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MicroRNAs 155-5p, 122-5p and 181a-5p identify patients with graft dysfunction due to T-cell mediated rejection after liver transplantation.
Liver Transpl. 2020 Jul 02 [Online ahead of print]LT

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that can be detected in plasma and whose expression is associated with pathological processes. The role of miRNAs in the non-invasive diagnosis of T-cell mediated rejection (TCMR) after liver transplantation is unclear. Thus, we aimed to assess the effectiveness of a panel of four miRNAs (155-5p, 122-5p, 181a-5p and 148-3p) in diagnosing TCMR in liver transplant recipients with graft dysfunction (GD), and compare its accuracy with previously published tests for diagnosing TCMR based on routine laboratory parameters. From a prospective cohort of 145 patients followed during the first year after transplant, 49 developed GD and underwent a liver biopsy and plasma collection for miRNA analysis using qPCR. Patients with GD due to TCMR (n=21) exhibited significantly higher (p<0.001) expression of miRNA 155-5p (2.05 vs 0.07), 122-5p (19.36 vs 1.66) and 181a-5p (1.33 vs 0.37) compared to those with GD from other causes (n=28). The AUROC of miRNAs 155-5p, 122-5p and 181a-5p for the diagnosis of TCMR was 0.87, 0.91 and 0.89 respectively, significantly higher than those of the other non-invasive tests (p<0.001). Furthermore, miRNA 155-5p identified all patients who presented TCMR during the first two weeks after transplant.

CONCLUSION:

miRNA plasmatic expression differentiates TCMR from other causes of GD in patients who have undergone liver transplantation and may be useful tools in clinical practice.

Authors+Show Affiliations

Liver Transplant Unit, Hospital Clínic Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), CIBERehd, University of Barcelona, Barcelona, Spain.Pharmacology and Toxicology, Biochemistry and Molecular Genetics, Biochemical Diagnostic Centre (CDB) Hospital Clínic Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), CIBERehd, University of Barcelona, Barcelona, Spain.Medical Statistics Core Facility, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) and Hospital Clinic, Barcelona, Spain. Biostatistics Unit, Faculty of Medicine, Universitat Autònoma de Barcelona, Barcelona, Spain.Pathology Department, Biochemical Diagnostic Centre (CDB), Hospital Clínic Barcelona, Institut d'Investigacions Biomèdiques August Pi I Sunyer (IDIBAPS), CIBERehd, University of Barcelona, Barcelona, Spain.Liver Transplant Unit, Hospital Clínic Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), CIBERehd, University of Barcelona, Barcelona, Spain.Liver Transplant Unit, Hospital Clínic Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), CIBERehd, University of Barcelona, Barcelona, Spain.Liver Transplant Unit, Hospital Clínic Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), CIBERehd, University of Barcelona, Barcelona, Spain.Pharmacology and Toxicology, Biochemistry and Molecular Genetics, Biochemical Diagnostic Centre (CDB) Hospital Clínic Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), CIBERehd, University of Barcelona, Barcelona, Spain.Liver Transplant Unit, Hospital Clínic Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), CIBERehd, University of Barcelona, Barcelona, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32615025

Citation

Ruiz, Pablo, et al. "MicroRNAs 155-5p, 122-5p and 181a-5p Identify Patients With Graft Dysfunction Due to T-cell Mediated Rejection After Liver Transplantation." Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 2020.
Ruiz P, Millán O, Ríos J, et al. MicroRNAs 155-5p, 122-5p and 181a-5p identify patients with graft dysfunction due to T-cell mediated rejection after liver transplantation. Liver Transpl. 2020.
Ruiz, P., Millán, O., Ríos, J., Díaz, A., Sastre, L., Colmenero, J., Crespo, G., Brunet, M., & Navasa, M. (2020). MicroRNAs 155-5p, 122-5p and 181a-5p identify patients with graft dysfunction due to T-cell mediated rejection after liver transplantation. Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society. https://doi.org/10.1002/lt.25842
Ruiz P, et al. MicroRNAs 155-5p, 122-5p and 181a-5p Identify Patients With Graft Dysfunction Due to T-cell Mediated Rejection After Liver Transplantation. Liver Transpl. 2020 Jul 2; PubMed PMID: 32615025.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MicroRNAs 155-5p, 122-5p and 181a-5p identify patients with graft dysfunction due to T-cell mediated rejection after liver transplantation. AU - Ruiz,Pablo, AU - Millán,Olga, AU - Ríos,Jose, AU - Díaz,Alba, AU - Sastre,Lydia, AU - Colmenero,Jordi, AU - Crespo,Gonzalo, AU - Brunet,Mercè, AU - Navasa,Miquel, Y1 - 2020/07/02/ PY - 2020/7/3/entrez PY - 2020/7/3/pubmed PY - 2020/7/3/medline KW - Biomarkers KW - Diagnostic KW - Immunology KW - Immunosuppression KW - Translational JF - Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society JO - Liver Transpl. N2 - MicroRNAs (miRNAs) are small non-coding RNAs that can be detected in plasma and whose expression is associated with pathological processes. The role of miRNAs in the non-invasive diagnosis of T-cell mediated rejection (TCMR) after liver transplantation is unclear. Thus, we aimed to assess the effectiveness of a panel of four miRNAs (155-5p, 122-5p, 181a-5p and 148-3p) in diagnosing TCMR in liver transplant recipients with graft dysfunction (GD), and compare its accuracy with previously published tests for diagnosing TCMR based on routine laboratory parameters. From a prospective cohort of 145 patients followed during the first year after transplant, 49 developed GD and underwent a liver biopsy and plasma collection for miRNA analysis using qPCR. Patients with GD due to TCMR (n=21) exhibited significantly higher (p<0.001) expression of miRNA 155-5p (2.05 vs 0.07), 122-5p (19.36 vs 1.66) and 181a-5p (1.33 vs 0.37) compared to those with GD from other causes (n=28). The AUROC of miRNAs 155-5p, 122-5p and 181a-5p for the diagnosis of TCMR was 0.87, 0.91 and 0.89 respectively, significantly higher than those of the other non-invasive tests (p<0.001). Furthermore, miRNA 155-5p identified all patients who presented TCMR during the first two weeks after transplant. CONCLUSION: miRNA plasmatic expression differentiates TCMR from other causes of GD in patients who have undergone liver transplantation and may be useful tools in clinical practice. SN - 1527-6473 UR - https://www.unboundmedicine.com/medline/citation/32615025/MicroRNAs_155-5p,_122-5p_and_181a-5p_identify_patients_with_graft_dysfunction_due_to_T-cell_mediated_rejection_after_liver_transplantation L2 - https://doi.org/10.1002/lt.25842 DB - PRIME DP - Unbound Medicine ER -
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