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c-Phycocyanin primed silver nano conjugates: Studies on red blood cell stress resilience mechanism.
Colloids Surf B Biointerfaces. 2020 Jun 25; 194:111211.CS

Abstract

Green synthesis of metal-encased nutraceutical nano-hybrids has been a target for research over the last few years. In the present investigation, we have reported temperature dependent facile synthesis of silver nanoparticles using FDA approved c phycocyanin (cPC). The cPC conjugated silver nanoparticles (AgcPCNPs) were characterized by TEM, Zeta Potential, UV-vis, XPS, FTIR, and CD Spectroscopy. The temperature optimization studies suggested the synthesis of stable AgcPCNPs at 40 °C while at higher temperature system shows aggregated appearance. Molecular docking studies predicted the exclusive interaction of C, D, I, and J chains of cPC with the surface of AgNPs. Moreover, AgcPCNPs significantly (p < 0.1 %) counteract the toxic nature of AgNPs on red blood cell by measuring parameters like total RBC count, % hemolysis, % hematocrit, coagulation time, pH, electrolyte concentrations and degree of blood cell lipid peroxidation by the anti-oxidation mechanism. Skin fibroblast in vitro cell migration result suggeststhat AgcPCNPs enhanced the degree of cell movement towards the wound area. Data obtained collectively demonstrate that AgcPCNPs can be a better agent in the dermal wound healing with reduced toxicity with the bi-phasic advantage of cPC as a wound healer and Ag nano-metal as an anti-bacterial agent.

Authors+Show Affiliations

Department of Applied Physiology, School of Medicine, University of Miyazaki, Miyazaki, 889 1692, Japan. Electronic address: hkumar@med.miyazaki-u.ac.jp.Department of Applied Physiology, School of Medicine, University of Miyazaki, Miyazaki, 889 1692, Japan.Department of Chemistry, University of Miami, Coral Gables, FL, 33146, United States.Center for Collaborative Research and Community Corporation, Division of Materials Research, University of Miyazaki, Miyazaki, 889 2192, Japan.Department of Pharmaceutical Science and Technology, Birla Institute of Technology Mesra, Ranchi, 835215, India.Department of Bioengineering, Birla Institute of Technology Mesra, Ranchi, 835215, India.Department of Pharmaceutical Chemistry, NGSM Institute of Pharmaceutical Sciences, Nitte (Deemed to be) University, Paneer Campus, Mangaluru, 575018, Karnataka, India.Department of Pharmaceutical Chemistry, NGSM Institute of Pharmaceutical Sciences, Nitte (Deemed to be) University, Paneer Campus, Mangaluru, 575018, Karnataka, India.Department of Chemistry, University of Miami, Coral Gables, FL, 33146, United States.Department of Applied Physiology, School of Medicine, University of Miyazaki, Miyazaki, 889 1692, Japan.Amity Center for Nanobiotechnology and Nanomedcine (ACNN), Amity Institute of Biotechnology, Amity University, Rajasthan, Kant-Kalwar, Jaipur-Delhi Highway, Jaipur, 303002, India.Amity Center for Nanobiotechnology and Nanomedcine (ACNN), Amity Institute of Biotechnology, Amity University, Rajasthan, Kant-Kalwar, Jaipur-Delhi Highway, Jaipur, 303002, India.Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode, 638401, Tamilnadu, India.Department of Applied Physiology, School of Medicine, University of Miyazaki, Miyazaki, 889 1692, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32615521

Citation

Madhyastha, Harishkumar, et al. "C-Phycocyanin Primed Silver Nano Conjugates: Studies On Red Blood Cell Stress Resilience Mechanism." Colloids and Surfaces. B, Biointerfaces, vol. 194, 2020, p. 111211.
Madhyastha H, Madhyastha R, Thakur A, et al. C-Phycocyanin primed silver nano conjugates: Studies on red blood cell stress resilience mechanism. Colloids Surf B Biointerfaces. 2020;194:111211.
Madhyastha, H., Madhyastha, R., Thakur, A., Kentaro, S., Dev, A., Singh, S., Chandrashekharappa R, B., Kumar, H., Acevedo, O., Nakajima, Y., Daima, H. K., Aradhya, A., Nagaraj P, N., & Maruyama, M. (2020). C-Phycocyanin primed silver nano conjugates: Studies on red blood cell stress resilience mechanism. Colloids and Surfaces. B, Biointerfaces, 194, 111211. https://doi.org/10.1016/j.colsurfb.2020.111211
Madhyastha H, et al. C-Phycocyanin Primed Silver Nano Conjugates: Studies On Red Blood Cell Stress Resilience Mechanism. Colloids Surf B Biointerfaces. 2020 Jun 25;194:111211. PubMed PMID: 32615521.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - c-Phycocyanin primed silver nano conjugates: Studies on red blood cell stress resilience mechanism. AU - Madhyastha,Harishkumar, AU - Madhyastha,Radha, AU - Thakur,Abhishek, AU - Kentaro,Sakai, AU - Dev,Abhimanyu, AU - Singh,Sneha, AU - Chandrashekharappa R,Bistivalli, AU - Kumar,Hemanth, AU - Acevedo,Orlando, AU - Nakajima,Yuichi, AU - Daima,Hemant Kumar, AU - Aradhya,Akhela, AU - Nagaraj P,Navya, AU - Maruyama,Masugi, Y1 - 2020/06/25/ PY - 2020/04/07/received PY - 2020/06/04/revised PY - 2020/06/20/accepted PY - 2020/7/3/pubmed PY - 2020/7/3/medline PY - 2020/7/3/entrez KW - Anti-oxidation KW - Blood electrolytes KW - Conjugated nano-particles KW - Fibroblast cell migration KW - Haemoglobin configuration KW - Silver nanoparticles KW - Wound healing KW - c-Phycocyanin SP - 111211 EP - 111211 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 194 N2 - Green synthesis of metal-encased nutraceutical nano-hybrids has been a target for research over the last few years. In the present investigation, we have reported temperature dependent facile synthesis of silver nanoparticles using FDA approved c phycocyanin (cPC). The cPC conjugated silver nanoparticles (AgcPCNPs) were characterized by TEM, Zeta Potential, UV-vis, XPS, FTIR, and CD Spectroscopy. The temperature optimization studies suggested the synthesis of stable AgcPCNPs at 40 °C while at higher temperature system shows aggregated appearance. Molecular docking studies predicted the exclusive interaction of C, D, I, and J chains of cPC with the surface of AgNPs. Moreover, AgcPCNPs significantly (p < 0.1 %) counteract the toxic nature of AgNPs on red blood cell by measuring parameters like total RBC count, % hemolysis, % hematocrit, coagulation time, pH, electrolyte concentrations and degree of blood cell lipid peroxidation by the anti-oxidation mechanism. Skin fibroblast in vitro cell migration result suggeststhat AgcPCNPs enhanced the degree of cell movement towards the wound area. Data obtained collectively demonstrate that AgcPCNPs can be a better agent in the dermal wound healing with reduced toxicity with the bi-phasic advantage of cPC as a wound healer and Ag nano-metal as an anti-bacterial agent. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/32615521/c-Phycocyanin_primed_silver_nano_conjugates:_Studies_on_red_blood_cell_stress_resilience_mechanism L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(20)30567-1 DB - PRIME DP - Unbound Medicine ER -
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