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Nuclear immunophilin FKBP39 from Drosophila melanogaster drives spontaneous liquid-liquid phase separation.
Int J Biol Macromol. 2020 Jun 30; 163:108-119.IJ

Abstract

The FKBP39 from Drosophila melanogaster is a multifunctional regulatory immunophilin. It contains two globular domains linked by a highly charged disordered region. The N-terminal domain shows homology to the nucleoplasmin core domain, and the C-terminal domain is characteristic for the family of the FKBP immunophilin ligand binding domain. The specific partially disordered structure of the protein inspired us to investigate whether FKBP39 can drive spontaneous liquid-liquid phase separation (LLPS). Preliminary analyses using CatGranule and Pi-Pi contact predictors suggested a propensity for LLPS. Microscopy observations revealed that FKBP39 can self-concentrate to form liquid condensates. We also found that FKBP39 can lead to LLPS in the presence of RNA and peptides containing Arg-rich linear motifs derived from selected nuclear and nucleolar proteins. These heterotypic interactions have a stronger propensity for driving LLPS when compared to the interactions mediated by self-associating FKBP39 molecules. To investigate whether FKBP39 can drive LLPS in the cellular environment, we analysed it in fusion with YFP in COS-7 cells. The specific distribution and diffusion kinetics of FKBP39 examined by FRAP experiments provided evidence that immunophilin is an important driver of phase separation. The ability of FKBP39 to go into heterotypic interaction may be fundamental for ribosome subunits assembly.

Authors+Show Affiliations

Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland. Electronic address: aneta.tarczewska@pwr.edu.pl.Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland.Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland.Department of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.Department of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland.Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland.Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32615218

Citation

Tarczewska, Aneta, et al. "Nuclear Immunophilin FKBP39 From Drosophila Melanogaster Drives Spontaneous Liquid-liquid Phase Separation." International Journal of Biological Macromolecules, vol. 163, 2020, pp. 108-119.
Tarczewska A, Wycisk K, Orłowski M, et al. Nuclear immunophilin FKBP39 from Drosophila melanogaster drives spontaneous liquid-liquid phase separation. Int J Biol Macromol. 2020;163:108-119.
Tarczewska, A., Wycisk, K., Orłowski, M., Waligórska, A., Dobrucki, J., Drewniak-Świtalska, M., Berlicki, Ł., & Ożyhar, A. (2020). Nuclear immunophilin FKBP39 from Drosophila melanogaster drives spontaneous liquid-liquid phase separation. International Journal of Biological Macromolecules, 163, 108-119. https://doi.org/10.1016/j.ijbiomac.2020.06.255
Tarczewska A, et al. Nuclear Immunophilin FKBP39 From Drosophila Melanogaster Drives Spontaneous Liquid-liquid Phase Separation. Int J Biol Macromol. 2020 Jun 30;163:108-119. PubMed PMID: 32615218.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nuclear immunophilin FKBP39 from Drosophila melanogaster drives spontaneous liquid-liquid phase separation. AU - Tarczewska,Aneta, AU - Wycisk,Krzysztof, AU - Orłowski,Marek, AU - Waligórska,Agnieszka, AU - Dobrucki,Jurek, AU - Drewniak-Świtalska,Magda, AU - Berlicki,Łukasz, AU - Ożyhar,Andrzej, Y1 - 2020/06/30/ PY - 2020/06/08/received PY - 2020/06/25/revised PY - 2020/06/26/accepted PY - 2020/7/3/pubmed PY - 2020/7/3/medline PY - 2020/7/3/entrez KW - FKBP39 KW - LLPS KW - Liquid-liquid phase separation SP - 108 EP - 119 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 163 N2 - The FKBP39 from Drosophila melanogaster is a multifunctional regulatory immunophilin. It contains two globular domains linked by a highly charged disordered region. The N-terminal domain shows homology to the nucleoplasmin core domain, and the C-terminal domain is characteristic for the family of the FKBP immunophilin ligand binding domain. The specific partially disordered structure of the protein inspired us to investigate whether FKBP39 can drive spontaneous liquid-liquid phase separation (LLPS). Preliminary analyses using CatGranule and Pi-Pi contact predictors suggested a propensity for LLPS. Microscopy observations revealed that FKBP39 can self-concentrate to form liquid condensates. We also found that FKBP39 can lead to LLPS in the presence of RNA and peptides containing Arg-rich linear motifs derived from selected nuclear and nucleolar proteins. These heterotypic interactions have a stronger propensity for driving LLPS when compared to the interactions mediated by self-associating FKBP39 molecules. To investigate whether FKBP39 can drive LLPS in the cellular environment, we analysed it in fusion with YFP in COS-7 cells. The specific distribution and diffusion kinetics of FKBP39 examined by FRAP experiments provided evidence that immunophilin is an important driver of phase separation. The ability of FKBP39 to go into heterotypic interaction may be fundamental for ribosome subunits assembly. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/32615218/Nuclear_immunophilin_FKBP39_from_Drosophila_melanogaster_drives_spontaneous_liquid-liquid_phase_separation L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(20)33711-9 DB - PRIME DP - Unbound Medicine ER -
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