterized in this work. By taking advantage of the colour signals in the visible spectrum, the hydrogel microparticles were imaged and analysed using low-cost portable devices. The colorimetric multiplex immunoassay was used to successfully detect three target biomarkers of preeclampsia and validated clinically using healthy and patient-derived plasma samples.

Authors+Show Affiliations

Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea. bong98@korea.ac.kr.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32614938

Citation

Roh, Yoon Ho, et al. "Precipitation-based Colorimetric Multiplex Immunoassay in Hydrogel Particles." Lab On a Chip, vol. 20, no. 16, 2020, pp. 2841-2850.
Roh YH, Lee HJ, Kim JY, et al. Precipitation-based colorimetric multiplex immunoassay in hydrogel particles. Lab Chip. 2020;20(16):2841-2850.
Roh, Y. H., Lee, H. J., Kim, J. Y., Kim, H. U., Kim, S. M., & Bong, K. W. (2020). Precipitation-based colorimetric multiplex immunoassay in hydrogel particles. Lab On a Chip, 20(16), 2841-2850. https://doi.org/10.1039/d0lc00325e
Roh YH, et al. Precipitation-based Colorimetric Multiplex Immunoassay in Hydrogel Particles. Lab Chip. 2020 Aug 21;20(16):2841-2850. PubMed PMID: 32614938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Precipitation-based colorimetric multiplex immunoassay in hydrogel particles. AU - Roh,Yoon Ho, AU - Lee,Hyun Jee, AU - Kim,Ju Yeon, AU - Kim,Hyeon Ung, AU - Kim,Sun Min, AU - Bong,Ki Wan, Y1 - 2020/07/02/ PY - 2020/7/3/pubmed PY - 2020/7/3/medline PY - 2020/7/3/entrez SP - 2841 EP - 2850 JF - Lab on a chip JO - Lab Chip VL - 20 IS - 16 N2 - Despite a growing demand for more accessible diagnostic technologies, current methods struggle to simultaneously detect multiple analytes with acceptable sensitivity and portability. Colorimetric assays have been widely used due to their simplicity of signal readout, but the lack of multiplexibility has been a perpetual constraint. Meanwhile, particle-based assays offer multiplex detection by assigning an identity code to each analyte, but they often require lab-based equipment unsuitable for portable diagnostics. Here, by merging the two approaches, this paper reports a colorimetric multiplex immunoassay based on hydrogel microparticles that achieves the best of both worlds. The low-cost portable multiplex assay demonstrates sensitivities as high as and dynamic ranges greater than the lab-based enzyme-linked immunosorbent assay (ELISA). These critical advances are made possible by local precipitation and amplification of insoluble colour dyes inside the hydrogel networks. For the first time, enzymatic accumulation of colour dyes in hydrogel particles is reported and the kinetics of colour development is characterized in this work. By taking advantage of the colour signals in the visible spectrum, the hydrogel microparticles were imaged and analysed using low-cost portable devices. The colorimetric multiplex immunoassay was used to successfully detect three target biomarkers of preeclampsia and validated clinically using healthy and patient-derived plasma samples. SN - 1473-0189 UR - https://www.unboundmedicine.com/medline/citation/32614938/Precipitation-based_colorimetric_multiplex_immunoassay_in_hydrogel_particles L2 - https://doi.org/10.1039/d0lc00325e DB - PRIME DP - Unbound Medicine ER -
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