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Impact of glucocorticoids on systemic sirtuin 1 expression and activity in rats with adjuvant-induced arthritis.
Epigenetics. 2020 Jul 13 [Online ahead of print]E

Abstract

The class III histone deacetylase sirtuin 1 (SIRT1) plays a pivotal role in numerous biological and physiological functions, including inflammation. An association between SIRT1 and proinflammatory cytokines might exist. In addition to their important role in inflammation associated with rheumatoid arthritis (RA), proinflammatory cytokines mediate the development of systemic effects. Here, we evaluated systemic SIRT1 expression and enzymatic activity, in peripheral blood mononuclear cells (PBMCs) and in liver isolated from rats with adjuvant-induced arthritis (AIA), treated or not with low or high doses of glucocorticoids (GCs). We also measured the production of tumour necrosis factor alpha (TNF) and interleukin-1 beta (IL-1 beta) in PBMCs and liver. We found that SIRT1 expression and activity increased in PBMCs of AIA rats compared to healthy controls and decreased under GC treatment. Similarly, we observed an increased SIRT1 activity in the liver of AIA rats compared to healthy controls which decreased under high doses of GCs. We also found an increase in IL-1 beta and TNF levels in the liver of AIA rats compared to healthy controls, which decreased under high doses of GC. We did not observe a significant correlation between SIRT1 activity and proinflammatory cytokine production in PBMC or liver. In contrast, a strong positive correlation was found between the liver levels of TNF and IL-1 beta (rho=0.9503, p=7.5x10-21). Our results indicate that increased inflammation in AIA rats compared to healthy control is accompanied by an increased SIRT1 activity in both PBMCs and liver, which could be decreased under GC treatment.

Authors+Show Affiliations

Pathogens & Inflammation/EPILAB Laboratory, UPRES EA 4266, SFR FED 4234, University of Franche-Comté, Université Bourgogne Franche-Comté (UBFC) , Besançon, France.Pepite EA4267, Université Bourgogne Franche-Comté (UBFC) , Besançon, France.Pathogens & Inflammation/EPILAB Laboratory, UPRES EA 4266, SFR FED 4234, University of Franche-Comté, Université Bourgogne Franche-Comté (UBFC) , Besançon, France.Pathogens & Inflammation/EPILAB Laboratory, UPRES EA 4266, SFR FED 4234, University of Franche-Comté, Université Bourgogne Franche-Comté (UBFC) , Besançon, France.Pathogens & Inflammation/EPILAB Laboratory, UPRES EA 4266, SFR FED 4234, University of Franche-Comté, Université Bourgogne Franche-Comté (UBFC) , Besançon, France.Pepite EA4267, Université Bourgogne Franche-Comté (UBFC) , Besançon, France. Department of Rheumatology, CHRU Besançon , Besançon, France.Pepite EA4267, Université Bourgogne Franche-Comté (UBFC) , Besançon, France. Department of Rheumatology, CHRU Besançon , Besançon, France.Pathogens & Inflammation/EPILAB Laboratory, UPRES EA 4266, SFR FED 4234, University of Franche-Comté, Université Bourgogne Franche-Comté (UBFC) , Besançon, France. Department of Rheumatology, CHRU Besançon , Besançon, France.Pepite EA4267, Université Bourgogne Franche-Comté (UBFC) , Besançon, France.Pathogens & Inflammation/EPILAB Laboratory, UPRES EA 4266, SFR FED 4234, University of Franche-Comté, Université Bourgogne Franche-Comté (UBFC) , Besançon, France. Department of Virology, CHRU Besançon , Besançon, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32615849

Citation

Pasquereau, Sébastien, et al. "Impact of Glucocorticoids On Systemic Sirtuin 1 Expression and Activity in Rats With Adjuvant-induced Arthritis." Epigenetics, 2020, pp. 1-12.
Pasquereau S, Totoson P, Nehme Z, et al. Impact of glucocorticoids on systemic sirtuin 1 expression and activity in rats with adjuvant-induced arthritis. Epigenetics. 2020.
Pasquereau, S., Totoson, P., Nehme, Z., Abbas, W., Kumar, A., Verhoeven, F., Prati, C., Wendling, D., Demougeot, C., & Herbein, G. (2020). Impact of glucocorticoids on systemic sirtuin 1 expression and activity in rats with adjuvant-induced arthritis. Epigenetics, 1-12. https://doi.org/10.1080/15592294.2020.1790789
Pasquereau S, et al. Impact of Glucocorticoids On Systemic Sirtuin 1 Expression and Activity in Rats With Adjuvant-induced Arthritis. Epigenetics. 2020 Jul 13;1-12. PubMed PMID: 32615849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of glucocorticoids on systemic sirtuin 1 expression and activity in rats with adjuvant-induced arthritis. AU - Pasquereau,Sébastien, AU - Totoson,Perle, AU - Nehme,Zeina, AU - Abbas,Wasim, AU - Kumar,Amit, AU - Verhoeven,Frank, AU - Prati,Clément, AU - Wendling,Daniel, AU - Demougeot,Céline, AU - Herbein,Georges, Y1 - 2020/07/13/ PY - 2020/7/4/pubmed PY - 2020/7/4/medline PY - 2020/7/4/entrez KW - AIA KW - IL-1 beta KW - PBMCs KW - SIRT1 KW - Sirtuin 1 KW - TNF alpha KW - adjuvant-induced arthritis KW - liver SP - 1 EP - 12 JF - Epigenetics JO - Epigenetics N2 - The class III histone deacetylase sirtuin 1 (SIRT1) plays a pivotal role in numerous biological and physiological functions, including inflammation. An association between SIRT1 and proinflammatory cytokines might exist. In addition to their important role in inflammation associated with rheumatoid arthritis (RA), proinflammatory cytokines mediate the development of systemic effects. Here, we evaluated systemic SIRT1 expression and enzymatic activity, in peripheral blood mononuclear cells (PBMCs) and in liver isolated from rats with adjuvant-induced arthritis (AIA), treated or not with low or high doses of glucocorticoids (GCs). We also measured the production of tumour necrosis factor alpha (TNF) and interleukin-1 beta (IL-1 beta) in PBMCs and liver. We found that SIRT1 expression and activity increased in PBMCs of AIA rats compared to healthy controls and decreased under GC treatment. Similarly, we observed an increased SIRT1 activity in the liver of AIA rats compared to healthy controls which decreased under high doses of GCs. We also found an increase in IL-1 beta and TNF levels in the liver of AIA rats compared to healthy controls, which decreased under high doses of GC. We did not observe a significant correlation between SIRT1 activity and proinflammatory cytokine production in PBMC or liver. In contrast, a strong positive correlation was found between the liver levels of TNF and IL-1 beta (rho=0.9503, p=7.5x10-21). Our results indicate that increased inflammation in AIA rats compared to healthy control is accompanied by an increased SIRT1 activity in both PBMCs and liver, which could be decreased under GC treatment. SN - 1559-2308 UR - https://www.unboundmedicine.com/medline/citation/32615849/Impact_of_glucocorticoids_on_systemic_sirtuin_1_expression_and_activity_in_rats_with_adjuvant-induced_arthritis L2 - http://www.tandfonline.com/doi/full/10.1080/15592294.2020.1790789 DB - PRIME DP - Unbound Medicine ER -
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